ObjectiveThis study aimed to identify long non-coding RNAs (lncRNAs) that could serve as prognostic biomarkers for non-small cell lung cancer (NSCLC).MethodsThe Encyclopedia of RNA Interactomes (ENCORI) database was used to analyze the expression profile and RNA interaction network of lncRNA SNHG4 in NSCLC. Cell experiments were performed to determine the effects of lncRNA SNHG4 on the proliferation and migration of lung cancer cell line A549. The dual-luciferase reporter assay was employed to validate the interaction between SNHG4 and its potential targets.ResultslncRNA SNHG4 was highly expressed in NSCLC tissues. Kaplan-Meier survival analysis showed that patient survival rate was significantly correlated with SNHG4 expression levels. Knockdown of SNHG4 inhibited the proliferation, migration, and invasion of NSCLC cells while promoting cell apoptosis. Mechanistic studies identified miR-409-3p and DDX5 as direct targets of SNHG4. SNHG4 downregulated miR-409-3p expression in NSCLC cells, thereby relieving the inhibitory effect of miR-409-3p on DDX5 and promoting NSCLC cell growth. ConclusionlncRNA SNHG4 may exert its tumor-promoting role in NSCLC through the lncRNA SNHG4/miR-409-3p/DDX5 signaling pathway.
Objective To establish the reference intervals for platelet-related parameters among the elderly population in Longquanyi District, Chengdu. Methods From November 1st to December 31st 2021, elderly individuals aged between 60 and 80 years were selected from the Longquanyi branch of a natural population cohort study initiated by West China Hospital of Sichuan University in 2021. Complete blood cell analysis was performed to obtain the platelet-related parameters. IBM SPSS Statistics 25 software was used to analyze the data and establish the reference intervals for platelet-related parameters among the elderly population in Longquanyi District. Results A total of 1938 reference individuals were selected by inclusion and exclusion criteria, and finally 1669 reference individuals, including 665 males and 1004 females, were included to establish the reference intervals after excluding outliers. The reference range of blood platelet count was (95.03-301.32)×109/L for elderly men and (112.13-320.75)×109/L for elderly women, respectively. The reference range of mean platelet volume was 8.97-13.80 fL for elderly men and 9.10-13.90 fL for elderly women, respectively. The reference range of plateletcrit was 0.13%-0.30% for elderly men and 0.14%-0.32% for elderly women, respectively. The reference range of platelet distribution width was 15.80-16.90 for elderly men and 15.70-16.90 for elderly women, respectively. Conclusion It is of practical significance to establish platelet-related parameters in the elderly in local region.
