Purpose
To investigate the characteristics of intraocular growth of mice embryonic stem cells (ESC) in nude mice.
Methods
The undifferentiated murine ESC in vitro were transplanted into the eyes of nude mice.Mophological and immunohistochemical examinations were implemented.
Results
Two to three days after transplantation,yellowish-white granules and masses were seen inside the anterior chamber and vitreous cavity and enlarged gradually.Morphological examination showed that there were undifferentiated cells and differentiated cells in anterior chamber and vitreous cavity.The morphology and alignment of some differentiated cells were similar to those of the retina of nude mice.The cells were highly positive in NSE staining.
Conclusion
The transplanted ESC could grow in the eyes of nude mice and differentiate into neurons and retina-like structure.
(Chin J Ocul Fundus Dis，2000，16：213-284)
