Objective To investigate the physicochemical properties of pure titanium surface grafted with chlorhexidine (CHX) by phenolamine coating, and to evaluate its antibacterial activity and osteoblast-compatibility in vitro. MethodsControl group was obtained by alkali and thermal treatment, and then immersed in the mixture of epigallocatechin-3-gallate/hexamethylene diamine (coating group). Phenolamine coating was deposited on the surface, and then it was immersed in CHX solution to obtain the grafted surface of CHX (grafting group). The surface morphology was observed by scanning electron microscope, the surface element composition was analyzed by X-ray photoelectron spectroscopy, and the surface hydrophilicity was measured by water contact angle test. Live/dead bacterial staining, nephelometery, and inhibition zone method were executed to evaluate the antibacterial property. Cytotoxicity was evaluated by MTT assay and cell fluorescence staining. Bacteria-MC3T3-E1 cells co‐culture was conducted to evaluate the cell viability on the samples under the circumstance with bacteria. Results Scanning electron microscope observation results showed that deposits of coating group and grafting group increased successively and gradually covered the porous structure. X-ray photoelectron spectroscopy results showed the peak of N1s enhanced and the peak of Cl2p appeared in grafting group. Water contact angle test results showed that the hydrophilic angle of three groups increased in turn, and there was significant difference between groups (P<0.05). Live/dead bacteria staining results showed that the grafting group had the least amount of bacteria adhered to the surface and the proportion of dead bacteria was high. The grafting group had a transparent inhibition zone around it and the absorbance (A) value did not increase, showing significant difference when compared with control group and coating group (P<0.05). MTT assay and cell fluorescence staining results showed that the number of adherent cells on the surface of the grafting group was the least, but the adherent cells had good proliferation activity. Bacteria-cell co-culture results showed that there was no bacteria on the surface of grafting group but live cells adhered well. ConclusionCHX-grafted phenolamine coating has the ability to inhibit bacterial adhesion and proliferation, and effectively protect cell adhesion and proliferation in a bacterial environment.
ObjectiveTo evaluate the short-term safety and efficacy of domestic porcine small intestinal submucosal decellularized matrix (SIS) mesh in laparoscopic inguinal hernia repair (LIHR), and observe its clinical outcomes related to tissue repair. MethodsA multicenter open-label single-arm study included 169 patients undergoing LIHR with domestic SIS mesh. The primary assessment index was the excellent rate on postoperative Day 180, the secondary efficacy indexes were the hernia recurrence rate, incision healing rate, wound healing time, mesh infection, chronic pain, and discomfort at the repair site after the operation. ResultsAll surgeries were successfully completed, with the excellent rate was 100% on postoperative Day 180 which was significantly higher than the value of preset target (91%). The incision healing rate was 100%, and the median wound healing time was 8 days. 97.04% patients showed immediate painless after surgery, 98.20% patients were painless on postoperative Day 7, all patients were painless on postoperative Day 28. Immediately after the operation, 97.04% patients had no discomfort at the repair site, 98.20% patients had no discomfort on postoperative Day 7, all patients had no discomfort on postoperative Day 28. There was no recurrence or infection after operation. ConclusionDomestic SIS mesh is safe and effective in the laparoscopic treatment of inguinal hernia, and is worth popularizing for clinical use.
