【摘要】 目的 探討抗氧化應激是否參與參附注射液預處理誘導的腎臟保護作用。 方法 健康成年雄性SD大鼠21只隨機分為假手術對照組（Sham組）、腎臟缺血再灌注組（I/R組）和參附注射液組（SF組）；SF組給予參附注射液10 mL/kg腹腔注射，每日1次，連續給藥7d。麻醉下行右腎切除后，用無損傷動脈夾鉗夾左側腎蒂60min，再灌注24 h，制備腎缺血再灌注損傷動物模型。比較各組SD大鼠再灌注24 h腎臟組織中超氧化物歧化酶（superonidedismutase，SOD）水平、過氧化氫酶（catalese，CAT）和丙二醛（malonicalaldehyed，MDA）含量。 結果 與Sham組相比，I/R和SF組腎臟組織SOD和CAT顯著降低，而MDA明顯升高（Plt;0.05）；與I/R組比，參附注射液能明顯增加SOD和CAT水平（Plt;0.05），降低MDA含量（Plt;0.05）。 結論 參附注射液預處理可增強缺血再灌注損傷腎臟組織抗氧化應激，其表現為增強SOD和CAT的活力，減少MDA的生成。【Abstract】 Objective To explore the protective effect of Shenfu injection combined with antioxidant system on rats’ kidney after ischemia-reperfusion injury. Methods Twenty-one male Sprague Dawley (SD) rats were randomly divided into 3 groups: sham operation group (Sham group), ischemia-reperfusion group (IR group), and shenfu injection treated group (SF group). The rats were anesthetized with valebarbitone. Bilateral kidneys were exposed through midline incision. The right kidney underwent the nephrectomy and left renal pedicels were occluded for 60 minutes with a traumatic mini-clamp and then unclamped for 24 hours. Animals in SF group received Shenfu injection (10 mL/kg) through intraperitoneal injection every day for 7 days. About 24 hours after reperfusion, superoxide dismutase (SOD), CAT and malonical aldehyde (MDA) were measured. Results The levels of MDA were lower in SF group than those in IR group (Plt;0.05). The level of SOD and CAT in SF group increased more significantly than which did in IR group (Plt;0.05). Conclusion Our finding suggests that antioxidant system in SF group works more efficiently than IR group to overcome oxidative stress in renal ischemia-reperfusion injury.
目的建立離體人睪丸的缺血再灌注（I/R）損傷模型，為研究抗I/R損傷藥物的作用及機理提供一實質器官灌注模型。方法采用13例捐贈尸睪，用250 ml 0℃~4℃高滲枸櫞酸鹽嘌呤液灌洗后冷存，再以500 ml 37℃該液進行再灌注，不同時間點取材作組織學及酶組織化學檢查。結果4℃冷缺血12 h開始出現血管內皮細胞腫脹、變圓、空泡樣變，24 h內皮細胞變性脫落，睪丸曲細精管基膜與生精上皮剝離，生精細胞變性脫落，間質水腫等，病變隨冷缺血時間延長而加重。酶組化結果顯示，單純冷保存18 h后，睪丸組織乳酸脫氫酶（LDH）活性升高，琥珀酸脫氫酶（SDH）活性24 h后升高，經37℃復溫再灌注后損傷明顯加重。結論離體人睪丸可替代人體其它實質器官作為研究I/R損傷的離體灌注模型。
