Objective To investigate the effects of adenosine 2A receptor (A2AR) activation on oxidative stress in small-forsize liver transplantation. Methods A rat orthotopic liver transplantation model was performed using 40% graft, 18 recipients were given intravenously saline (control group), CGS21680 (A2AR agonist, CGS21680 group) or ZM241385 (A2AR antagonist, CGS21680+ZM241385 group) randomly. Aspartate aminotransferase (AST), enzymatic antioxidants 〔superoxide dismutase (SOD); catalase (CAT); glutathione peroxidase (GSH-Px)〕, non-enzymatic antioxidants 〔ascorbic acid (AA); glutathione (GSH); α-tocopherol (TOC)〕 and lipid oxidant metabolites malondialdehyde (MDA) were measured and analyzed at 6 h after reperfusion. Results Compared with the control group and CGS21680+ZM241385 group, A2AR activation increased the activities of SOD and GSHPx (Plt;0.05), reduced the productions of AST and MDA (Plt;0.05), increased the levels of AA, GSH and TOC (Plt;0.05) in CGS21680 group. But there was no significant change in CAT activity (Pgt;0.05) among 3 groups. Conclusions A2AR activation improves the antioxidant enzyme activities, promotes the production of antioxidants, and slowes down the increase in MDA level, depresses of the increase in AST activity. A2AR activation suppresses oxidative damage and increases the antioxidant capacity which in turn minimizes their harmful effects of ischemia-reperfusion in small-for-size liver transplantation.
Objective To study the effects of adenosine 2A receptor activation on activation, proliferation, and toxicity of T lymphocytes stimulated by phytohemagglutinin (PHA) in vitro. Methods A model of activated T cells was established by stimulating the cells with PHA. Those T cells were treated with different concentrations of adenosine 2A receptors agonist (0.01 μmol/L, 0.1 μmol/L, 1 μmol/L, and 10 μmol/L CGS21680). The expressions of CD69, CD25 and proliferation of T cells were measured by fluorescent antibody stain and flow cytometry. ELISA method was used to detect IL-2 and INF-γ levels. Results All concentrations of CGS21680 significantly inhibited the expressions of CD25 and CD69 on PHA-stimulated T cells surface and proliferation of T cells （Plt;0.05， Plt;0.01）. IL-2 and INF-γ secreted by T cells were significantly suppressed, too （Plt;0.01）. Conclusion Activation of adenosine 2A receptor can effectively inhibit the activation, proliferation, and toxicity of T cells in vitro.
ObjectiveTo evaluate the short-term safety and efficacy of domestic porcine small intestinal submucosal decellularized matrix (SIS) mesh in laparoscopic inguinal hernia repair (LIHR), and observe its clinical outcomes related to tissue repair. MethodsA multicenter open-label single-arm study included 169 patients undergoing LIHR with domestic SIS mesh. The primary assessment index was the excellent rate on postoperative Day 180, the secondary efficacy indexes were the hernia recurrence rate, incision healing rate, wound healing time, mesh infection, chronic pain, and discomfort at the repair site after the operation. ResultsAll surgeries were successfully completed, with the excellent rate was 100% on postoperative Day 180 which was significantly higher than the value of preset target (91%). The incision healing rate was 100%, and the median wound healing time was 8 days. 97.04% patients showed immediate painless after surgery, 98.20% patients were painless on postoperative Day 7, all patients were painless on postoperative Day 28. Immediately after the operation, 97.04% patients had no discomfort at the repair site, 98.20% patients had no discomfort on postoperative Day 7, all patients had no discomfort on postoperative Day 28. There was no recurrence or infection after operation. ConclusionDomestic SIS mesh is safe and effective in the laparoscopic treatment of inguinal hernia, and is worth popularizing for clinical use.
