Objective
To review the latest progress in the major biological properties of adipose-derived stem cells (ADSCs) and ADSCs assisted autologous lipotransfer in breast repair and reconstruction.
Methods
Recent literature about ADSCs assisted autologous lipotransfer in breast repair and reconstruction was reviewed.
Results
ADSCs have multipotential differentiation capacity, and they could promote angiogenesis and regulate immune reactions. ADSCs assisted autologous lipotransfer can obtain satisfactory effectiveness in breast repair and reconstruction with few complications, but more studies are needed to confirm the long-term safety.
Conclusion
ADSCs assisted autologous lipotransfer has good effectiveness in breast repaired and reconstruction. But further clinical trials are needed to confirm the long-term safety.
【摘要】 目的 探討并分析導致肺曲霉病患者誤診的原因，為早期診斷并及時正確治療提供科學的依據。 方法 回顧性分析2010年1-4月間確診為肺曲霉病的3例患者在診治過程中被誤診的原因。 結果 3例患者均缺乏明顯的特異性臨床表現和影像學表現，最后確診均依據病理學活檢證實。 結論 肺部的曲霉菌感染缺乏特異性的臨床和影像學表現，及早行纖維支氣管鏡檢查或肺組織活檢可提高早期診斷率。【Abstract】 Objective To analyze the misdiagnostic causes of pulmonary aspergillosis. Methods The clinical data of three patients with pulmonary aspergillosis from January to April 2010 were retrospectively analyzed, and the misdiagnostic causes were analyzed. Result No specific clinical and imaging findings were found in the three patients, and pulmonary aspergillosis was finally diagnosed according to the pathological biopsy. Conclusion Pulmonary aspergillus lacks specific clinical and imaging manifestations; early fiberoptic bronchoscopy or pulmonary biopsy may improve the rate of accurate diagnosis.
【摘要】 目的 比較靶控誘導后Shikani喉鏡、Macintosh直接喉鏡和GlideScope?視頻喉鏡插管時的應激反應。 方法 選取2008年12月-2009年2月期間ASA Ⅰ~Ⅱ級、擬于全身麻醉下行擇期顱內占位病變切除術的患者30例，隨機分為Shikani喉鏡組（S組）、Macintosh直接喉鏡組（M組）與GlideScope?視頻喉鏡組（G組）。靶控異丙酚和瑞芬太尼誘導，分別采用上述3種喉鏡行經口氣管插管。記錄患者的心率、血壓，計算心率收縮壓乘積（RPP）。 結果 3組插管時間、心率、血壓和RPP比較差異無統計學意義（Pgt;0.05）。S組和M組插管后心率、血壓及RPP均較插管前顯著升高（Plt;0.05），而G組插管后的心率、60~300 s時的收縮壓、平均動脈壓和RPP與插管前比較，差異無統計學意義（Pgt;0.05）。 結論 3種喉鏡進行經口氣管插管時具有相似的血流動力學反應，GlideScope?視頻喉鏡更有利于循環穩定。【Abstract】 Objective To compare the hemodynamic responses of orotracheal intubations with GlideScope? videolaryngoscope, Macintosh direct laryngoscope, and Shikani optical stylet after target-controlled-infusion (TCI). Methods Thirty patients with American Society of Anesthesiologists (ASA) physical status Ⅰ-Ⅱ, scheduled for elective intracranial mass lesion surgery under general anesthesia were randomly allocated equally to Shikani optical stylet group (group S) Macintosh laryngoscope group (group M), and GlideScope? videolaryngoscope group (group G). After the patients became unconscious by TCI induction of propofpol and remifentanil, the endotracheal intubation were carried out through above three laryngoscope. The heart rate (HR), blood pressure and rate pressure product (RPP) were recorded. Results The differences of intubation time, HR, blood pressure and RPP in three groups were not statistically significant (Pgt;0.05). After intubation, the HR, blood pressure and RPP of group S and M were obviously higher than those before intubation (Plt;0.05); while there was no obvious change on the HR, systolic pressure at 60-300 s, mean arterial pressure and RPP of group G compared before intubation (Pgt;0.05). Conclusions There are similar hemodynamic responses in the three laryngoscope. GlideScope? is more advantageous to cycle stability.
Objective
To investigate the effect of preoperative rehabilitation on early function of joints after total hip arthroplasty by comparing with non-preoperative rehabilitation.
Methods
Between March 2010 and November 2011, 98 patients with hip disease were recruited in study. Of 98 patients, 49 received preoperative rehabilitation for 2 weeks in trial group, and 49 did not receive preoperative rehabilitation in control group. There was no significant difference in gender, age, disease duration, disease cause, preoperative visual analogue scale (VAS) score, and preoperative Harris score between 2 groups (P gt; 0.05).
Results
There was no significant difference in VAS score at each time point after operation between 2 groups (P gt; 0.05), but significant differences were found in Harris score (P lt; 0.05). At 3 days after operation, compliance evaluation results showed that 36 cases were of full compliance, and 13 cases were of partial compliance in trial group; 27 cases were of full compliance, 14 cases were of partial compliance, and 8 cases were of non-compliance in control group; there was significant difference between 2 groups (Z=2.286, P=0.002). The ambulation time was within 7 days in trial group, and was within 7 days in 47 cases and more than 7 days in 2 cases in control group; there was significant difference between 2 groups (Z=3.840, P=0.000).
Conclusion
Preoperative rehabilitation plays an important role in compliance, decreased bed rest time, and hip function improvement in patients after total hip arthroplasty.
Objective
To explore the effect of rolling compression loading bioreactor on chondrogenesis of rabbit bone marrow mesenchymal stem cells (BMSCs) with different loading parameters.
Methods
BMSCs were isolated from New Zealand rabbits, aged 2.5 months. BMSCs at passage 3 were used to prepare BMSCs-agarose gels (4 mm in diameter and height, respectively). Samples were divided into 8 groups: 10% (group A1), 20% (group A2), and 30% (group A3) compression groups (0.4 Hz, 3 h/ d) and 20 minutes (group B1), 3 hours (group B2), and 12 hours (group B3) rolling time groups and static culture (control groups). The living cell rate, the collagen type II and Aggrecan gene expressions, and glycosaminoglycan (GAG) content were determined, and histological staining was done at 24 hours, 7 days, 14 days, and 21 days after culture.
Results
At 14 and 21 days, the living cell rates of groups A1 and A2 were significantly higher than that of group A3 (P lt; 0.05), groups B1 and B2 were significantly higher than group B3 (P lt; 0.05). Collagen type II and Aggrecan gene expressions of the experimental groups at each time point were significantly higher than those of the control groups (P lt; 0.05); at 14 and 21 days, collagen type II and Aggrecan gene expressions of groups A1 and A2 were significantly higher than those of group A3, and groups B1 and B2 were also significantly higher than group B3 (P lt; 0.05). At 14 and 21 days, the GAG contents of groups A1 and A2 were significantly higher than those of group A3 (P lt; 0.05); groups B1 and B2 were also significantly higher than group B3 (P lt; 0.05). At 21 days, toluidine blue staining showed that obvious blue-staining and even cartilage lacunae were seen in groups A2 and B2, but light and quite rare blue-staining in groups A1, A3, B1, and B3.
Conclusion
The rolling compression loading bioreactor has great promotion effect on chondrogenesis of rabbit BMSCs with rolling parameters of 0.4 Hz, 3 hours, and 20% compression.
