Research of Real-Time Fluorescence PCR Detection on Dengue Virus_West China Medical Journal

Authors：

YAO Jinxiu ^1,2 , ZHAO Wei ¹ , ZHANG Ling ¹ , LI Yujing ¹ , LIN Peizheng ³

1. Virology Institute, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515; 2. Clinical Laboratory, Yangjiang People’s Hospital 529500; 3. Guangzhou Traditional Chinese Medicine University, Guangzhou 510006, China;

Corresponding?author：

Keywords：

登革病毒; Taqman 探針; 實時PCR

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目的:建立登革病毒的熒光定量PCR檢測技術。方法:針對1～4型登革病毒3′端非編碼區的一段高度保守序列設計引物和探針,建立登革病毒實時熒光PCR 檢測方法及定量的檢測方法。并對10例ELISA法檢測為登革病毒陽性的病例,取其發熱1～2天的臨床血清標本進行熒光定量PCR 檢測。結果:實時熒光PCR 檢測1～4 型登革病毒均為陽性,該探針和引物是登革病毒檢測的通用探針和引物。實時熒光PCR檢測10份臨床血清,6份為陽性,陽性率為60%。結論:實時PCR方法是一個快速、特異性強、敏感性高的檢測登革病毒的方法,適用于登革熱的臨床早期診斷,具有很好的社會效益和經濟效益。

Citation： YAO Jinxiu ,ZHAO Wei,ZHANG Ling,LI Yujing,LIN Peizheng. Research of Real-Time Fluorescence PCR Detection on Dengue Virus. West China Medical Journal, 2009, 24(4): 912-914. doi: Copy

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1. ROBERT V G, DAVID W V. Dengue: an escalating problem[J]. BMJ,2002,324:1563-1566..
2. GUZMAN M G, KOURI G. Dengue and dengue hemorrhagic fever in the Americas: lessons and challenges[J]. J Clin Virol,2003,27(1):1213..
3. SCHWARTZ E, MILEGUIR F, GROSSMAN Z, et al. Evaluation of ELISA-based Sero-diagnosis of Dengue fever in travelers[J]. J Clin Virol,2000,19 (3):169-173..
4. DROSTEN C, GOTTIG S, SCHILLING S, et al. Rapid detection and quantification of RNA of ebola and marburg viruses, lassa virus, crimean-congo hemorrhagic fever virus, rift alley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR[J]. J Clin Microbiol, 2002, 40(7): 2323-2330..
5. CALLAHAN J D, WU-SJ-L. Development and evaluation of sero-type and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus[J]. J Clin Microbiol,2001,39 (11):4119-4124..
6. YONG Y K, CHONG H T, TAN C T, et al. Rapid detection sero-typing and quantitation of dengue viruses by TaqMan real-time one-step RT-PCR[J]. J Virol Met hods,2006,138 (1):123-130..

West China Medical Journal

Research of Real-Time Fluorescence PCR Detection on Dengue Virus

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