Establishment of the Model of Local Mesenteric Venous Thrombosis in Rabbits_West China Medical Journal

Authors：

YANG Jun , SUO Zhijun , HE Jiangyun , KE Qi , ZHANG Zhiwei

Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R.China;

Corresponding?author：

Keywords：

腸系膜; 局部性靜脈血栓; D-二聚體; 動物模型

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【摘要】目的 探討建立局部性家兔腸系膜靜脈血栓（MVT）模型的方法。方法于2008年1月，將36只家兔隨機均分為3組，腸系膜前靜脈局部阻斷加靜脈分支注射凝血酶，A組80 U/mL，B組40 U/mL，C組注射生理鹽水1 mL。觀測血栓形成時間、范圍和周圍靜脈血D-二聚體（DD）變化。結果 A、B兩組均可建立控制范圍內穩定MVT模型，血栓形成時間分別為（15.6±2.0） min和（22.3±2.5）min，兩組比較有統計學意義（P lt;0.001）；術前A、B兩組DD為（68.4±5.7）ng/mL、（72.7±6.4）ng/mL，血栓形成后為（209.0±24.0）ng/mL、（215.4±17.6）ng/mL，組內比較有統計學意義（P lt;0.001）；C組不形成血栓。結論局部靜脈阻斷加凝血酶注射法可建立穩定和范圍可控的MVT模型，建模前后血漿DD值有一定實驗參考價值。
【Abstract】 Objective To establish the model of local mesenteric venous thrombosis (MVT) in rabbits. Methods In January 2008, 36 rabbits were randomly divided into three groups with 12 rabbits in each. The anterior mesenteric veins were blocked regionally, and at the same time the rabbits were injected with thrombin 80 U/mL through the branches of mesenteric vein in group A, 40 U/mL in group B, and 1 mL normal saline in group C. Time and range of thrombosis were observed, and D-dimer level in peripheral vein was tested. Results Stable MVT model was established in intended region in both group A and B. There was a significant reduction of thrombosis time in group A [(15.6±2.0) minutes] when compared with that in group B [(22.3±2.5) minutes] (P lt;0.001). The levels of D-dimer in group A two hours after operation [(209.0±24.0) ng/mL] increased significantly compared with that before the operation [(68.4±5.7) ng/mL](P lt;0.001); while the same condition was found in group B [(215.4±17.6) ng/mL vs (72.7±6.4) ng/mL] (P lt;0.001). No thrombus was found in group C. Conclusion Stable MVT model with controllable thrombotic range can be established by regional vein blockade plus thrombin injection. Plasma D-dimer levels before and after model establishment could be as a parameter for assessing the experiment.

Citation： YANG Jun,SUO Zhijun,HE Jiangyun,KE Qi,ZHANG Zhiwei. Establishment of the Model of Local Mesenteric Venous Thrombosis in Rabbits. West China Medical Journal, 2010, 25(10): 1827-1830. doi: Copy

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13.	Righini M, Perrier A, De Moerloose P, et al. D-dimer for venous thromboembolism diagnosis: 20 years later[J]. J Thromb Haemost, 2008, 6(7): 1059-1071.
14.	Palareti G, Cosmi B, Legnani C, et al. D-dimer testing to determine the duration of anticoagulation therpy[J]. N Engl J Med, 2006, 355(17): 1780-1789.
15.	Gorog DA. Prognostic value of plasma fibrinolysis activation markers in cardiovascular disease[J]. J Am Coll Cardiol, 2010, 55(24): 2701-2709.

1. Rhee RY, Gloviczki P, Mendonca CT, et al. Mesenteric venous thrombosis: still a lethal disease in the 1990[J]. J Vasc Surg, 1994, 20(5): 688-697.
2. James AW, Rabl C, Westphalen AC, et al. Portomesenteric venous thrombosis after laparoscopic surgery: a systematic literature review[J]. Arch Surg, 2009, 144(6): 520-526.
3. Brunaud L, Antunes L, Adler SC, et al. Acute mesenteric venous thrombosis: Case for nonoperative management[J]. J Vas Surg, 2001, 34(4): 673-679.
4. Wang MQ, Guo LP, Lin HY, et al. Transradial approach for transcatheter selective superior mesenteric artery urokinase infusion therapy in patients with acute extensive portal and superior mesenteric vein thrombosis[J]. Cardiovasc Intervent Radiol. 2010, 33(1): 80-89.
5. Acosta S, Ogren M, Sternby NH, et al. Mesenteric venous thrombosis with transmural intestinal infarction: a population-based study[J]. J Vasc Surg, 2005, 41(1): 59-63.
6. Acosta S, Alhadad A, Svensson P, et al. Epidemiology, risk and prognostic factors in mesenteric venous thrombosis[J]. Br J Surg, 2008, 95(10): 1245-1251.
7. Acosta S. Epidemiology of mesenteric vascular disease: clinical implications[J]. Semin Vasc Surg, 2010, 23(1): 4-8.
8. Dentali F, Ageno W, Witt D, et al. Natural history of mesenteric venous thrombosis in patients treated with vitamin K antagonists: a multi-centre, retrospective cohort study[J]. Thromb Haemost, 2009, 102(3): 501-504.
9. Levi M, Dörffle-Melly J, Johnson GJ, et al. Usefulness and limitations of animal models of venous thrombosis[J]. Thromb Haemost, 2001, 86(5): 1331-1333.
10. Schumacher WA, Bostwick JS, Stewart AB, et al. Effect of the direct factor Xa inhibitor apixaban in rat models of thrombosis and hemostasis[J]. J Cardiovasc Pharmacol, 2010, 55(6): 609-616.
11. Hayashi T, Mogami H, Murakami Y, et al. Real-time analysis of platelet aggregation and procoagulant activity during thrombus formation in vivo[J]. Pflugers Arch, 2008, 456(6): 1239-1251.
12. Aguejouf O, Doutremepuich F, Doutremepuich C. Effects of external electrical stimulation on laser-beam-induced experimental thrombosis[J]. Pathophysiol Haemost Thromb, 2006, 35(5): 364-369.
13. Righini M, Perrier A, De Moerloose P, et al. D-dimer for venous thromboembolism diagnosis: 20 years later[J]. J Thromb Haemost, 2008, 6(7): 1059-1071.
14. Palareti G, Cosmi B, Legnani C, et al. D-dimer testing to determine the duration of anticoagulation therpy[J]. N Engl J Med, 2006, 355(17): 1780-1789.
15. Gorog DA. Prognostic value of plasma fibrinolysis activation markers in cardiovascular disease[J]. J Am Coll Cardiol, 2010, 55(24): 2701-2709.

West China Medical Journal

Establishment of the Model of Local Mesenteric Venous Thrombosis in Rabbits

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