Objective To investigate the effect of constitutively active Akt1 gene on rat engrafted islets in apoptosis and revascularization, and to explore potential method of gene therapy in the islet transplantation. Methods Rat islet which was transfected constitutively actived Akt1 gene via adenovirus vector using MOI=500. Thirty-six streptozotocin induced diabetic Wistar rats were divided into 3 groups complete randomly: Adv-CA-Akt1 group, Adv-LacZ group and simple transplantation group. Blood glucose and insulin were determined after operation. TUNEL was used to detect the apoptotic islet cells. HE and immunohistochemical staining of insulin were used to evaluate the histology of the islet grafts. The microvessel density (MVD) was determined by CD31 immunohistochemical staining. Results The fasting glucose level in Adv-CA-Akt1 group restored to normal 2 days after transplantation. However, in Adv-LacZ group and simple transplantation group, it reduced but still kept being hyperglycemia. And the serum insulin level was higher than other two groups ( P ＜ 0.05). Compared to simple transplantation group and Adv-LacZ group, apoptotic rate decreased 25% in Adv-CA-Akt1 group, a large number of islet grafts were seen under the capsule of the kidney, which were positively stained by insulin antibody. In the other two groups, the islet groups mass were lighter, and few positively stained by insulin antibody. MVD showed lighter positive endothelial cells stained by CD31 antibody in the other two groups than Adv-CA-Akt1 group ( P ＜ 0.05). Conclusion Constitutively activate Akt1 gene can prolong graft survival during early posttransplant period, and can accelerate the revascularization of islet grafts effectively.
Objective To learn and analyse the current clinicians’ knowledge and attitudes towards patient safety, and to provide relevant evidence for future medical education. Method We conducted a survey on clinicians mainly in West China Hospital of Sichuan University with group random sampling method. We analysed the data on the clinicians’ knowledge and attitudes with SPSS softerware. Result Totally 300 questionnaires were distributed, of which 258 were completed adequately. The results showed the clinicians’ knowledge on patient safety was poor, however, the respcnse from the clinicians in Outpatient Department were better than those in Inpatient Department. The majority of the clinicians (above 95.00%) were willing to learn the knowledge of patient safety. Conclusion As the clinicians are willing to learn the knowledge of patient safety positively, it is necessary to integrate patient safety education into the current medical education curriculum.
Objective To study the special traits of primiparae’s compliance with labor analgesia, so as to offer individualized analgesia solutions during spontaneous labor. Methods The uniparous primiparae with cephalic presentation between gestational weeks 38 and 40 were divided into two groups based on their educational background (college education or above, and high school education or below), each group with 20 cases. The demographical statistics of the two groups including their State-Trait Anxiety Inventory (STAI) grading, PCA results, and delivery situation were recorded and analyzed. Results Differences in age, height, and weight were not statisticallysignificant (Pgt;0.05); differences in T-AI were not statistically significant (Pgt;0.05); differences in S-AI were statistically significant (Plt;0.05); differences in anxiety and numbers of adding anesthetics were not statistically significant (Pgt;0.05); differences in failure to tolerate labor pains and requiring caesarean section were statistically significant (Plt;0.05). Conclusion Primiparae with higher educational degree tend to have higher S-AI grading and perform poorly in compliance with labor analgesia.
Objective To explore the application and effects of Seminar case teaching method in teaching surgical nursing. Methods Nursing students of the Yiyang Junior Medical College were divided into two groups (140 students/group). Seminar case teaching was applied in the trial group, while traditional teaching method (classroom teaching or instruction) was applied in the control group. The two groups used the same materials, and were taught by the same teacher team during the same course. After the courses, surgical knowledge and skills were evaluated using theory exams and operation tests (tools included communication ability scale as well as the California Critical Thinking Dispositions Inventory Scale for students’ ability and quality levels, and an anonymous questionnaire for teaching satisfaction) were analyzed between the two groups. Results The scores of the trial group were higher than those of the control group in theory exams and operation tests, with a significant difference (Plt;0.01). The before-after score differences of communication skills and critical thinking ability of the trial group were also higher than those of the control group, with a significant difference (Plt;0.01). The result of the anonymous questionnaire showed that, the students in the trial group had better satisfaction about Seminar case teaching method. Conclusion Seminar case teaching method applied in surgical nursing teaching is feasible and effective, which is better than traditional teaching method in improving knowledge, ability, and quality of nursing students.
ObjectiveTo explore the levels of serum glucose and insulin secretion function in people with different educational background of normal glucose tolerance and to investigate its relationship with educational levels.
MethodsWe had telephone connections with normal fast glucose people who did health examination from January 2007 to January 2011, then the 75 g oral glucose tolerance test was performed and the level of insulin were measured. A total of 340 people with normal glucose tolerance who completed our examination and answered a questionnaire on lifestyle were included in our study. They were divided into three groups according to their educational levels. The levels of plasma glucose and insulin were measured in 92 high educational level participants, 68 middle educational level participants and 70 low educational level participants. We calculated homevstasis model assessment (HOMA)-S, HOMA-IR, and HOMA-β and compared differences between the three groups.
ResultsWith the decline of educational levels, fasting plasma glucose, body mass index (BMI), fasting insulin, postprandial insulin, HOMA-β, and HOMA-IR gradually increased, while HOMA-S gradually decreased. The level of BMI, fasting insulin and HOMA-IR in high educational level group were significantly lower than those in low educational level group. The level of BMI, HOMA-IR in middle educational level group were significantly lower than those in low educational level group (P<0.05). Correlation analysis indicated that level of fasting plasma glucose was related to age, BMI, and educational levels.
ConclusionWith the decline of educational levels, BMI and HOMA-IR increase, and HOMA-S decreases, which indicates that they already have differences in glucose metabolism and insulin secretion function in people with normal glucose tolerance, presaging that in the future the proportion of development of diabetes in the low educational level may increase.
Since the appearance of the concept of evidence-based medicine in 1992, its advanced conception and scientific method has been widely applied in various social science fields, and several sub-disciplines were formed such as evidence-based management, evidence-based education, and evidence-based social work and so on. However, there has been no studies to review its history and condition until now. The purpose of this study was to introduce the evidence-based social science in terms of its origin, development conditions, challenges and strategies. We hope it can serve as reference for research and policy makings in the fields of social science in China.
Abstract： Objective To investigate the effect of intramyocardial injection of slow release microspheres of tannic acid （TA） on ventricular remodeling after acute myocardial infarction （AMI） in rats. Methods Slow release microspheres of TA were prepared and the release parameters in vitro were detected. AMI model in rats was induced. Eighty rats were enrolled and divided into 4 groups by random digital table：poly （lactic-co-glycolic acid） （PLGA） microspheres injection （PLGA group， n=24）， PLGA-TA microspheres injection （PLGA-TA group， n=24）， TA injection group （TA group， n=16） and normal saline injection group （NS group， n=16）. Heart function was evaluated by echocardiography after the injection. The structure of cardiac extracellular matrix （ECM） in the infarcted borderline area was evaluated at 4th week after the injection. Collagen content in the infarcted area was evaluated by hydroxyproline colorimetry assay at 2nd and 4th week after the injection. Results TA release was maintained at a constant rate from the microspheres for one month in vitro. Two weeks after the injection， left ventricular ejection fraction（LVEF）， left ventricular fraction shortening（LVFS）， left ventricular end-diastolic diameter（LVEDD） and left ventricular end-systolic diameter（LVESD） in PLGA-TA group and TA group were significantly better than those in the other two groups（P＜0.05）. Four weeks after the injection， LVEF， LVFS， LVEDD and LVESD in PLGA-TA group were significantly better than those in the other three groups （P＜0.05）. Four weeks after the injection， slow release microspheres of TA in the PLGA-TA group effectively improved the arrangement of ECM compared with TA group. Four weeks after the injection， collagen content in the infarcted area of PLGA-TA group was significantly higher than that in TA group（88.88±7.28 μg/mg dry weight vs. 72.43±9.02 μg/mg dry weight）， PLGA group（88.88±7.28 μg/mg drg weight vs. 71.97±6.06 μg/mg dry weight） and NS group（88.88±7.28 μg/mg dry weight vs. 68.86±7.55 μg/mg dry weight， F=7.162，P=0.003）， but there was no statistical difference in the collagen content of the infarcted area among TA group， PLGA group and NS group （P＞0.05） . Conclusion Intramyocardial injection of slow release microspheres of TA can maintain a constant release of TA for a comparatively long period， inhibit collagen matrix degradation， and effectively attenuate ventricular remodeling after AMI in rats.
" Inquiry-small class” teaching mode is a novel teaching method aimed at cultivating the clinical capability and professionalism and promoting comprehensive development of medical students. Small-class teaching mode prioritize students and promotes deep interaction between teaching and learning. Therefore, integrating small class teaching into large class teaching is essential. This paper attempts to explore the applications and prospects of " Inquiry-small class” teaching mode in clinical medicine education through summarizing teaching concept and design, learning resources, exam reform, teacher-student interaction and other aspects of the course " endocrine and metabolic disease (bilingual)”, offered by West China School of Medicine in Sichuan University, so as to provide more reference for the future " Inquiry-small class” teaching reform.
Objective To find the best teaching method of summer EBM course to help students understand and apply EBM principles in research. Methods Training medical students to participate in an international team of clinical research for best evidence. We used a step-by-step method to teach how to write Cochrane systematic review and apply its method to other researches. Results Since our training program was launched three years ago, 38 medical students have taken part in the training. Five Cochrane systematic reviews and 19 protocols were published in The Cochrane L ibrary. Three medical students were the principal investigators and played an important role in another 2 studies. Seven articles were published in Chinese medical key journals, and 1 was indexed by SCI. Eleven students were funded to participate in the international academic conferences.Conclusions Medical students will benefit from taking part in the EBM research. This activity will help them not only understand method of clinical research and EBM , improve the quality of research and their cooperative skills but also master related clinical knowledge.
Traditional medical education is to develop uni-professionals,and is lacking of opportunities for interprofessional communication and interprofessional collaboration.The failure of communication and collaboration is an important cause of medical errors.Interprofessional education is a new strategy to remove professional prejudice,promote interprofessional collaborative competency and improve patients'outcomes.From September 2012,we began to provide Interprofessional Education Program for healthcare undergraduates,and achievements have already been made.
