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內因性色素膜炎37例的血清及房水弓形體抗體檢查

本文對37例內因性色素膜炎患者的血清、房水進行了弓形體抗體檢測，血清抗體陽性者9例，表明有弓形體感染者占24.3%。血清及房水均為陽性者5例，其中2例房水抗體滴度與血清抗體滴度的比例ge;4，結合典型的臨床表現確診為弓形體色素膜炎，占本組色素膜炎患者的5.41%。本組病例檢查說明弓形體感染為鄂西內因性色素膜炎的病因之一，血清、房水檢測對弓形體色素膜炎的診斷有一定價值。 （中華眼底病雜志，1992，8：145-147）

CLINICAL STUDY ON SERUM COMPLEMENT-3 AND PLASMA FIBRONECTIN OF PATIENTS WITH OBSTRUCTIVE JAUNDICE PRE- AND POST-OPERATION

Thirty patients with obstructive jaundice were investigated for serum complement-3 (C3) and plasma fibronectin (FN).The levels of C3 and FN of the juandiced patients were higher than that of thirty patients without obstructive jaundice (Ｐ＜0.01). As compared to pre-operation, the level of C3 of the jaundiced patients decreased obviously within two weeks after operation(Ｐ＜0.01), and recovered in the third week after operation. The level of FN of the juandice patients decreased evidently within one week(Ｐ＜0.01), and recovered in the second week after operation. However, the levels of C3 and FN of the patients without obstructive jaundice changed slightly after operation (Ｐ＜0.05). The high levels of C3 and FN of jaundiced patients may be relative to the latent infection. Consumption and immune imparing may be the reasons of C3 and FN to decrease.

The Relationship of Serum Calcium and Pancreatic Necrosis

目的:探討血清鈣與胰腺壞死的關系.方法:四川大學華西醫院中西醫結合科2003年9月~2005年3月收治的200例急性胰腺炎患者,根據CT結果分為壞死組和非壞死組,根據所有患者測得的血清鈣相應的真陽性率和假陽性率做出ROC曲線以預測胰腺壞死。結果:ROC曲線下面積為0.436。結論:血清鈣高低不能預測胰腺壞死。

The Clinic Value of Prealbumin, Cholinesterase and Total Bile Acid in Evaluating Liver Reserve Function in Patients with Liver Cirrhosis

摘要:目的: 探討血清前白蛋白(prealbumin,PAB)、膽堿脂酶(cholinesterase,ChE)、總膽汁酸(total bile acid,TBA)在肝硬化中的檢測價值。 方法 :測定105例肝硬化患者和30例健康人的前白蛋白、膽堿脂酶、總膽汁酸活性及肝功能生化指標,并按ChildPugh分級進行比較。 結果 :肝硬化組前白蛋白含量、膽堿脂酶活性均較對照組顯著降低;按ChildPugh分級比較,肝硬化組前白蛋白含量在Child A級與對照組、B級與A級之間、在C級與B級之間差異有顯著性(Plt;001);膽堿脂酶活性在Child A級與對照組、B級與A級、C級與B級之間差異有顯著性(Plt;001)。總膽汁酸在Child B級與A級,C級與B級間有顯著差異性,在A級與對照組間差異無顯著性。 結論 :血清前白蛋白、膽堿脂酶在肝硬化早期評估中有很重要的價值,而總膽汁酸在肝硬化預后的判定中有重要價值。 Abstract: Objective: To evaluate the role of prealbumin (PAB), cholinesterase (ChE), and total bile acid (TBA) in evaluating liver reserve function in patients with liver cirrhosis. Methods : One hundred and five serum samples from patients with liver cirrhosis were detected in PAB, ChE, TBA and other biochemical markers. All patients were classified in accordance with ChildPugh scale. Results : For PAB, the differences among ChildPugh A, B, C and healthy group were statistically significant (t=1254, 1887, 2316) (Plt;001). For ChE, the differences among ChildPugh A, B, C and healthy group were statistically significant (t=1288, 0856, 1002) (Plt;001). For TBA, the differences among ChildPugh C group, B group and A group were statistically significant (t=0526, 1081)(Plt;001), the difference among ChildPugh A group and healthy group was not statistically significant (t=5615) (Pgt;005). Conclusion : PAB and ChE reflect liver reserve function earlier in patients with liver cirrhosis. The role of TBA is important in reflecting prognosis in patients with liver cirrhosis.

玻璃體切割聯合自體血清治療黃斑裂孔性視網膜脫離

布氏桿菌病防治分析

【摘要】 目的 探討對布氏桿菌病的防治策略,減少對布氏桿菌病的誤診、誤治。方法 2007年—2009年,對45例布氏桿菌病患者誤診原因進行調查分析。結果 養畜戶、臨床醫生對布氏桿菌病認識不足及認識不夠將會延誤病情,加重其傳播流行。結論 普及布氏桿菌病防治知識,加強對此病的監督力度,有助于提高對布氏桿菌病的防治水平。

Relationship between the Level of Cholinesterase and the Patients Condition and the Prognosis of Sepsis

【摘要】　目的　探討膿毒血癥患者膽堿酯酶水平與患者病情及預后的關系。　方法　2007年6月-2009年6月，將89例膿毒血癥患者設定為膿毒血癥組，進行血清膽堿酯酶測定及APACHEⅡ評分；另擇82例健康人為正常組，測定血清膽堿酯酶值，比較兩者之間差異；89例膿毒癥患者按病況再分為存活組及死亡組，比較兩者之間血清膽堿酯酶及APACHEⅡ評分差異。　結果　治療前膿毒血癥組膽堿酯酶水平明顯低于正常組，有統計學意義（Plt;0.01）；膿毒血癥組APACHEⅡ評分與血清膽堿酯酶呈負相關；死亡組APACHEⅡ評分明顯高于存活組，而血清膽堿酶低于存活組（Plt;0.01）。　結論　膽堿酯酶同APACHEⅡ評分呈負相關，能明顯反映膿毒癥患者病情嚴重程度及預后。【Abstract】　Objective　To explore the relationship between the level of cholinesterase and the patients condition and the prognosis in the patients with sepsis.　Methods　From June 2007 to June 2009, 89 patients with sepsis were selected as the sepsis group, whose cholinesterase level was assayed and evaluated by APACHE Ⅱ score. Another 82 healthy people were as the control whose cholinesterase level was assayed and compared with that in the sepsis group. The patients in the sepsis group were subdivided into survival and death group; the level of cholinesterase and the result of APACHE Ⅱ score were compared between the two groups.　Results　The level of cholinesterase in sepsis group was significantly lower than that in the control group before treatment, and the difference was significant (Plt;0.01); the APACHE Ⅱ score negatively correlated with the serum cholinesterase in sepsis group. The APACHE Ⅱ score in the death group was significantly higher than that in the survival group, but the level of cholinesterase was obviously lower in the death group than that in the survival group (Plt;0.01).　Conclusion　The serumal cholinesterase negatively correlates with the APACHE Ⅱ score, which could obviously reflect the patients condition and the prognosis of sepsis.

EXPERIMENTAL STUDY ON ULTRA-FILTRATION IN REDUCING MATRIX EFFECTS OF RECOMBINATIONHUMAN ACELLULAR DERMAL MATRIX IMMERSION ON RESIDUAL BOVINE SERUM ALBUMINMEASUREMENT BY ELISA

Objective To investigate the effect of ultra-filtration on reducing the matrix effects of the immersionof recombination human acellular dermal matrix (rhADM) on detecting residual bovine serum albumin (BSA) by ELISA.Methods Preparation of rhADM immersion: rhADM were rinsed, and then rhADM immersion were prepared. Physiologicalsal ine was used as immersion medium. Presaturation and ultra-filtration: marked the ultra-filtration tubes as PR1 (presaturation protocol 1), PR2 (presaturation protocol 2) and rhADM, respectively, added 2 mL of 1 mg/mL and 10 μg/mL BSA solution into PR1 and PR2 respectively, and added 2 mL of rhADM immersion into rhADM tubes (rhADM1 and rhADM2). The tubes were then centrifuged at 1 500 × g for 20 minutes. The above steps were repeated for 3 times. Take the inner-tube of ultrafiltration into unused centrifuge tube. Added 4 mL of 10 μg/mL BSA solution in PR1 and PR2 tubes, 4 mL of rhADM immersion in rhADM tubes, centrifuged at 1 500 × g for 20 minutes, and then the filtration was colleted. Detecting BSA concentration: the BSA concentrations of all samples were detected by using the quantitative measure of residual BSA ELISA kit. The recoveries of 10 μg/ mL BSA solution treated by presaturation protocol 1 and 2 were calculated (untreated 10 μg/mL BSA solution was as the basic sample, marked R10 and R20 respectively). The correlation coefficient between the logarithm of the filtrate dilution and the absorbance (A) value was calculated and compared with that of water exact without ultra-filtration. Results The BSA concentration of PR1 and R10 was (23.80 ± 1.58) μg/ mL and (9.04 ± 0.24) μg/mL, respectively. The BSA concentration of PR2 and R20 was (8.64 ± 0.24) μg/mL and (8.12 ± 1.01) μg/ mL, respectively. The average recovery of 10 μg/mL BSA was 263.4% ± 16.9% and 106.5% ± 3.0% when the ultra-filtration tubes were presaturaed by PR1 and PR2 (P lt; 0.01), respectively. The BSA recovery of PR2 met the detecting demand. The correlations between A value and sample dilution were increased, the correlationcoefficient was raised from — 0.727 to — 0.960 after rhADM immersion were treated by ultra-filtration. Conclusion Theresults show that the matrix effects can be reduced effectively by ultra-filtration, indicating that an acceptable recovery of BSA can be acquired when ultra-filtration tube is presaturated by sample water extract.

Value of Serum Low Sodium Low Chlorine in the Diagnosis of Adult Intracranial Infection

目的:觀察顱內感染急性期血清鈉氯濃度的改變及其臨床意義。方法:對160例顱內感染血清鉀鈉氯濃度進行動態監測。結果:顱內感染組低鈉低氯發生率顯著高于對照組(P＜0.05),結腦患者最高、化腦次之、病腦患者最低,但均高于對照組。各組間血清鉀鈉氯水平差異無統計學意義(P＜0.05),但與對照組比較有顯著性意義差異(P＜0.05)。結論:成人顱內感染急性期大部分存在明顯的低鈉低氯血癥,且不同病原感染所致低鈉低氯的水平不相同,顱內感染低鈉低氯與患者病情輕重成正相關。故動態監測血清鉀鈉氯有助于顱內感染的診斷和鑒別診斷以及病情輕重和預后估計。

Effect of MultiSlice Spiral Computer Tomography Combined with Serum Amyloid A Protein on Preoperative Rectal Cancer Staging

摘要:目的: 探討64排多層螺旋CT(MSCT)和血清淀粉樣蛋白A(serum amyloid A protein, SAA)聯合術前評估直腸癌在腫瘤分期診斷中的作用。 方法 :納入經根治術治療的直腸癌患者通過MSCT掃描進行評估,同時取患者靜脈血測量術前SAA水平,行MSCT分期與MSCT和SAA聯合分期以比較二者的診斷價值。 結果 :本研究納入患者121例。MSCT檢測T分期的準確度為851%。在評估淋巴結轉移方面,MSCT和SAA聯合分期的準確度為760%,明顯高于MSCT分期(595%, 〖WTBX〗P lt;0001)。MSCT正確判斷所有遠處轉移。同單一的MSCT檢測相比,MSCT和SAA聯合評估能顯著的提高術前TNM分期的準確率(785% vs. 636%,〖WTBX〗P =0011)。 結論 :MSCT聯合SAA檢測比單一的MSCT檢測顯著提高了直腸癌術前腫瘤分期和淋巴結轉移方面的準確度。這種新的術前評估方法的為腫瘤進展評估和術前治療決策提供了更加可靠的信息。Abstract: Objective: To determine the role of combinative assessment of 64 multislice spiral computer tomography (MSCT) and serum amyloid A protein (SAA) in preoperative rectal cancer staging. Methods : Enrolled consecutive rectal cancer patients undergoing curative surgery were evaluated by MSCT scan. Meanwhile venous blood specimens were taken to measure preoperative SAA concentration. Both MSCT staging and MSCT plus SAA staging were performed to compare with each other. Results : The study population consisted of 121 patients. The accuracy of T staging was 851% for MSCT. The accuracy in evaluating lymph nodes metastases was 760% for MSCT plus SAA compared with 595% for MSCT alone (〖WTBX〗P lt;0001). All the distant metastases were correctly detected by MSCT. The method combining MSCT with SAA led to significant improvement on preoperative TNM staging compared with MSCT alone (785% vs. 636%, 〖WTBX〗P =0011). Conclusion : MSCT plus SAA showed greater accuracy than MSCT alone in rectal cancer staging and lymph node metastases. This novel strategy of preoperative evaluation appears to provide more accurate information on tumor progression and preoperative therapy decisionmaking.