ObjectiveTo investigate the effects of short-time hyperoxia ventilation on lung tissue and pulmonary surfactant proteins C and D (SP-C and SP-D) in rats.MethodsSixteen male Sprague-Dawley rats were randomly divided into two groups (n=8): hyperoxia group (FiO2=0.90), air group (FiO2=0.21). Tracheal intubations were administrated after anesthesia, and rats in two groups were exposed hyperoxia or air ventilation for 4 h. At the same time, carotid artery blood gas was analyzed after 2 h and 4 h of ventilation, then oxygenation index (OI) was calculated. Four hours later, the anterior lobe of right lung was taken to observe the pathological change and the injury level was scored. The middle lobe of right lung was prepared for making tissue homogenate, and the remaining part of the lung was used to measure the wet/dry weight (W/D) ratio. The bronchoalveolar lavage fluid (BALF) was prepared in left lung. The content of SP-C and SP-D were detected in lung tissue homogenate and BALF by ELISA.ResultsComparing with hyperoxia group, the arterial partial pressure of oxygen, lung histopathology score and lung W/D ratio in air group were significantly increased (P<0.05), but OI, the content of SP-C and SP-D in lung tissue homogenate and BALF were significantly decreased (P<0.05).ConclusionHyperoxia ventilation for 4 h in rats can cause lung injury histologically, and reduce the concentration of SP-C and SP-D apparently in the lungs.
Objective To study the predictive value of inspiratory capacity (IC) and total lung capacity (TLC) on acute exacerbation of patients with chronic obstructive pulmonary disease (COPD). Methods The in-patients due to acute exacerbation of COPD (AECOPD) from February 2017 to March 2019 were recruited in the study. Research data were collected during the recovery period and one year follow-up , and the patients were divided into two groups according to whether there was another acute exacerbation. Results A total of 372 patients were included in the study. In the patients with acute exacerbation, the age and TLC as a percentage of the expected value (TLC%pred) were higher, while IC as a percentage of the expected value (IC%pred) and the ratio of IC and TLC (IC/TLC) were lower. Univariate analysis revealed that IC/TLC≤25% was a significant predictor of acute exacerbation (P<0.001) after the AECOPD patients were discharged. Multivariable analysis revealed age (OR=1.25, 95%CI 1.17 to 1.44, P<0.001) and IC/TLC≤25% (OR=1.68, 95%CI 1.36 to 2.07, P<0.001) were independent risk factors of AECOPD.Conclusions Decreased IC/TLC significantly correlates with poor prognosis of COPD. IC/TLC≤25% is an independent risk factor for acute exacerbation.
【摘要】 目的 探討喉癌組織中 EGFL7基因 mRNA表達與喉癌分化、轉移及臨床預后的關系。 方法 收集2008年5—11月共42例行喉癌手術患者的切除標本。用免疫組織化學法檢測EGFL7蛋白在42例喉癌組織和配對癌旁組織中的表達情況，提取腫瘤及癌旁組織配對標本的總 RNA，用逆轉錄（RT）-PCR方法測定EGFL7基因表達，蛋白質印跡法測定EGFL7蛋白的表達，并結合臨床資料，對 EGFL7基因差異表達與喉癌患者臨床表現的相關性進行分析研究。 結果 對42例配對標本分別進行 EGFL7 mRNA熒光檢測比較，30例標本的腫瘤組織中EGFL7基因mRNA表達明顯高于癌旁正常喉組織。22例標本的腫瘤組織中EGFL7蛋白表達明顯高于癌旁正常喉組織。EGFL7 mRNA的表達與喉癌淋巴結轉移和浸潤深度密切相關（Plt;0.05），而與患者的性別、年齡、吸煙、腫瘤分化程度等無關（Pgt;0.05）。 結論 EGFL7基因在喉癌組織中表達狀態與喉癌的生長和浸潤轉移關系密切，EGFL7基因可望作為喉癌病情發展及指導臨床治療的標記物之一。【Abstract】 Objective To investigate the association of EGF-like-domain, multiple 7 (EGFL7) mRNA expression level with the differentiation, metastasis and prognosis of laryngocarcinoma. Methods Tissue specimens were obtained from 42 patients undergoing surgery for laryngocarcinoma between May and November, 2008. Immunohistochemistry was used to detect the level of EGFL7 in the 42 tumor tissues and matched adjacent normal tissues. Reverse transcriptional PCR (RT-PCR) was performed for amplification of EGFL7 mRNA from the 42 tumor tissues and matched adjacent normal tissues, and westerblot was adopted to determine EGFL7 protein expression. The differential EGFL7 mRNA expression was analyzed for its association with the clinical manifestations of the patients. Results EGFL7 mRNA expression was detected in all the laryngocarcinoma tissues and normal tissues adjacent to the carcinoma using fluorescence method. EGFL7 mRNA expression was significantly higher in the tumor tissues than in the adjacent tissues in 30 cases, and EGFL7 protein expression was also significantly higher in the tumor tissues than in the adjacent normal laryngeal tissues in 22 cases. Expression of EGFL7 mRNA was highly correlated with lymph node metastasis and T classification (Plt;0.05), but was not correlated with the patients’ gender, age, or tumor differentiation (Pgt;0.05). Conclusions EGFL7 mRNA expression is correlated closely with the differentiation and metastasis of laryngocarcinoma. EGFL7 may serve as a marker for assessing the progression of laryngocarcinoma and provide assistance for clinical therapeutic decisions.
ObjectiveTo systematically review the detection rate of sub-health status of Chinese college students. MethodsThe CNKI, WanFang Data, CBM, VIP, PubMed, EMbase and Web of Science databases were searched to collect cross-sectional studies on the detection rate of sub-health among Chinese college students from inception to February 1, 2023. Two researchers independently screened the literature, extracted data and evaluated the risk of bias of the included studies. Meta-analysis was performed using Stata 17.0 software. At the same time, GIS technology was used to analyze the spatial distribution of the detection rate of sub-health status of college students in China. ResultsA total of 44 articles were included, with a total sample size of 63 435 cases, including 29 255 cases of sub-health status. The results of meta-analysis showed that the detection rate of sub-health status of Chinese college students was 51.2% (95%CI 44.1 to 58.4). The temporal distribution showed a gradual decrease in the detection rate of sub-health states among college students between 2016 and 2020, with a rebound after 2020. The detection rates of sub-health states among college students in different regions and survey instruments varied significantly, with 74.1%, 61.8%, 58.4%, 56.1%, 47.0%, 42.3% and 21.6% in Southwest, Northeast, South, North, Central, East and Northwest China, respectively. The detection rates of sub-health states among college students in Jilin, Sichuan and Hebei provinces were the top 3, and Shanxi province had the lowest detection rate. ConclusionThe detection rate of university sub-health status in China is high, and the detection rate decreased significantly from 2016 to 2020, with a rebound trend in recent years, and there are differences in the detection rate of university sub-health status in different regions and survey instruments. Due to the limitation of the quality and quantity of the included studies, the above findings need to be verified by more high-quality literature.
Objective To explore the safety and feasibility of ultra-fast-track anesthesia (UFTA) in transcatheter aortic valve replacement (TAVR) under the concept of enhanced recovery after surgery (ERAS). Methods A retrospective analysis was conducted on the clinical data of patients who underwent TAVR at the First Hospital of Lanzhou University between January 2022 and June 2024. Based on the anesthesia technique, patients were categorized into an ultra-fast-track anesthesia group (group U) and a conventional general anesthesia group (group C). The general patient characteristics, operative duration, length of hospital stay, hospitalization costs, and complications were compared between the two groups. Results A total of 101 patients were ultimately included in the study, comprising 60 males and 41 females, with a mean age of (67.85±7.73) years. Among them, 57 patients were assigned to the group U and 44 to group C. Compared with the group C, the group U showed significantly shorter postoperative extubation time (0.18 h vs. 4.88 h), CCU stay (22 h vs. 37 h), hospital stay (8 d vs.13 d), and lower hospitalization costs (234 300 yuan vs. 251 100 yuan) (P<0.05). In addition, the incidences of pulmonary infection (3.5% vs. 15.9%) and atelectasis (3.5% vs. 15.9%) within 30 days after surgery in the group U were significantly reduced (P<0.05).There was no statistically significant difference in the intraoperative mean arterial pressure, heart rate, or other complications such as postoperative arrhythmia, pervalvular leakage, and cerebral infarction between the two groups (P>0.05). Conclusion UFTA not only ensures the safety of TAVR patients, but also significantly enhances postoperative recovery, shortens recovery time, and optimizes medical resource utilization,.
Objective To survey the prevalence of coexisting chronic obstructive pulmonary disease ( COPD) and chronic heart failure in Chengdu communities. Methods A cross-sectional study was performed in the population aged over 40 years in eight communities of Chengdu city. The subjects were selected by stratified cluster random sampling. Using the same protocol and questionnaire, all participants underwent medical history taking, physical examination and spirometry. Results Of 354 participating patients with a diagnosis of COPD, 74 ( 20. 90% ) cases were complicated with chronic heart failure. The prevalence of chronic heart failure in COPD in male was significantly higher than that in female ( 22. 69%vs. 18. 12% , P lt; 0. 05) . The major causes of chronic heart failure were hypertension ( 31. 64% ) , ischaemic heart disease ( 18. 93% ) , chronic pulonary heart disease ( 17. 51% ) and diabetes ( 11. 86% ) .Conclusions The prevalence of coexisting COPD and chronic heart failure in Chengdu city is significantly higher than the average level of the whole country, which warrant more attention in prevention and treatment of COPD in communities.
【Abstract】 Objective To survey the prevalence and risk factors of chronic obstructive pulmonary disease ( COPD) , and establish database for community intervention of COPD.Methods A cluster-randomsampling survey was carried out. The population aged over 40 years old in eight communities of Chengdu were investigated from August 2007 to August 2009 using the standard protocol and questionnaire. The spirometry was performed for each participant. Results A total of 4315 residents were enrolled in this survey and 3687 case reports were analyzed, including 1529 males and 2148 females, with an average age of 60. 35 ±13. 21 years old. Total prevalence of COPD was 9. 6% . The prevalence of COPD in the males was significantly higher than that in the females ( 14. 0% vs. 6. 4%, P lt;0. 01) . The prevalence of COPD increased with the age. The major risk factors were cigarettes smoking ( OR = 3. 012, 95% CI 2. 390-3. 797, P lt; 0. 01) and indoor cooking smoking ( OR = 2. 546, 95% CI 1. 734-3. 425, P lt; 0. 01) . Conclusion The prevalence of COPD in Chengdu city is significantly higher than average level of the whole country, which warrant moreattention to better prevention and treatment of COPD in communities.
