Effect of Ecdysterone on Protein Expression of IRS1/2 in Insulinresistant HepG2 Cells_West China Medical Journal

Authors：

1.the Department of Endocrinology, Affiliated Hospital of Luzhou Medical College, Luzhou Sichuan 646000, China; 2. the people’s Hospital of Ziyang city, Ziyang Sichuan 641300;3. the Department of Pharmacology, Chonyqing Medical University, Chongqing 400016, China;

Corresponding?author：

Keywords：

蛻皮甾酮; 胰島素抵抗; 細胞模型; 胰島素受體底物

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目的:探討蛻皮甾酮對胰島素抵抗(IR)HepG2細胞胰島素受體底物(IRS)蛋白表達的影響。方法:胰島素抵抗HepG2細胞模型建立后,培養液中加入蛻皮甾酮共同孵育,觀察蛻皮甾酮及吡格列酮對模型細胞葡萄糖摻入率的影響;應用免疫細胞化學染色法等方法觀察蛻皮甾酮對胰島素抵抗HepG2細胞IRS-1、IRS-2表達的影響。結果:與模型細胞組比較,1×10-5mol/L蛻皮甾酮可使IR HepG2細胞IRS-1、IRS-2蛋白的表達顯著增加。結論:蛻皮甾酮的胰島素增敏作用可能與胰島素信號轉導分子IRS-1、IRS-2蛋白的表達增強有關。

Citation： CHEN Qiu,ZHU Yuxia,Liu Xuepeng,et al.. Effect of Ecdysterone on Protein Expression of IRS1/2 in Insulinresistant HepG2 Cells. West China Medical Journal, 2009, 24(7): 1772-1775. doi: Copy

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11.	DONG X, PARK S, LIN X, et al. Irs1 and Irs2 signaling is essential for hepatic glucose homeostasis and systemic growth[J]. J Clin Invest,2006,116(1):101-114..
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1. DINAN L, LAFONT R. Effects and applications of arthropod steroid hormones (ecdysteroids) in mammals[J]. J Endocrinol,2006,191(1):1-8..
2. 陳秋,夏永鵬,邱宗蔭.蛻皮甾酮對胰島素抵抗細胞模型胰島素敏感性和糖代謝的影響[J].中國藥理學通報,2006,22(4):460-464..
3. 陳秋,鄒德平,張蓉,等.蛻皮甾酮對胰島素抵抗HepG2細胞胰島素受體表達的影響[J].山東醫藥,2008,48(4):13-15..
4. 陳秋,夏永鵬,邱宗蔭.吡格列酮對HepG2胰島素抵抗細胞模型的藥理學評價[J].中國藥理學通報,2006,22(2):248-251..
5. THIRONE A C, HUANG C, KLIP A. Tissuespecific roles of IRS proteins in insulin signaling and glucose transport Ⅰ[J]. Trends Endocrinol Metab,2006,17(2):77-78..
6. EGAWA K, NAKASHIMA N, SHARMA P M, et al. Persistent activation of phosphatidylinositol 3kinase causes insulin resistance due to accelerated insulin induced insulin receptor substrate1 degradation in 3T3L1 adipocytes[J]. Endocrinology,2000,141:1930-1935..
7. ZHANDE R, MITCHELL J J, WU J, et al. Molecular mechanism of insulininduced degradation of insulin receptor substrate 1[J]. Mol Cell Biol,2002,2:1016-1026..
8. PEDERSON T M, KRAMER D L, RONDINONE C M. Serine/threonine phosphorylation of IRS1 triggers its degradation:possible regulation by tyrosine phosphorylation[J]. Diabetes,2001,50:24-31..
9. AGUIRRE V, WERNER E D, GIRAUD J, et al. Phosphorylation of Ser307 in insulin receptor substrate1 blocks interactions with the insulin receptor and inhibits insulin action[J]. J Biol Chem,2002,277:1531-1537.
10. YUAN L, ZIEGLER R, HAMANM A. Inhibition of the phosphoenolpyruvate carboxykinase gene expression by metformin in cultured hepatocytes[J]. Chin Med J,2002,115:1843-1848..
11. DONG X, PARK S, LIN X, et al. Irs1 and Irs2 signaling is essential for hepatic glucose homeostasis and systemic growth[J]. J Clin Invest,2006,116(1):101-114..
12. CHEN Q, XIA Y P, QIU Z Y. Effect of ecdysterone on glucose metabolism in vitro[J]. Life Sciences,2006,78(10):1108-1113..

West China Medical Journal

Effect of Ecdysterone on Protein Expression of IRS1/2 in Insulinresistant HepG2 Cells

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