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        west china medical publishers
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        find Author "廖秀清" 3 results
        • 聯合檢測微衛星不穩定性和p16甲基化在早期肺癌中的診斷價值

          目的 研究聯合檢測微衛星不穩定性( MSI) 和p16 基因啟動子甲基化在早期肺癌中的診斷價值。方法 對肺癌、癌前病變和正常肺組織采用PCR 單鏈長度分析法檢測MSI, 采用甲基化特異PCR 法檢測p16 甲基化。結果 癌前病變組MSI 的發生率顯著高于肺癌組( P lt;0. 05) 和正常肺組織組( P lt;0. 01) , 肺癌組MSI 的發生率顯著高于正常肺組織組( P lt;0. 01) ; 肺癌組p16 甲基化的發生率顯著高于癌前病變組( P lt;0. 01) 和正常肺組織組( P lt;0. 01) , 癌前病變組p16 甲基化的發生率顯著高于正常肺組織組( P lt;0. 01) ; 聯合檢測MSI 和p16 甲基化的敏感性顯著高于單一檢測MSI( P lt;0. 01) 和p16 甲基化( P lt;0. 05) ; 聯合檢測法與單一檢測MSI 和單一檢測p16 甲基化的特異性比較,差異無顯著性意義( P gt;0. 05) 。結論 聯合檢測MSI 和p16 甲基化可以顯著提高早期肺癌診斷的敏感性同時不降低其特異性, 值得臨床推廣。

          Release date:2016-08-30 11:52 Export PDF Favorites Scan
        • 慢性阻塞性肺疾病患者血漿網膜素-1 水平與病情嚴重程度的相關性研究

          目的研究血漿網膜素-1 在慢性阻塞性肺疾病(簡稱慢阻肺)患者臨床評價中的價值,并進一步探討其與慢阻肺急性加重病情嚴重程度的相關性。方法選取 124 例慢阻肺患者,按照病程分為急性加重組(64 例)與穩定期組(60 例),另選取 60 例無慢阻肺的健康成人作為對照組。急性加重組按照病情嚴重程度進行臨床分級。檢測研究對象的血漿網膜素-1 水平;分析慢阻肺患者血漿網膜素-1 水平與第 1 秒用力呼氣容積占預計值百分比(FEV1%pred)、用力肺活量占預計值百分比(FVC%pred)、GOLD 分級、改良呼吸困難指數(mMRC)評分、急性加重風險評估分組、氧合指數(PaO2/FiO2)、動脈血二氧化碳分壓(PaCO2)、慢阻肺急性加重臨床分級等指標的相關性。結果慢阻肺患者的血漿網膜素-1 水平明顯低于對照組(P<0.05),其中慢阻肺急性加重組的血漿網膜素-1 水平較穩定期組更低(P<0.01)。慢阻肺患者的血漿網膜素-1 水平與 FEV1%pred、FVC%pred 呈正相關,與 GOLD 分級、mMRC 評分、CAT 評分呈負相關(P<0.01)。慢阻肺急性加重患者血漿網膜素-1 水平與 PaO2/FiO2 呈正相關,與 PaCO2、呼吸衰竭類型、中性粒細胞百分比、住院天數、機械通氣時間呈顯著負相關(P<0.05)。隨著 GOLD 分級的增加,慢阻肺急性加重患者血漿網膜素-1 水平進行性降低,在 ABCD 綜合評估中臨床癥狀越重者其血漿網膜素-1 水平越低。慢阻肺急性加重患者的血漿網膜素-1 水平與臨床分級與呈顯著負相關(P<0.01)。血漿網膜素-1 水平在慢阻肺急性加重不同臨床分級之間有顯著差異(P<0.05)。與入院第 1 天相比,慢阻肺急性加重患者出院前 1 天的血漿網膜素-1 水平明顯增高(P<0.01)。結論血漿網膜素-1 與慢阻肺臨床評價指標具有一定相關性,可作為評估急性加重的生物學標志物。

          Release date:2020-09-27 06:38 Export PDF Favorites Scan
        • Investigation of Cognition Degree and Clinical Use of 2011 GOLD New COPD Classification System in Respiratory Specialist from Chongqing

          Objective To investigate the cognition degree and clinical use of new COPD classification system of 2011 GOLD in respiratory specialists, and further analyze the reasons of failing to clinical use. Methods Respiratory specialists from 42 hospitals in Chongqing were investigated through questionnaire survey. The questionnaire contains two parts. The first part contains nine questions about the knowledge of 2011 GOLD new COPD classification system and its clinical use. The second part contains six questions about the reasons of failing to clinical use of the COPD classification system. Results A total of 204 valid questionnaires were recovered. More than 90% respiratory specialists had understood the new COPD classification system with different degree, and believed it is suitable for clinical use. More than twothirds respiratory specialists knew well the ways about CAT and mMRC, but only 24% specialists were using these ways. The main reasons of failing to clinical use were as follows: 60% specialists believed the pulmonary function test can evaluate the COPD classification, and 66. 7% specialists were limited by short visit time. The cognition degree and clinical use of the new COPD classification systemin the specialists from third grade A class hospitals was better than those from the other hospitals. But the difference was not significant among specialists with different professional title.Conclusion Respiratory specialists in Chongqing knew well about the new COPD classification systemin 2011 GOLD, but did not use it widely in clinical works due to the complicated operation of the new COPD classification system.

          Release date:2016-09-13 03:53 Export PDF Favorites Scan
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