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Establishment of AdriamycinResistance Cell Substrain SMMC7721/Adriamycin of Human Hepatocellular Carcinoma and Research of Its Characteristics

ObjectiveTo establish multidrugresistance cell substrain of human hepatocellular carcinoma and to investigate its characteristics.MethodsSMMC7721 cell strain was cultured in Adriamycin(ADM). The multidrugresistance cell substrain SMMC7721/ADM was harvested after a long period of culture by gradually increasing the concentration of ADM and its characteristics were investigated. Results①The drug resistance of SMMC7721/ADM to ADM increased by 33.3 times, to Vincristine 16.8 times, to Diamminedichloroplatinum 2.8 times. ②The drug resistance cell substrain had almost the same growth velocity as its parental generation. The doubling time was 32.0 hours and 30.5 hours respectively. They had the analogous growth curves. ③The obvious difference between the drug resistance cell substrain and its parental generation was that the former’s microvilli became thick, short and scattered by scanning and transmitting electron microscopy. ④The multidrug resistance cell substrain kept the characteristics of hepatocellular carcinoma, it could be transplanted into the subcutaneous tissue of nude mice. ⑤The drug resistance of the cell substrain reduced to 28.0% and 9.2%after removal of the drug for 1 month and 2 months respectively, its drug resistance could remain stable (35.4 times) after 2 months of culture in ADM (0.04 μg/ml).ConclusionThe SMMC7721/ADM cell substrain has the stable fundamental characteristics of a drug resistance cell strain.

Analysis of Health Examination Results in 1 089 Retirees

ObjectiveTo analyze the health examination results of hospital retirees, understand their health status and provide the evidence for health management. MethodsThe data were collected from our 1 089 hospital retirees (51-96 years old) who received health examination in West China Hospital of Sichuan University from January to December 2013, including 345 males and 744 females, with a mean age of 70 years. The data were analyzed by SPSS 16.0 software. ResultsHypertension, dyslipidemia and diabetes were the three chronic diseases with the highest detectable rate, and the rate was respectively 49.49%, 44.90% and 31.04%. The detectable rate of hypertension was not significantly different between male and female. The rate of dyslipidemia in females was higher than that in males. The rate of diabetes in males was higher than that in females. The detectable rate of hypertension and diabetes increased with the increase of age. The detectable rate of dyslipidemia had no significant relationship with age. ConclusionThe health condition of retirees cannot be neglected. We need to strengthen the health management for the retirees.

繼發于乳腺癌的雙眼表現不同的眼內轉移癌1例

醫務人員銳器傷現狀及精細化管理在防范銳器傷中的作用

銳器傷為醫務人員職業傷害的常見類型，其主要危害為傳播相關血源性疾病。該文綜述了國內外醫務人員發生銳器傷的現狀，分析了銳器傷發生的危險因素，并分析了對相關危險因素進行精細化管理后銳器傷的發生率變化情況，認為對銳器傷危險因素進行精細化管理可以有效降低銳器傷的發生率，減少血源性疾病傳播的概率，為銳器傷的防護工作提供參考。