Objective
To observe the difference between blood brain barrier and blood optic nerve
barrier.
Methods
Twenty normal male SD rat sprime; optic nerve including prelaminar region, lamina
cribrosa, retro-laminar region, intraorbital portion, intracanalicular portion, and
intracranial portion respectively,and cerebral cortex were removed separately.
Ultrastructure of endothelial cells was observed by electron microscopy.
Immunohistochemical staining was used to detect the expression of transferrin
receptor (OX-26) and metalloproteinase inducer (OX-47) and extravasation of
fibrinogen around microvessels.
Results
The results of electron microscopy showed that endothelial cells of microvessels
in each portion of optic nerves and cerebral cortex did share the same tight
junctions. However, the number of plasmalemmal vesicles in prelaminar region
was significantly more than that in cerebral cortex(P＜0.05);there was no
significant difference between other parts of optic nerves (lamina cribrosa, retro- laminar region, intraorbital portion, intracanalicular portion, and intracranial
portion)and cerebral cortex in the number of the plasmalemmal vesicles(Pgt;0.05). By immunohistochemical staining，the endothelial cells of microvessels in the
prelaminar region showed no expression of the OX-26 and OX-47,but
extravasation of fibrinogen around microvessels was found; b positive
expression of OX-26 and OX-47 was observed in the endothelial cells of the
microvessels in other parts of optic nerves (lamina cribrosa, retro-laminar region, intraorbital portion, intracanalicular portion, and intracranial portion) andcerebral cortex, and no fibrinogen was seen aro und the microvessels.
Conclusions
There is a significant difference between the endothelial cells of the microvessels in prelaminar region and cerebral cortex in the ultrastructure, markers expression, and permeability, so the microvessel s in prelaminar region lacks the typical blood brain barrier characteristics.The microvessels in other parts of optic nerves (lamina cribrosa, retro-laminar region, intraorbital portion, intracanalicular portion, and intracranial portion) have blood brain barrier properties due to its similar specialties as which in cerebral cortex.
(Chin J Ocul Fundus Dis, 2006, 22: 390-393)
