【摘要】 目的 考察用烏頭堿水解物、新烏頭堿水解物、次烏頭堿水解物的含量作為附片水煎劑及附片質量控制指標的可行性。 方法 采用高效液相色譜分析法，色譜柱為Shimpack CLC-ODS，以甲醇-乙酸銨溶液（0.2 mol/L）（200︰210）為流動相，流速1.0 mL/min，檢測波長為241 nm，對烏頭堿新，烏頭堿、次烏頭堿水解物的含量測定進行研究。 結果 3種水解物檢測方法測得水解物在進樣范圍有良好線性關系，重現性好，穩定性好，生藥提取時間宜選定為40 min，不同批號的附片中3種水解物含量差別較大。 結論 此方法簡便，使用新烏頭堿、烏頭堿、次烏頭堿的水解物作為含附片制劑的質量控制指標是可行的。【Abstract】 Objective To examine the feasibility of regarding the hydrolyzate from aconitine, new aconitine, and hypaconitine to be the decoction of radix aconite and the quality control index. Methods High performance liquid chromatographic method (HPLC) method was adopted. Shimpack CLC-ODS column with UV detection at 241 nm was used. The mobile phase consisted of (200∶210), and the flow rate was 1.0 mL/min. Results The content of the hydrolysis objects in the water decoction and toxicity were correlated with each other, and the contents of hydrolysis were significantly different among different groups of radix aconiti. Conclusion This method is simple, accurate and effective for content research of hydrolyzate from aconitine-type alkaloids in Monkshoo decoction of Radix Aconiti, and it is feasible to take it as the quality control index.
