【摘要】 目的 探討骨形成蛋白-2（bone morphogenetic protein-2，BMP-2）對室管膜前下區（anterior subventricular zone，SVZa）神經干細胞DLX5表達的影響。 方法 體外培養SVZa神經干細胞，用BMP-2及其拮抗劑Noggin誘導SVZa神經干細胞，分別用免疫熒光染色和逆轉錄-聚合酶鏈反應（RT-PCR）檢測DLX5表達變化。 結果 BMP-2組SVZa神經干細胞DLX5蛋白表達和DLX5mRNA表達水平明顯高于對照組（Plt;0.05），且該效應能被其拮抗劑Noggin特異性地抑制。 結論 BMP-2是DLX5上游調節基因，可促進SVZa神經干細胞DLX5的表達。【Abstract】 Objective To investigate the effect of bone morphogenetic protein-2 (BMP-2)on expression of DLX5 of neural stem cells in anterior subventricular zone (SVZa). Methods The neural stem cells of SVZa were separated and cultured in vitro, which were induced by BMP-2 and Noggin.Immunofluorescence staining and RT-PCR were employed to assay the expression of DLX5. Results The percentages of expression of DLX5 protein and DLX5 mRNA in BMP-2 group were much higher than those in the control group (Plt;0.05). And this induction could be specifically blocked by Noggin. Conclusion BMP-2 is an upstream gene of DLX5; BMP-2 can promote the expression of DLX5 of the neural stem cells of SVZa.
