ObjectiveTo investigate the disinfection effect of dry-fogging hydrogen peroxide (DFHP) on ambulance inner surfaces.MethodsThis study was carried out using simulated field test and field test from October to December 2018. In the simulated field test, the carriers with Geobacillus stearothemopilus (ATCC12980) spores were placed in 6 places in the ambulance, and disinfected for 60 minutes with DFHP of 0.38–0.72 g/m3. The carriers were cultured for up to 7 days to observe whether the bacteria were eliminated. Before and after the DFHP disinfection, the microbial sampling of the surface in the ambulance was carried out, and the colonies were counted after the cultivation.ResultsThe eliminating rate of the bacteria carriers on the uncovered surface was 100% (20/20), and that of the covered surface was 10% (1/10). The pass rate of microbial sampling was 100% (26/26).ConclusionsThe DFHP had a significant decontamination effect on the ambulance inner uncovered surfaces. The DFHP equipment is automated and their disinfecting quality is consistent, therefore it is suitable for the disinfection of ambulance inner surfaces. But the limitation of disinfection effect on covered surfaces should be avoided.
Infectious cystitis is an inflammatory disease of the bladder wall caused by pathogenic microorganisms invading the urinary tract and is one of the most common types of urinary tract infections in clinical practice. At present, China lacks evidence-based clinical practice guidelines for the integrated diagnosis and treatment of infectious cystitis with Chinese and Western medicine. Consequently, significant variations exist in diagnostic and therapeutic strategies across medical institutions, and the recurrence rate remains relatively high. To further standardize clinical decision-making for patients with infectious cystitis and to promote the translation of the latest clinical research evidence into practice, the working group has initiated the development of the Guidelines on Integrated Chinese and Western Medicine in the Diagnosis and Treatment of Infectious Cystitis. The guideline development will be conducted in accordance with the World Health Organization (WHO) handbook for guideline development, following the standards and requirements of the GRADE system and the Reporting Items for Practice Guidelines in Healthcare (RIGHT). It will also incorporate national health policies, classical medical texts, existing guidelines or consensuses, and the distinctive characteristics of traditional Chinese medicine (TCM) diagnosis and treatment. This proposal systematically outlines the background and significance of the guideline, the composition of the working group, the construction of clinical questions and the identification of outcome indicators, the methodology of evidence collection and systematic evaluation, the formation of recommendations and the consensus process, etc., to provide a methodological basis and technical guidance for the scientific development and standardized release of subsequent guidelines.
The emergence of regular short repetitive palindromic sequence clusters (CRISPR) and CRISPR- associated proteins 9 (Cas9) gene editing technology has greatly promoted the wide application of genetically modified pigs. Efficient single guide RNA (sgRNA) is the key to the success of gene editing using CRISPR/Cas9 technology. For large animals with a long reproductive cycle, such as pigs, it is necessary to screen out efficient sgRNA in vitro to avoid wasting time and resource costs before animal experiments. In addition, how to efficiently obtain positive gene editing monoclonal cells is a difficult problem to be solved. In this study, a rapid sgRNA screening method targeting the pig genome was established and we rapidly obtained Fah gene edited cells, laying a foundation for the subsequent production of Fah knockout pigs as human hepatocyte bioreactor. At the same time, the method of obtaining monoclonal cells using pattern microarray culture technology was explored.
