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Sequential Study of the Complement Activation and Cell Apoptosis in Perihematoma tissue in rats

摘要:目的:動態觀察大鼠腦出血后血腫周圍組織補體激活與細胞凋亡的規律。方法:用膠原酶注入到大鼠尾狀核的方法制作腦出血模型。將大鼠分為腦出血、假手術組、正常組3組。采用蘇木素伊紅(HE) 染色、免疫組織化學染色及原位末端脫氧核苷酸轉移酶介導的dUTP 缺口末端標記法(TUNEL)分別觀察各組在腦出血后第6 h、12 h、24 h、48 h、72 h、5 d、7 d時血腫周圍補體C3、促凋亡基因(Bax)、抑凋亡基因(Bclxl)及TUNEL的表達。結果補體C3的表達峰值在24~48 h;TUNEL、Bax蛋白表達術后12h增加,48~72 h達高峰,而Bclxl蛋白表達高峰在48h。結論:大鼠腦出血后血腫周圍組織補體C3的表達增加與細胞凋亡的演變趨勢一致,C3與凋亡有相關。Abstract: Objective: To study the complement activation and apoptosis regular genes changes in the tissues of the perihematoma of intracerebral hemorrhage (ICH) in rats. Methods: Intracerebral hemorrhage was induced in rats by injection of bacterial collagenase into the caudate nucleus. Histopathological changes were studied in 6 h,12 h, 24 h, 2 d, 3 d, 5 d, 7 d after the injection. The immunohistochemistry and TUNEL analysis were performed. The expression of complement factor C3, the TUNELpositive cells, the proapoptotic gene expression (Bax) and the antiapoptotic gene (Bclxl) were examined. Results: The expression of C3 increased to its maximum between 2448 h. The TUNELpositive cells and Bax protein expression increased gradually and reached the peak level between 4872 h. The Bclxl protein reached the peak level at 48 h. The correlation analysis showed that the quantity of C3 was positively related to that of the TUNELpositive cells, but the bax protein was not related to Bclxl protein. Conclusion: The expression of complement factor C3 may contributes to the nerve injury after cerebral hemorrhage and relate to the apotosis in the tissues surrounding the hametoma in rats.