【摘要】 目的 研究腫瘤壞死因子相關凋亡誘導配體（TRAIL）蛋白對SKOV3移植瘤細胞半胱天冬氨酸蛋白酶-3（Caspase-3）表達的影響及其與腫瘤細胞凋亡的關系。 方法 建立雌性裸小鼠SKOV3移植瘤24只，隨機分為4組，每組6只。TRAIL組單用重組人TRAIL蛋白（10 μg/kg），順鉑（DDP）組單用DDP（3 mg/kg），TRAIL+DDP組聯合使用TRAIL蛋白（10 μg/kg）和DDP（3 mg/kg），空白對照組給予0.5 mL生理鹽水。經處理后，各組的組織切片用免疫組織化學染色檢測Caspase-3的表達和末端脫氧核苷酸轉移酶介導核苷酸缺口標記技術（TUNEL）檢測腫瘤細胞凋亡指數。 結果 Caspase-3的表達水平在TRAIL組（171.67±14.38）、DDP組（172.50±14.75）、聯合組（230.00±40.99）中均明顯高于對照組（135.83±16.25）（Plt;0.05）。SKOV3移植瘤細胞凋亡指數在空白對照組、TRAIL組、DDP組和聯合組分別為16.67±5.43、33.17±8.42、24.33±4.59和40.50±6.16，TRAIL組和聯合組細胞凋亡指數較空白對照組和DDP組明顯增高（Plt;0.05）。 結論 TRAIL蛋白使卵巢癌移植瘤細胞的Caspase-3表達增強，TRAIL蛋白促進腫瘤細胞凋亡發生。【Abstract】 Objective To investigate the effects of Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on the expression of Cysteine/aspartic acid specific protease- 3 (Caspase-3) in SKOV3 ovarian tumor cells and its relationship with the apoptosis of the ovarian tumor xenografts in nude mice. Methods Twenty-four nude mice with SKOV3 cell line ovarian tumor were randomly divided into four groups with 6 in each group. TRAIL (10 μg/kg) was given to the mice in the TRAIL group; DDP (3 μg/kg) was given to the mice in the DDP group; TRAIL (10 μg/kg) and DDP (3 μg/kg) were given to the mice in the TRAIL+DDP group; and 0.5 mL of saline solution was give to the mice in the control group. The expression of Caspase-3 was detected with immunohistochemistry. The apoptosis index (AI) of cells was determined by Terminal deoxynucleotidyl transferase mediated-dUTP nick end labeling (TUNEL). Results The expression of Caspase-3 in the TRAIL group (171.67±14.38), DDP group (172.50±14.75), and TRAIL+DDP group (230.00±40.99) was significantly higher than that in the control group (135.83±16.25) (Plt;0.05). The apoptosis index for the control group, TRAIL group, DDP group and TRAIL+DDP group was 16.67±5.43, 33.17±8.42, 24.33±4.59, and 40.50±6.16, respectively. The apoptosis index for the TRAIL group and the TRAIL+DDP group was significantly higher than that in the control group and the DDP group (Plt;0.05). Conclusion Soluble TRAIL has an effect on enhancing the expression of Caspase-3 in implanted tumor in nude mice. TRAIL protein can inhibit the growth of SKOV3 cells in nude mice by inducing cell apoptosis.
Objective To compare and evaluate the sensitivity, specificity, accuracy, negative and positive predictive values, negative and positive likelihood ratios of colposcopically directed biopsy and diagnostic cone biopsy in patients with cervical intraepithelial neoplasia. Methods We searched PubMed, CBMdisc, CMCC, CNKI, and VIP to March 2004, and Cochrane Library (Issue 4, 2003). Related journals published from 1970 to 2003 and unpublished papers were hansearched. Diagnostic studies which employed colposcopically directed biopsy or diagnostic cone biopsy and compared with golden standard (pathological diagnosis of specimens obtained through therapeutic conization or hysterectomy) were included and meta-analysis was performed. Participants were clinically suspected of pre-cancerous cervical lesions. Quality of studies was assessed, and SROC curve by Diagnostic and Screening Group of the Cochrane Collaboration was used to perform meta-analysis. Parameters were sensitivity, specificity, accuracy, predictive values, and likelihood ratio. Results Twenty six studies (3 376 patients ranging from 2 to 604 patients/per study) met the inclusion criteria. The quality of studies was generally poor.Before sensitivity analysis, superiority of diagnostic cone biopsy (sensitivity and specificity: 0.83) was shown over colposcopically directed biopsy (sensitivity and specificity: 0.76) (P＜0.001); while after sensitivity analysis the results reversed (sensitivity of diagnostic cone biopsy was 0.58 and its specificity was 0.61; sensitivity and specificity of colposcopically directed biopsy increased to 0.84) (Plt;0.001). Conclusions No definite conclusioncan be drawn as to which method is superior. To make further analysis, more studies with high quality are needed.
