【摘要】目的探討丹酚酸(Sal)B 配伍丹皮酚對大鼠心肌缺血再灌注損傷(MIRI)的保護作用。方法大鼠心肌缺血60 min 后再灌注90 min 造成大鼠心肌缺血再灌注損傷模型,測定給藥后心肌組織勻漿、血漿中內皮素(ET)、血清中一氧化氮(NO)和一氧化氮合酶(NOS)的變化,并通過病理組織學檢查,觀察Sal B 配伍丹皮酚5、10、15 mg/kg對大鼠MIRI不同濃度和不同給藥方法的治療作用。結果Sa1 B配伍丹皮酚,中、高劑量組均能減少心肌組織和血漿中ET 的含量,提高NOS 的活性,增加NO的釋放(Plt;005)。結論Sal B減輕大鼠MIRI 可能是通過調節ET/NO 系統的平衡,維持冠脈血管張力,改善心肌能量代謝障礙實現的。
ObjectiveTo analyze the expression and clinical significance of cyclin-dependent kinase 1 (CDK1) in lung adenocarcinoma by bioinformatics.MethodsBased on the gene expression data of lung adenocarcinoma patients in The Cancer Genome Atlas (TCGA), the differential expression of CDK1 in lung adenocarcinoma tissues and normal lung tissues was analyzed. The expression of CDK1 gene in lung adenocarcinoma was analyzed by UALCAN at different angles. Survival analysis of different levels of CDK1 gene expression in lung adenocarcinoma was performed using Kaplan-Meier Plotter. Correlation Cox analysis of CDK1 expression and overall survival was based on clinical data of lung adenocarcinoma in TCGA. Gene set enrichment analysis was performed on gene sequences related to CDK1 expression in clinical cases. The protein interaction network of CDK1 from Homo sapiens was obtained by STRING. CDK1-related gene proteins were obtained and analyzed by the web server Gene Expression Profiling Interactive Analysis (GEPIA).ResultsBased on the analysis of TCGA gene expression data, CDK1 expression in lung adenocarcinoma was higher than that in normal lung tissues. UALCAN analysis showed that high CDK1 expression may be associated with smoking. Survival analysis indicated that when CDK1 gene was highly expressed, patients with lung adenocarcinoma had a poor prognosis. Univariate and multivariate Cox regression analysis of CDK1 expression and overall survival showed that high CDK1 expression was an independent risk factor for survival of patients with lung adenocarcinoma. Gene set enrichment analysis revealed that high CDK1 expression was closely related to DNA replication, cell cycle, cancer pathway and p53 signaling pathway.ConclusionCDK1 may be a potential molecular marker for prognosis of lung adenocarcinoma. In addition, CDK1 regulation may play an important role in DNA replication, cell cycle, cancer pathway and p53 signaling pathway in lung adenocarcinoma.
