Clinical Significance and Expression of the Human Telomerase Gene in Cytological Specimens of Cervix_West China Medical Journal

Authors：

MA Qingsong ¹ , FENG Lubing ¹ , WANG Min ¹ , CHEN Bing ¹ , REN Yubo ²

1. Affiliated Second People’s Hospital of Taishan Medical College, Liaocheng, Shandong 252600, P.R.China; 2.Department of Pathology, People’s Hospital of Liaocheng, Liaocheng, Shandong 252000, P.R.China;

Corresponding?author：

Keywords：

人類染色體端粒酶基因; 熒光原位雜交; 宮頸癌; 宮頸上皮內瘤變; 宮頸炎

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

【摘要】目的探討宮頸上皮內瘤變（CIN）和宮頸鱗癌（SCC）組織中人類染色體端粒酶基因（hTERC）的表達和臨床意義。方法收集2007年10月-2009年6月經病理學證實的116例宮頸脫落細胞標本，其中LSIL（CINⅠ）30例、HSIL（CINⅡ/Ⅲ）37例、SCC 16例、宮頸炎33例，用熒光原位雜交（FISH）方法檢測脫落細胞hTERC基因。結果在宮頸炎、LSIL、HSIL和SCC組中hTERC基因的表達率分別是6.1%、16.7%、51.4%和93.8%，其中，HSIL、SCC組與宮頸炎組比較，hTERC基因陽性率差異有統計學意義（P lt;0.05），LSIL組與HSIL組比較、LSIL組與SCC組比較、HSIL組與SCC組比較，差異有統計學意義（P lt;0.05），且隨著病變程度增加，hTERC基因表達率增加。結論 hTERC基因在細胞學LSIL、HSIL和SCC中表達異常，且隨病變程度增加陽性表達率也增加，可作為宮頸癌癌前病變進展的生物遺傳學監測指標，并有望成為宮頸癌早期篩查方法之一。
【Abstract】 Objective To explore the clinical significance and expression of the human telomerase gene (hTERC) in the cervical intraepithelial neoplasia (CIN) and squamous carcinoma of the cervix (SCC). Methods According to histological biopsy from October 2007 to June 2009, 116 pap smears were divided into LSIL (n=30), HSIL (n=37), SCC (n=16), and cervicitis (n=33) groups. Fluorescence in situ hybridization (FISH) was used to detect the expression of hTERC. Results Positive expression rate of hTERC was 6.1% in cervicitis group, 16.7% in LSIL group, 51.4% in HSIL group, and 93.8% in SCC group, respectively. Compared to cervicitis group, the expression of hTERC in HSIL and SCC groups was significantly higher (P lt;0.05). Among LSIL, HSIL, and SCC groups, there were significant differencec in hTERC expression between every two groups (P lt;0.05). From LSIL to SCC, the expression of hTERC increased obviously. Conclusion Abnormal expression of hTERC exists in LSIL, HSIL, and SCC patients, which significantly increases during malignant development. It may be a biogenetics monitor index of cervical precancerosis and will be a screening marker for cervical cancer.

Citation： MA Qingsong,FENG Lubing,WANG Min,CHEN Bing,REN Yubo. Clinical Significance and Expression of the Human Telomerase Gene in Cytological Specimens of Cervix. West China Medical Journal, 2010, 25(7): 1313-1316. doi: Copy

1.	Heselmeyer-Haddad K, Sommerfeld K, White NM, et al. Genomic amplification of the human telomerase gene (TERC) in pap smears predicts the development of cervical cancer[J]. Am J Pathol, 2005, 166(4): 1229-1238.
2.	劉開江, 美熱古麗·阿不拉, 劉繼文, 等. 新疆維、漢婦女宮頸癌人類染色體端粒酶基因的研究[J]. 新疆醫科大學學報, 2008, 31(10): 1342-1344.
3.	Kim NW, Piatyszek MA, Prowse KR, et al. Specific association of human telomerase activity with immortal cells and cancer[J]. Science, 1994, 266(5193): 2011-2015.
4.	楊俊玲, 李長江, 肖永紅. 端粒酶在宮頸癌組織中的表達及化療對其影響[J]. 現代預防醫學, 2008, 35(17): 3452-3455.
5.	初彥輝, 武艷, 袁曉環, 等. 端粒、端粒酶在腫瘤、細胞衰老中的研究[J]. 中國醫療前沿, 2009, 4(1): 4-8.
6.	Feng J, Funk WD, Wang SS, et al. The RNA component of human telomerase[J]. Science, 1995, 269(5228): 1236-1241.
7.	Jarboe EA, Liaw KL, Thompson LC, et al. Analysis of telomerase as a diagnostic biomarker of cervical dysplasia and carcinoma[J]. Oncogene, 2002, 21(4): 664-673.
8.	Nagai N, Ohita T, Murakami J, et al. Semiquantitative analysis of telomerase activity in cervical cancer and precancerous lesion[J]. Oncol Rep, 1999, 6(2): 325-328.
9.	Heselmeyer-Haddad K, Janz V, Castle PE, et al. Detection of genomic amplification of the human telomerase gene(TERC)in cytologic specimens as a genetic test for the diagnosis of cervical dysplasia[J]. Am J Pathol, 2003, 163(4): 1405-1416.
10.	趙富璽, 孫瑞芳, 王晉芬, 等. HPV16感染與端粒酶hTERC、P21waf、Ki67表達在宮頸嶙狀細胞癌及其癌前病變組織中的關系及意義[J]. 大同醫學專科學校學報, 2005, 4(4): 1-4.
11.	孫信, 薛敏, 鄧新糧. 宮頸病變中端粒酶活性與高危HPV感染關系的研究[J]. 實用預防醫學, 2008, 15(4): 1021-1024.

1. Heselmeyer-Haddad K, Sommerfeld K, White NM, et al. Genomic amplification of the human telomerase gene (TERC) in pap smears predicts the development of cervical cancer[J]. Am J Pathol, 2005, 166(4): 1229-1238.
2. 劉開江, 美熱古麗·阿不拉, 劉繼文, 等. 新疆維、漢婦女宮頸癌人類染色體端粒酶基因的研究[J]. 新疆醫科大學學報, 2008, 31(10): 1342-1344.
3. Kim NW, Piatyszek MA, Prowse KR, et al. Specific association of human telomerase activity with immortal cells and cancer[J]. Science, 1994, 266(5193): 2011-2015.
4. 楊俊玲, 李長江, 肖永紅. 端粒酶在宮頸癌組織中的表達及化療對其影響[J]. 現代預防醫學, 2008, 35(17): 3452-3455.
5. 初彥輝, 武艷, 袁曉環, 等. 端粒、端粒酶在腫瘤、細胞衰老中的研究[J]. 中國醫療前沿, 2009, 4(1): 4-8.
6. Feng J, Funk WD, Wang SS, et al. The RNA component of human telomerase[J]. Science, 1995, 269(5228): 1236-1241.
7. Jarboe EA, Liaw KL, Thompson LC, et al. Analysis of telomerase as a diagnostic biomarker of cervical dysplasia and carcinoma[J]. Oncogene, 2002, 21(4): 664-673.
8. Nagai N, Ohita T, Murakami J, et al. Semiquantitative analysis of telomerase activity in cervical cancer and precancerous lesion[J]. Oncol Rep, 1999, 6(2): 325-328.
9. Heselmeyer-Haddad K, Janz V, Castle PE, et al. Detection of genomic amplification of the human telomerase gene(TERC)in cytologic specimens as a genetic test for the diagnosis of cervical dysplasia[J]. Am J Pathol, 2003, 163(4): 1405-1416.
10. 趙富璽, 孫瑞芳, 王晉芬, 等. HPV16感染與端粒酶hTERC、P21waf、Ki67表達在宮頸嶙狀細胞癌及其癌前病變組織中的關系及意義[J]. 大同醫學專科學校學報, 2005, 4(4): 1-4.
11. 孫信, 薛敏, 鄧新糧. 宮頸病變中端粒酶活性與高危HPV感染關系的研究[J]. 實用預防醫學, 2008, 15(4): 1021-1024.

West China Medical Journal

Clinical Significance and Expression of the Human Telomerase Gene in Cytological Specimens of Cervix

Abstract Full text Figures/Tables Video References Cited by

Format

Content