【摘要】 目的  探討機采血小板在實驗室內保存期間的生化改變及其意義。 方法  采用ROCHE cobas b 221和OLYMPUS AU400測定血小板樣本的pH值、PO2、PCO2、葡萄糖（GLU）濃度、乳酸脫氫酶（LDH）釋放量。 結果  保存期間pH值由7.311（第1天）降至7.116（第5天）；PO2波動于106.7～119.5 mm Hg（1 mm Hg=0.133 kPa）；PCO2波動于25.0～35.8 mm Hg；GLU濃度呈進行性下降，由18.8 mmol/L降至15.2 mmol/L；LDH濃度顯著增加，由150 mmol/L（第1天）增至259 mmol/L（第5天）。 結論  血小板在保存期內，其生化參數會隨著保存時間發生改變。而這些參數作為間接指標對機采血小板是否被細菌污染具有一定的指導意義。
【Abstract】 Objective  To observe the biochemistry changes of apheresis platelets (AP), including pH, PO2, PCO2, GLU, LDH, within the storage time and its significance. Methods  The pH, PO2, PCO2, GLU and LDH of platelet samples were detected by ROCHE cobas b 221 and OLYMPUS AU400. Results  During the storage time, the pH value of platelet decreased gradually from 7.311 (the first day) to 7.116 (the fifth day); the PO2 fluctuated from 106.7 mm Hg (1 mm Hg=0.133 kPa) to 119.5 mm Hg; the PCO2 fluctuated from 25.0 mm Hg to 35.8 mm Hg;the concentration of GLU decreased from 18.8 mmol/L to 15.2 mmol/L;the concentration of LDH increased observably from 150 mmol/L (the first day) to 259 mmol/L (the fifth day). Conclusion  There were observably changes of biochemistry of the AP during storage time, and these changes may be the indicators of platelets contamination.

Citation： WANG Lixin,HUANG Chunyan,QIN Li,CAI Juan,XU Kehe,TAN Bin,CHEN Long. The Biochemistry Changes of Apheresis Platelets within the Storage Time. West China Medical Journal, 2010, 25(9): 1689-1690. doi: Copy

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