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The Effects of Newcastle Disease Virus on Tumor Infiltrating Lymphocyte

ObjectiveTo study the effects of newcastle disease virus (NDV) on tumor infiltrating lymphocyte (TIL). MethodsTILs were dissociated from 6 cases of primary hepatic carcinoma. Each case was divided into 3 portions equally, and was added 20 μl NDV with HA titer of 1∶160 (NDV group), rIL2 with the end concentration of 1 000 u/ml (rIL2 group) and 0.9% sodium chloride (control group) respectively, then cultured in the condition of 37℃，5% CO2. CD3, CD4 and CD8 were assayed by flow cytometry. The contents of IL2, IFNγ and TNFβ in supernate of NDV group and control group were examined by ELISA.ResultsCoupled material revealed that CD3 and CD4 were significantly higher in NDV group than in the other two groups (P＜0.05, P＜0.01). CD8 had no significant changes in three groups. The contents of IL2, IFNγ and TNFβ in NDV group were significantly higher than those in control group (P＜0.01, P＜0.01 and P＜0.05 respectively). ConclusionNDV can activate TIL directly.