Single cell RNA sequencing technique provides a strong technical support for the analysis of cell heterogeneity in biological tissues, and has been widely used in biomedical research. In recent years, considerable scRNA-seq data have been accumulated in the research of ocular fundus diseases. The ocular fundus is abundant for the network of vessel and neuron, which leads to the complicated pathogenesis of fundus diseases. Through single cell RNA sequencing technique, the expression of thousands of genes of certain cell types or even subtypes can be obtained in the disease environment. Single cell RNA sequencing technique accurately reveals the pathogenic cell types and pathogenic mechanisms of ocular fundus diseases such as neovascular retinopathy, which provides a theoretical basis for the birth of new diagnosis and treatment targets. The construction of multi-omics single-cell database of ocular fundus diseases will enable high-quality data to be further explored and provide an analysis platform for ophthalmic researchers.
Objective
To evaluate changes of local flow field following implantation of a designed iliac venous stent.
Methods
Anin vitro physical model with iliocava junction was established. A modified iliac venous stent was implanted into one side and a conventional stent into the other side. The bilateral local flow fileds following implantation of stents were measured by using particle image velocimetry.
Results
The results of particle image velocimetry testing revealed that the main flow orientation was shifted to the right side and the region of slowly local flow was observed after implantation of conventional iliac venous stent. However, no evident changes were noted in the local flow field and no whirling flow was detected following implantation of the designed iliac venous stent.
Conclusion
Conventional stent is implanted into iliac vein by using a traditional technique and stent is proximal to heart and covers opening of contralateral iliac vein, which exerts certain influence upon local flow field. Local flow field has no obvious change following implantation of a designed iliac venous stent.
