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Mitochondria Mediates Induction of NOX1 Gene Expression by AngⅡ

摘要:目的: 探討在血管緊張素Ⅱ(AngⅡ)誘導血管平滑肌細胞(VSMCs)NOX1基因表達增加中線粒體所起的作用。 方法 :體外培養大鼠主動脈VSMCs,用線粒體呼吸鏈的抑制劑阻斷線粒體的作用,用熒光實時定量PCR檢測NOX1基因表達的量。 結果 :AngⅡ能夠誘導 NOX1基因的表達增加,線粒體呼吸鏈的抑制劑能夠抑制上述這一作用。 結論 :在大鼠的VSMCs中,AngⅡ誘導NOX1的增加通過線粒體呼吸的作用。Abstract: Objective: To detect the role of mitochondria involved in Angiotensin Ⅱ(Ang Ⅱ) induced NOX1 gene expression. Methods :Rat aortic vascellum smooth muscle cells(VSMCs) were cultured in vitro,and were treated with or without some inhibitors of complexs in mitochondrial respiratory chain. Realtime RTPCR was used to calculate the expression of NOX1 mRNA. Results :AngⅡ stimulated NOX1 gene expression,while some inhibitors of complexs in mitochondrial respiratory chain attenuated this progress.〖WTHZ〗Conclusion : Mitochondrial respiratory chain mediates expression of NOX1 gene in VSMCs by AngⅡ.