Comparison between Assay of Immunoblotting Test, ELISA and RIA in Detection for Islet Autoantibodies_West China Medical Journal

Authors：

ZHANG Yu , CHEN Ling , XIONG Zhongyun , et al

Division of Hormone in Department of Laboratory, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding?author：

Keywords：

免疫印跡法; 糖尿病; 谷氨酸脫羧酶抗體; 胰島細胞抗體

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目的:評價免疫印跡法檢測胰島自身抗體（GAD-A、ICA、IAA）與酶聯免疫法測ICA、GAD-A放射免疫法測IAA結果的一致性。方法:采用免疫印跡法測定81例糖尿病患者胰島自身抗體,將結果與酶聯免疫法測定的GAD-A、ICA,放射免疫法測定IAA結果進行比較。結果:免疫印跡法陽性檢出率為:GAD-A 51.8%,ICA 18.5%,IAA 27.1%；酶聯免疫法（GAD-A、ICA）、放射免疫法（IAA）陽性檢出率:GAD-A 32.1%,ICA 34.5%,IAA 30.8%；上述兩組結果進行比較,兩組相比ICA和GAD-A有統計學差異（P lt;0.05）,IAA無統計學差異。兩組結果一致率比較:GAD-A 50.6%,ICA 64.2%,IAA 69.1%。結論:與臨床常用酶聯免疫法檢測GAD-A、ICA,放射免疫法檢測IAA比較,免疫印跡法和酶聯免疫法在ICA及GAD-A陽性檢出率上的差異有顯著性,和放射免疫法在IAA陽性檢出率上差異無顯著性。

Citation： ZHANG Yu,CHEN Ling,XIONG Zhongyun,et al. Comparison between Assay of Immunoblotting Test, ELISA and RIA in Detection for Islet Autoantibodies. West China Medical Journal, 2009, 24(3): 697-699. doi: Copy

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3.	鄧智勇,劉英,安振梅.三聯蛋白芯片法在胰島自身抗體檢測中的臨床應用[J].華西醫學,2006,21（1）:92-93..
4.	張慶俠,郝建華,胡鋒蘭,等.免疫印跡法檢測糖尿病患者自身抗體的臨床應用[J].實用醫學雜志,2007,23（13）:2093-2094..
5.	張慶俠,郝建華,程瓊,等.3種方法檢測胰島素自身抗體的結果比較[J].實用全科醫學,2008,6（5）:526-527..
6.	TOM C, LANDINOLSSON M, LERNAMARK A, et al. Prognostic factors for the course of β cell function in autoimmune diabetes[J]. Clin Endocrinol Metab,2000,85（12）:4619-4623..
7.	TUOMI T, GROOP L C, ZIMMET P Z, et al. Antibodies to glutamic acid decarboxylase reveal latent autoimmune diabetes mellitus in adults with a noninsulindependent onset of disease [J].Diabetes,1993,42（2）:359-362..

1. VERGE C F, STERNGER D, BONIFACIO E, et al. Combined use of autoantibodies（IA2 autoantibody, GAD antibody, insulin autoantibody, cytoplasmic islet cell antibodies）in type 1 diabetes: Combinational Islet Autoantibody Workshop [J]. Diabetes,1998,47（5）:1857-1866..
2. YAMAMOTO A M, DESCHAMPS I, GARCHON H J, et al. Young age and HLA markers enhance the risk of progression to type 1 diabetes in antibodypositive siblings of diabetic children [J]. J Autoimmun,1998,11（6）:643-650..
3. 鄧智勇,劉英,安振梅.三聯蛋白芯片法在胰島自身抗體檢測中的臨床應用[J].華西醫學,2006,21（1）:92-93..
4. 張慶俠,郝建華,胡鋒蘭,等.免疫印跡法檢測糖尿病患者自身抗體的臨床應用[J].實用醫學雜志,2007,23（13）:2093-2094..
5. 張慶俠,郝建華,程瓊,等.3種方法檢測胰島素自身抗體的結果比較[J].實用全科醫學,2008,6（5）:526-527..
6. TOM C, LANDINOLSSON M, LERNAMARK A, et al. Prognostic factors for the course of β cell function in autoimmune diabetes[J]. Clin Endocrinol Metab,2000,85（12）:4619-4623..
7. TUOMI T, GROOP L C, ZIMMET P Z, et al. Antibodies to glutamic acid decarboxylase reveal latent autoimmune diabetes mellitus in adults with a noninsulindependent onset of disease [J].Diabetes,1993,42（2）:359-362..

West China Medical Journal

Comparison between Assay of Immunoblotting Test, ELISA and RIA in Detection for Islet Autoantibodies

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