CAO Jun , CHEN Ping , YANG Yue , PENG Hong , CHEN Yan , LIU Shaokun.
  • The Division of Respiratory Disease, The Department of Internal Medicine, The Second Xiangya Hospital, CentralSouth University;
小鼠; 骨髓; 單核細胞; 內皮祖細胞; 密度梯度離心; 全骨髓培養
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【摘要】 目的 比較密度梯度離心法及全骨髓培養法分離培養內皮祖細胞的差異。方法 取4周雄性近交系C57BL/6J小鼠骨髓,分別使用密度梯度離心法及全骨髓培養法培養,觀察細胞貼壁情況和細胞形態,并行DiIacLDL及FITCUEAI雙染、vWF、eNOS及細胞表面標志檢測。結果 密度梯度離心法培養細胞可形成典型鋪路石樣改變及形成血管樣結構;而全骨髓培養法貼壁細胞形態多樣,較多呈長梭形鋪展生長,部分細胞呈類圓形及紡錘形。比較兩種方法培養細胞攝取DiIacLDL、結合FITCUEAI雙陽性率以及vWF、eNOS及細胞表面標志表達陽性率,差別均有統計學意義(P lt;005)。應用密度梯度離心法,隨著培養時間延長,表達CD34、CD133及FLk1細胞逐漸增多(P lt;005)。結論 密度梯度離心法及全骨髓培養法在EGM2MV培養體系下均可培養出內皮祖細胞,但密度梯度離心法較全骨髓培養法培養的內皮祖細胞純度高。

Citation： CAO Jun,CHEN Ping,YANG Yue,PENG Hong,CHEN Yan,LIU Shaokun.. Comparison between Two Culture Mothods of Endothelial Progenitor Cells Derived from Mice Bone Marrow. West China Medical Journal, 2010, 25(3): 520-. doi: Copy

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