Objective To systematically evaluate the correlation between the expression of microRNA (miRNA)-21 and the prognosis of esophageal cancer. Methods PubMed, Cochrane Library, Embase, Wanfang Data, China National Knowledge Infrastructure and VIP Databases were searched by for the literature on the correlation between miRNA-21 and the prognosis of esophageal cancer till July 10, 2022. Two researchers independently performed literature screening, quality evaluation, and data extraction. Statistical analysis was conducted with Stata 14.0. Results A total of 13 articles were included, including 1 204 patients. The results of meta-analysis showed that: the overall survival (OS) of patients with high expression of miRNA-21 was lower than that of patients with low expression of miRNA-21 [hazard ratio (HR)=2.11, 95% confidence interval (CI) (1.56, 2.84), P<0.001]. miRNA-21 expression was not associated with disease free survival [HR=2.53, 95%CI (0.67, 8.22), P=0.182]. The OS of Asian patients with high expression of miRNA-21 was significantly lower [HR=2.44, 95%CI (1.71, 3.49), P=0.005], while the OS of non-Asian patients was not related to miRNA-21 expression [HR=1.34, 95%CI (0.94, 1.91), P=0.363]. The high expression of miRNA-21 was correlated with the decreased OS in patients with esophageal squamous cell carcinoma [HR=2.22, 95%CI (1.52, 3.26), P=0.001], while the OS in patients with esophageal adenocarcinoma was not correlated with the expression of miRNA-21 [HR=1.39, 95%CI (0.63, 3.06), P=0.409]. Conclusion The overexpression of miRNA-21 is associated with poor prognosis and might be regarded as a potential prognostic biomarker for patients with esophageal cancer.
ObjectiveTo explore the predictive value of microRNA-215 (miR-215) and microRNA-7-5p (miR-7-5p) expression levels in gastric cancer tissues for recurrence after endoscopic submucosal dissection (ESD) based on restricted cubic spline (RCS). MethodsA total of 200 patients with early gastric cancer who underwent ESD in Yibin Second People’s Hospital from August 2020 to August 2022 were prospectively selected. The patients were followed up for 2 years, and the postoperative recurrence was counted. RCS was used to analyze the predictive value of miR-215 and miR-7-5p expression levels in gastric cancer tissues for recurrence after ESD and the dose-response relationship with recurrence after ESD. Helicobacter pylori (HP) infection, tumor maximum diameter, depth of invasion, carbohydrate antigen 724 (CA724), and neutrophils to lymphocytes ratio (NLR) levels were included to construct a basic nomogram prediction model, and miR-215, miR-7-5p, HP infection, tumor maximum diameter, depth of invasion, CA724, and NLR were included to construct a new nomogram prediction model. The value of the two models in predicting recurrence after ESD was compared. The clinical data of 84 patients with early gastric cancer who underwent ESD in Deyang People’s Hospital from September 2022 to September 2023 were retrospectively collected for external validation. ResultsDuring the follow-up period, 5 of 200 patients with early gastric cancer were lost to follow-up, and 195 patients were included and analyzed. The recurrence rate after ESD was 11.28% (22/195). The expression level of miR-215 in gastric cancer tissues of recurrence group and non-recurrence group [recurrence group: (0.67±0.15) vs. (0.97±0.24); non-recurrence group: (0.84±0.19) vs. (0.98±0.26)], miR-7-5p [recurrence group: (0.45±0.12) vs. (1.59±0.36); non-recurrence group:(0.68±0.20) vs. (1.61±0.37)] were lower than those in adjacent tissues (P<0.05). The expression level of non-recurrence group: (0.68±0.20) vs. (1.61±0.37)] was lower than that of adjacent tissues (P<0.05). The expression levels of miR-215 (OR=0.850, P=0.008) and miR-7-5p (OR=0.798, P=0.005) in gastric cancer tissues were the influencing factors of recurrence after ESD. There was a linear dose-response relationship between the expression levels of miR-215 and miR-7-5p in gastric cancer tissues and the recurrence after ESD (Poverall=0.009, Poverall<0.001; Pnonlinear=0.923, Pnonlinear=0.786). The area under curve (AUC) of the new nomogram prediction model including miR-215, miR-7-5p, HP infection, tumor maximum diameter, depth of invasion, CA724 and NLR for predicting recurrence after ESD was 0.964 [95%CI (0.900, 0.981)], which was greater than 0.857 [95%CI (0.796, 0.892)] of the basic nomogram prediction model, Z=2.158, P=0.032. The new nomogram prediction model has a high degree of calibration in predicting recurrence after ESD, and the clinical effect was good. External validation showed that the AUC of the new nomogram prediction model for predicting recurrence after ESD was 0.945 [95%CI (0.886, 0.978)], and the calibration degree for predicting recurrence after ESD was high and the clinical utility was good. ConclusionsThe expression levels of miR-215 and miR-7-5p in gastric cancer tissues of patients with early gastric cancer are lower than those in adjacent tissues, and there is a linear dose-response relationship with recurrence after ESD. The nomogram prediction model based on the expression levels of miR-215 and miR-7-5p in gastric cancer tissues can provide a reliable clinical basis for clinical prediction of postoperative recurrence.
Objective To explore the effect of long non-coding RNA H19 (lncRNA H19) on chronic heart failure (CHF) rats and its possible mechanism. Methods CHF (SD male rats, with a weight of 300±10 g, 10 weeks old) rat model was established by abdominal aortic coarctation. The 84 rats successfully modeled were randomly divided into a model group, a si-NC group [transfected lncRNA H19 small interfering RNA (siRNA) negative control], a si-H19 group (transfected lncRNA H19 siRNA), a si-miR-NC group [transfected microRNA-214 (miR-214) siRNA negative control], a si-miR-214 group (transfected miR-214 siRNA), a si-H19+si-miR-NC group (co-transfected lncRNA H19 siRNA and miR-214 siRNA negative control), and a si-H19+si-miR-214 group (co-transfected lncRNA H19 siRNA and miR-214 siRNA), 12 rats in each group. Another 12 rats were set up in a sham operation group (rats were only threaded without ligation, and the other operations were the same as the model group). After 4 weeks of intervention, the cardiac function, serum myocardial injury markers, heart failure markers, inflammatory related factors, apoptosis related factors and myocardial histopathological changes were compared. The expressions of lncRNA H19 and miR-214 in myocardial tissue were detected by real-time fluorescence quantitative PCR, and the targeting relationship between lncRNA H19 and miR-214 was detected by double luciferase reporter gene. Results Compared with those in the sham operation group, the myocardium of rats in the model group was severely damaged and a large number of inflammatory cells infiltrated; the lncRNA H19, cardiac function indexes (left ventricular end systolic diameter, left ventricular end diastolic diameter), serum myocardial injury markers (creatine kinase MB, cardiac troponin I), heart failure markers (brain natriuretic peptide, N-terminal pro brain natriuretic peptide), inflammatory related factors (interleukin-1β, interleukin-18, tumor necrosis factor-α, interleukin-6), cardiomyocyte apoptosis rate, apoptosis related proteins [B lymphocytoma-2 (Bcl-2), Bcl-2 related X protein (Bax), cysteinyl aspartate specific proteinase-1 (Caspase-1)] in the myocardial tissue of the model group were significantly increased (P<0.05); miR-214 of myocardial tissue, cardiac function indexes (left ventricular ejection fraction, left ventricular fractional shortening) and Bcl-2/Bax ratio were significantly decreased (P<0.05). Compared with the model group, silencing lncRNA H19 could significantly improve the cardiac function and the changes of the above indexes in CHF rats, and reduce myocardial injury (P<0.05); down-regulation of miR-214 could significantly reverse the protective effect of si-H19 on myocardial injury in CHF rats (P<0.05). Conclusion Silencing lncRNA H19 can up-regulate the expression of miR-214, inhibit the expression of Caspase-1, inhibit the apoptosis and inflammatory reaction of cardiomyocytes, and alleviate myocardial injury in rats with CHF.
