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The Effect of Atorvastatin on Braindeprived Neurotrophic Factor in Rats with Cerebral Infarction

摘要:目的:觀察阿托伐他丁對腦梗死大鼠腦保護的作用以及對腦源性神經營養因子(braindeprived neurotrophic factor,BDNF)的影響。方法: 線栓法制備SD大鼠大腦中動脈梗死(middle cerebral artery occlusion,MCAO)再灌注模型。將大鼠隨機分為:假手術組;MCAO組的2 h、24 h、3 d、5 d組;阿托伐他丁組的2 h、24 h、3 d、5 d組。MCAO組和阿托伐他丁組的各時程組再分別分為腦梗死體積亞組、免疫組化亞組,每亞組及假手術組各6只大鼠。在不同時間點觀察阿托伐他丁組和MCAO組大鼠神經行為評分、腦梗死體積,用免疫組化法檢測BDNF陽性細胞數。結果: 神經行為評分和腦梗死體積在阿托伐他丁組和MCAO組的2 h組之間無顯著性差異(Pgt;0.05),在阿托伐他丁24 h、3 d、5 d組均顯著低于對應時程的MCAO組(Plt;0.05);各組缺血半暗帶BDNF陽性細胞數均增高,但阿托伐他丁組的陽性細胞數顯著高于對應時程的MCAO組(Plt;0.05)。結論:阿托伐他丁能提高大鼠局灶腦缺血半暗帶BDNF的表達水平,促進神經元的修復。Abstract: Objective: To observe the effect of atorvastatin in cerebral protection and braindeprived neurotrophic factor(BDNF) in rats. Methods: Ischemic reperfusion model of rats as established by an intraluminal filament and recirculation at different time point respectively. One hundred and two healthy SD rats were randomly assigned into three groups for different preconditioning, including the sham surgery group (SS, n=6), the sham and middle cerebralartery occlusion (MCAO) group (MCAO, n=48), and the atorvastatin and MCAO group (atorvastatin +MCAO, n=48). The latter two groups were further divided into two subgroups on different time points of tests. Each subgroup hase six rats. In the atorvastatin +MCAO group, intragastric administration of atorvastatin was given for five days, then the MCAO followed. In the MCAO group, the MCAO was given directly. The neurophysical marks and the volume of the cerebral infarction in atorvastatin group and MCAO group were determined at different time point. The expression of BDNF was valued by immunohistochemitry respectively. Results: At 2 h, there were no differences in the neurophysical marks and volume of the cerebral infarction between atorvastatin group and MCAO group (Pgt;0.05). At 24 h,3 d,5 d, the neurophysical marks and volume of the cerebral infarction of atorvastatin group were lower than that of MCAO group in the corresponding time (Plt;0.05). Around the necrotic areas,BDNF positive neurons were increased in both groups, but they were higher in atorvastatin group than in MCAO group in the corresponding time (Plt;0.05). Conclusion: Atorvastatin could increase the expression level of BDNF and promote the ischemic neuron to revive.