Effects of Immature Dendritic Cell Transfected by TGF-β1 Recombinated Adenovirus Vector on T Lymphocyte in Peripheral Blood and Allograft af ter Small Bowel Transplantation in Rat_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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LI Jipeng , HUANG Yi , WANG Weizhong , DONG Guanglong , DU Jianjun , CHEN Dongli , JI Gang , LIU Ji

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Keywords：

轉化生長因子β1,小腸移植,T 細胞

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目的　探討經轉化生長因子-β1 ( TGF-β1) 基因修飾的未成熟樹突狀細胞(imDC) 預處理大鼠小腸移
植受體后的外周血及移植腸浸潤T 細胞的變化及意義。方法　選用近交系F344/ N 和BN 大鼠建立全小腸異位移
植模型,實驗分4 組(每組24 只) : 同基因移植組(BN-BN 組) 、異基因移植組( F344/ N-BN 組) 、異基因移植+ TGF-
β1 基因轉染imDC 組( F344/ N-BN + TGF2β1 組) 和異基因移植+ TGF-β1 基因轉染imDC + FK506 組( F344/ N-
BN + TGF-β1 + FK506 組) 。各組大鼠分別于術后3 、5 、7 d 各處死6 只,獲取大鼠靜脈血和移植腸。應用免疫組化
SABC 法檢測受體鼠外周血及移植腸CD4 + 、CD8 + 、CD25 + 細胞和IL-4 的表達。同時行移植腸組織病理學檢查
并觀察大鼠生存情況。結果　TGF-β1 修飾的DC 細胞能顯著抑制外周血及移植腸浸潤淋巴細胞CD4 + 、CD8 + 及
CD25 + 的表達,并提高IL-4 的表達; 顯著延長受體大鼠的生存時間,但移植腸仍有排斥反應的病理組織學征象。
結論　TGF-β1 修飾的DC 通過影響受體外周血及移植腸浸潤T 細胞對大鼠小腸移植發揮免疫抑制作用。

Citation： LI Jipeng,HUANG Yi,WANG Weizhong,DONG Guanglong,DU Jianjun,CHEN Dongli,JI Gang,LIU Ji,.,Department of Gastrointestinal Surgery,Xijing Hospital,The Fourth Military Medical University,Xian,China Corresponding Author WANG Weizhong,Email weichangfmmu.edu.cn. Effects of Immature Dendritic Cell Transfected by TGF-β1 Recombinated Adenovirus Vector on T Lymphocyte in Peripheral Blood and Allograft af ter Small Bowel Transplantation in Rat. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(2): 96-101. doi: Copy

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3.	Fairchild PJ,Waldmann H.Dendritic cells and prospects for transplantation tolerance[J].Curr Opin Immunol,2000; 12(5):528.
4.	Hackstein H,Morelli AE,Thomson AW.Designer dendritic cells for tolerance induction:guided not misguided missiles[J].Trends Immunol,2001; 22(8):437.
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7.	董光龍,李開宗,王為忠,等.雙Cuff雙造口大鼠異位小腸移植模型的建立[J].第四軍醫大學學報,2003; 24(2):189.
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10.	趙振林,郭永章,李立,等.T細胞疫苗誘導免疫耐受與外周血T細胞凋亡的關系探討[J].中國普外基礎與臨床雜志,2003; 10(4):359.
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12.	Newell KA,He G,Hart J,et al.Treatment with either anti-CD4 or anti-CD8 monoclonal antibodies blocks alphabeta T cell-mediated rejection of intestinal allografts in mice[J].Transplantation,1997; 64(7):959.
13.	Krieger NR,Yin DP,Fathman CG.CD4+ but not CD8+ cells are essential for allorejection[J].J Exp Med,1996; 184(5):2013.
14.	He G,Hart J,Kim OS,et al.The role of CD8 and CD4 T cells in intestinal allograft rejection:a comparison of monoclonal antibody-treated and knockout mice[J].Transplantation,1999; 67(1):131.
15.	Takeuchi M,Alard P,Streilein JW.TGF-beta promotes immune deviation by altering accessory signals of antigen-presenting cells[J].J Immunol,1998; 160(4):1589.
16.	Rastellini C,Lu L,Ricordi C,et al.Granulocyte/macrophage colony-stimulating factor-stimulated hepatic dendritic cell progenitors prolong pancreatic islet allograft survival[J].Transplantation,1995; 60(11):1366.
17.	Itoh M,Takahashi T,Sakaguchi N,et al.Thymus and autoimmunity:production of CD25+CD4+ naturally anergic and suppressive T cells as a key function of the thymus in maintaining immunologic self-tolerance[J].J Immunol,1999; 162(9):5317.
18.	Hsu FJ,Benike C,Fagnoni F,et al.Vaccination of patients with B-cell lymphoma using autologous antigen-pulsed dendritic cells[J].Nat Med,1996; 2(1):52.
19.	姜楠,李立.轉化生長因子β在移植排斥中的作用[J].中國普外基礎與臨床雜志,2003; 10(2):179.
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1. Grant D.Current results of intestinal transplantation.The International Intestinal Transplant Registry[J].Lancet,1996; 347(9018):1801.
2. Zhai Y,Kupiec-Weglinski JW.What is the role of regulatory T cells in transplantation tolerance?[J].Curr Opin Immunol,1999; 11(5):497.
3. Fairchild PJ,Waldmann H.Dendritic cells and prospects for transplantation tolerance[J].Curr Opin Immunol,2000; 12(5):528.
4. Hackstein H,Morelli AE,Thomson AW.Designer dendritic cells for tolerance induction:guided not misguided missiles[J].Trends Immunol,2001; 22(8):437.
5. Dhodapkar MV,Steinman RM,Krasovsky J,et al.Antigen-specific inhibition of effector T cell function in humans after injection of immature dendritic cells[J].J Exp Med,2001; 193(2):233.
6. 李紀鵬,黃怡,王為忠,等.TGF-1基因轉染對大鼠樹突狀細胞生物學特性的影響[J].山東醫藥,2007; 47(24):23.
7. 董光龍,李開宗,王為忠,等.雙Cuff雙造口大鼠異位小腸移植模型的建立[J].第四軍醫大學學報,2003; 24(2):189.
8. Toogood GJ,Rankin AM,Tam PK,et al.The immune response following small bowel transplantation.Ⅱ.A very early cytokine response in the gut-associated lymphoid tissue[J].Transplantation,1997; 63(8):1118.
9. Kezuka T,Streilein JW.Analysis of in vivo regulatory properties of T cells activated in vitro by TGF beta 2-treated antigen presenting cells[J].Invest Ophthalmol Vis Sci,2000; 41(18):1410.
10. 趙振林,郭永章,李立,等.T細胞疫苗誘導免疫耐受與外周血T細胞凋亡的關系探討[J].中國普外基礎與臨床雜志,2003; 10(4):359.
11. 譚廣,任金帥,王忠裕,等.TNF-α增強樹突狀細胞誘導的抗胰腺癌免疫應答的實驗研究[J].中國普外基礎與臨床雜志,2006; 13(6):689.
12. Newell KA,He G,Hart J,et al.Treatment with either anti-CD4 or anti-CD8 monoclonal antibodies blocks alphabeta T cell-mediated rejection of intestinal allografts in mice[J].Transplantation,1997; 64(7):959.
13. Krieger NR,Yin DP,Fathman CG.CD4+ but not CD8+ cells are essential for allorejection[J].J Exp Med,1996; 184(5):2013.
14. He G,Hart J,Kim OS,et al.The role of CD8 and CD4 T cells in intestinal allograft rejection:a comparison of monoclonal antibody-treated and knockout mice[J].Transplantation,1999; 67(1):131.
15. Takeuchi M,Alard P,Streilein JW.TGF-beta promotes immune deviation by altering accessory signals of antigen-presenting cells[J].J Immunol,1998; 160(4):1589.
16. Rastellini C,Lu L,Ricordi C,et al.Granulocyte/macrophage colony-stimulating factor-stimulated hepatic dendritic cell progenitors prolong pancreatic islet allograft survival[J].Transplantation,1995; 60(11):1366.
17. Itoh M,Takahashi T,Sakaguchi N,et al.Thymus and autoimmunity:production of CD25+CD4+ naturally anergic and suppressive T cells as a key function of the thymus in maintaining immunologic self-tolerance[J].J Immunol,1999; 162(9):5317.
18. Hsu FJ,Benike C,Fagnoni F,et al.Vaccination of patients with B-cell lymphoma using autologous antigen-pulsed dendritic cells[J].Nat Med,1996; 2(1):52.
19. 姜楠,李立.轉化生長因子β在移植排斥中的作用[J].中國普外基礎與臨床雜志,2003; 10(2):179.
20. Aziz T,Hasleton P,Hann AW,et al.Transforming growth factor beta in relation to cardiac allograft vasculopathy after heart transplantation[J].J Thorac Cardiovasc Surg,2000; 119(4 Pt 1):700.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Immature Dendritic Cell Transfected by TGF-β1 Recombinated Adenovirus Vector on T Lymphocyte in Peripheral Blood and Allograft af ter Small Bowel Transplantation in Rat

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