ObjectiveTo investigate the synergetic effects of the combination methylene blue or/and aprotinin on preventing postoperative intestinal adhesions (POIA).MethodsFourtyeight rabbits were divided into control group (group A), methylene blue group (group B), aprotinin group (group C), methylene blue+aprotinin group (group D). Each group contained 12 rabbits and established models of intestinal adhesions through laporotomy. Fourteen days after operation, the rabbits were reoperated to see whether there were adhesions and the degree of adhesions. ResultsThe adhesions of group A was the most serious, then in sequence were group B, C, group D showed very light adhesion. Group A was much more severe than B, C, D group (Plt;0.01); group D was very different from B, C group (P＜0.05). ConclusionMethylene blue and aprotinin have significant effects on preventing POIA and the combination of the two drugs can have a synergetic effects on POIA.
ObjectiveTo investigate the anti-inflammatory mechanism of sodium aescinate in preventing postoperative intestinal adhesion in rats.
MethodsThe SD rats were subjected to operation for establishing intestinal adhesion models, then randomly divided into model group, dexamethasone group(dexamethasone, i.v. 5 mg/kg), and sodium aescinate group(sodium aescinate, i.v. 2 mg/kg), 10 rats in each group. Another ten normal rats were selected as sham operation group. One times administration was administered on day 1 before establishing adhesion model, and administration for 3 d after modeling, once a day. On day 7 after operation, all of the rats were killed. The intestinal adhesion was graded and the adhesive tissues were taken for hydroxyproline determination. The levels of tumor necrosis factor(TNF)-α, interleukin(IL)-1β, and IL-6 in the serum were detected by ELISA.
ResultsCompared with the model group, sodium aescinate could obviously improve the severity of postoperative adhesion, markedly decrease hydroxyproline content in the adhesive tissues(P < 0.01), and significantly inhibit the levels of TNF-α, IL-1β, and IL-6 in the serum(P < 0.01).
ConclusionSodium aescinate could effectively prevent the formation of postoperative intestinal adhesion by inhibiting the expressions of inflammatory cytokines and decreasing the inflammatory response.
