• 1State Key Laboratory of Biography,West China Hospital,West China Medical School, Sichuan University,Chengdu 610041,China;2Department of Nephrology,West China Hospital,West China Medical School, Sichuan University,Chengdu 610041,China;3Southwest National University, Chengdu Sichuan 610041,China;
血管平滑肌細胞; 血管緊張素Ⅱ; 激活轉錄因子; NOX1VSMCs; AngⅡ; ATF1; NOX1
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摘要:目的: 探討激活轉錄因子(ATF1)在血管緊張素Ⅱ(AngⅡ)誘導血管平滑肌細胞(VSMCs)中NOX1基因表達增加的作用。 方法 :體外培養大鼠主動脈VSMCs,用熒光實時定量逆轉錄PCR(Realtime RTPCR)檢測NOX1基因表達的量,Western Blot檢測ATF1蛋白在AngⅡ的刺激是否引起NOX1基因的高表達并用RNA干擾(RNAi)技術轉染VSMCs使ATF1基因沉默來觀察NOX1的表達。 結果 :AngⅡ能夠誘導 NOX1基因的表達增加以及增強ATF1的磷酸化及活性,ATF1基因沉默反過來可抑制AngⅡ誘導的NOX1基因表達的增加。 結論 :在大鼠的VSMCs中,ATF1是介導NOX1基因表達的一個必須的轉錄因子。
Abstract: Objective: To detect the role of activating transcription factor (ATF1) involved in angiotensinⅡ(AngⅡ) stimulated NOX1 gene expression.Methods :Rat aortic vascellum smooth muscle cells(VSMCs) were cultured in vitro.Use Realtime RTPCR to measure the expression of NOX1 gene.Western Blot Analysis was carried out to test the activity of ATF1 protein. RNA interference was used and transfected into VSMCs to knockdown ATF1 gene expression, and then measured NOX1 gene expression.Results : AngⅡ stimulated NOX1 gene expression and phosphorylation of ATF1 Gene silencing of ATF1 attenuated the upregulation of NOX1 mRNA by AngⅡ. Conclusion :ATF1 is an essential transcription factor that mediates expression of NOX1 gene in VSMCs by AngⅡ.

Citation： SHI Gang,MI Xuhua,LUO Can,et al.. Induction of NOX1 Gene Expression by AngⅡ with a ATF1 Dependent Manner. West China Medical Journal, 2009, 24(10): 2607-. doi: Copy

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