To study the prognostic significance of proliferative cell nuclear antigen(PCNA)in bile duct carcinoma,expression of PCNA protein was studied immunohistochemically in 30 patients with bile duct carcinoma.Results:86.7 percent of bile duct carcinomas showed PCNA positive staining and significiant difference was observed between malignant and benign tissues.These results suggest that proliferative activity of malignant tissues was ber than that of the benign ones.In patients with cancer of stages 3,the mean survial time for those with high proliferative activity was 13 months in constrast with 26 months for those with low activity.PCNA is of prognostic significance for bile duct carcinoma patients.
The contents of lipid peroxides(LPO)and vitamin E(V.E)and some functional index and histologic changes in the lungs from the the rabbit models of acute cholangitis of severe type(ACST)were measured dynamically.The results revealed that the V.E content decreased strikingly from 6 hours and the LPO level increased progressivelg from 12 hours in the lungs.Simultanuosly,the congestion and neutrophil infiltreation in the lung mesenchyme,and the endothelial cell damage and thrombosis in the lung blood capillaries had been observed.These suggest that acute lung injury induced by ACST is referable to the lipid peroxidation damage to the lung blood capillaries which is due to increased LPO and decreased antioxidants including V.E.
The authors observed the progressive changes of the platelet activity in 25 cases of acute cholangitis severe type(ASCT)within 2 weeks of pre-and-post-operation.The results revealed that there are signficant changes of the platelet activity in ASCT.The levels of the platelet activity were proportional to the severity of disease and degree of biliary duct obstruction.Persistency of abnormality of platelet activity may predict the worse of disease and bad prognosis.The results are useful to understand the complex changes of pathophysiology in ACST,to the mechanism of multiple organic failure.
