ObjectiveTo detect the expressions of signal peptide-CUB-EGF-like domain containing protein 3 (SCUBE3) and specificity protein 1 (SP1) in breast cancer tissues, and explore relations between their protein expressions and clinicopathologic features or prognosis.MethodsFrom February 2013 to October 2015, the breast cancer tissues and the corresponding adjacent normal breast tissues of 80 women patients with breast cancer in the Mianyang Central Hospital were selected, and the expressions of SCUBE3 and SP1 proteins in the tissues were detected by immunohistochemistry. The relations between the expressions of SCUBE3 and SP1 and clinicopathologic parameters of breast cancer were analyzed, the correlation between the SCUBE3 and SP1 was analyzed by Spearman rank correlation analysis. Kaplan-Meier method was used to analyze the survival of patients with breast cancer; and Cox proportional hazards regression model was used to analyze the risk factors of overall survival of patients with breast cancer.ResultsThe positive rates of SCUBE3 and SP1 proteins expressions in the breast cancer tissues were higher than those in the corresponding adjacent normal breast tissues (P<0.05). The positive rates of SCUBE3 and SP1 protein expressions were higher in the breast cancer tissues with lymph node metastasis and molecular subtypes of Luminal A or B (P<0.05), and the positive rates of SCUBE3 protein expression were higher in the breast cancer tissues with TNM stage Ⅱ–Ⅳ and high Ki67 (P<0.05). The retsult of Spearman rank correlation analysis showed that the positive rates of SCUBE3 and SP1 proteins expressions in the breast cancer tissues was positive correlation (χ2=7.979, rs=0.316, P=0.005). Kaplan-Meier curve showed that the overall survival of the patients with positive expression of SCUBE3 or SP1 protein was worse than that of the patients with negative expression (χ2=4.042, P=0.044; χ2=10.676, P=0.001). The Cox proportional hazards regression model multivariate analysis showed that the positive SCUBE3 (HR=6.020, P=0.016), positive SP1 (HR=4.077, P=0.018), lymph node metastasis (HR=3.518, P=0.017), and higher Ki67 expression (HR=7.989, P<0.001) were the independent risk factors of overall survival for the patients with breast cancer.ConclusionPositive rates of SCUBE3 and SP1 proteins expressions in breast cancer tissues are higher and there is a positive correlation between them, which are closely related to clinicopathologic parameters such as lymph node metastasis and molecular subtypes and prognosis of patients with breast cancer.
【摘要】 目的 比較喉罩和氣管內插管吸入七氟烷全麻用于小兒無痛苦纖支鏡檢查的麻醉效果、蘇醒時間、蘇醒質量。 方法 將2008年3月-2009年3月40例行纖支鏡檢查的患兒隨機分為喉罩組（L組，21例）和氣管內插管組（T組，19例）。兩組均采用逐漸誘導法吸入七氟烷，靜脈給予芬太尼1 μg/kg；L組置入喉罩，T組氣管插管后控制呼吸。手術中，兩組均吸入2%～5%七氟烷維持麻醉。分別記錄麻醉前（T0）、麻醉后纖支鏡進入前（T1）、進鏡至咽部（T2）、聲門部（T3）、氣管內（T4）及第15 min（T5）時的血壓（BP）、心率（HR）、MAP和動脈血氧飽和度（SPO2）。觀察纖支鏡檢查期間有無嗆咳、氣道痙攣或體動；記錄停藥至拔管的時間，蘇醒后是否再入睡及麻醉滿意度。 結果 兩組HR在T1、T3、T4時升高，與T0時比較，有統計學意義（Plt;0.05）；其中HR在T1時T組高于L組，組間比較有統計學意義（Plt;0.05）；MAP在T1、T2、T3、T4時，T組低于L組，組間比較有統計學意義（Plt;0.05）。兩組患兒鏡檢期間均無嗆咳、氣道痙攣或體動；L組蘇醒時間短于T組（Plt;0.05），蘇醒后再入睡率低于T組（Plt;0.05），麻醉滿意度高于T組（Plt;0.05）。 結論 喉罩吸入七氟烷全麻用于小兒無痛苦纖支鏡檢查，能保證穩定的血流動力學狀態，蘇醒快速，效果滿意。【Abstract】 Objective To assess the feasibility and safety of Laryngeal mask combined with sevoflurane in painless fiberoptic bronchoscopy anesthesia in children. Methods Forty children from March 2008 to March 2009 were randomized divided into laryngeal mask airway group (group L) and endotracheal intubation group (group T). Anaesthesia was induced and maintenanced with 2%-5% sevoflurane and fentanyl 1 μg/kg. The blood pressure (BR), heart rate (HR) and oxygen saturation by pulse oximeter (SPO2)were recorded before anaesthesia (T0), immediately after anaesthesia induction (T1), when FOB at the level of pharynx(T2), vocal cords (T3), trachea (T4) and in 15 min of the FOB (T5). Extubation time, recovery quality and anesthesia effects were also analyzed. Results Compared with T0, there were significant increases in HR at T1、T3 and T4 (Plt;0.05), and HR was significantly higher in the group T than that in the group L at T1 (Plt;0.05). MAP was significantly lower in group T than that in group L at T1、T2、T3 and T4, respectively(Plt;0.05). Recovery time was significantly shorter in group L than that in group T. Incidence of sleep after recovery was lower in group L than that in group T. Anesthesia satisfaction was higher in the group L than that in the group T. Conclusions Laryngeal mask combined with Sevoflurane provide satisfactory anesthesia for painless fiberoptic bronchoscopy in children.
【摘要】 目的 探討葡萄糖轉運蛋白Ⅰ型（glucose transporter 1，GLUT1）和腫瘤增殖細胞核抗原Ki-67在卵巢上皮性腫瘤組織中的表達及其臨床意義。 方法 收集2000年1月-2008年6月不同卵巢上皮性腫瘤病變患者119例的組織標本，采用免疫組織化學SP二步法檢測腫瘤組織中GLUT1和Ki-67的表達情況。 結果 卵巢交界性、惡性上皮性腫瘤灶性或廣泛高表達GLUT1和Ki-67，其表達強度有差異。卵巢良性上皮性腫瘤不表達GLUT1和Ki-67。在卵巢癌中GLUT1及Ki-67的表達強度與病理分級、臨床分期、預后有關。GLUT1表達強度與病理分型無關，Ki-67表達強度與病理分型有關。 結論 卵巢上皮性腫瘤組織中GLUT1和Ki-67的表達具有相關性，其表達強度與腫瘤的良惡性質和增殖狀態有關，二者同時檢測可以全面了解卵巢上皮性腫瘤的性質、卵巢癌惡性程度和生物學行為，對于判斷腫瘤的性質和預后有一定價值。【Abstract】 Objective To investigate the expression and clinical significance of glucose transporter-1 (GLUT1) and tumor proliferating karyon antigen Ki-67 in epithelial ovarian tumor tissue. Methods Immunohistochemistry SP method was used to detect the expression of GLUT1 and Ki-67 protein in epithelial ovarian tumor tissues from 119 patients diagnosed in our hospital from January 2000 to June 2008. Results The expressions of GLUT1 and Ki-67 had local or abroad higher expressions in the borderline and malignant epithelial ovarian tumor, and the expressive intensity was different. In benign tumors, the expression was negative. The expressive intensity of GLUT1 and Ki-67 had correlation with the grade, stage, and prognosis in malignant tumors. The expressive intensity of GLUT1 had no correlation with the type of malignant tumors, while Ki-67 related to the pathological types. Conclusion The expressions of GLUT1 and Ki-67 have relativity. The expressive intensity of GLUT1 and Ki-67 relates to the character and proliferation of epithelial ovarian tumors. The combined detection GLUT1 and Ki-67 is helpful to know the character of epithelial ovarian tumors, the malignant degree and biologic behavior of ovarian carcinoma, which is useful in estimating the character and prognosis of epithelial ovarian tumors.
