ObjectiveTo verify the consistency between artificial interpretation and automatic interpretation by HELIOS automatic immunofluorescence system by comparing their results on the same antinuclear antibodies (ANA) fluorescent slides, and analyze the application of automatic interpretation clinically.
MethodA total of 281 ANA fluorescent slides of 281 impatients or outpatients in February 2015 were analyzed by HELIOS automatic immunofluorescence system and artificial interpretation respectively. As HELIOS could only determine the titer not the fluorescence type, only the negative or positive results qualitatively and the titer of ANA positive slides were analyzed.
ResultsThere was no statistically significant difference between HELIOS automatic immunofluorescence system and artificial interpretation in negative or positive rate qualitatively (P>0.05) . The total coincidence rate was 98.9%, the positive coincidence rate was 99.5%, and the negative coincidence rate was 97.4%, and the kappa coefficient was 0.973. The difference of titer between the two groups had no statistical significance (P>0.05) .
ConclusionsThe results of HELIOS automatic Immunofluorescence system and artificial interpretation are in good consistency. HELIOS automatic immunofluorescence system is suitable for clinical use as its high degree of automation, simple operation and result reliability.
ObjectiveTo evaluate diagnostic performance of crithidia luciliae immunofluorescence test (CLIFT), enzyme linked immunosorbent assay (ELISA), linear immunoassay (LIA) and chemiluminescence immunoassay (CLIA) for detection of anti-dsDNA antibodies for systemic lupus erythematosus (SLE).
MethodsA total of 178 sera[SLE (n=86), other systemic rheumatic diseases (n=62), and healthy individual (n=30)], from whom received treatment from July 2012 to June 2013, were tested by 4 different assay kits.
ResultsThe diagnostic performances of four methods for detecting anti-dsDNA antibodies for SLE were ELISA, CLIA, CLIFT and LIA, from higher to lower; while ELISA had the highest sensitivity (67.4%), and CLIA had the highest specificity (95.6%). The three test methods (ELISA, LIA, CLIA) had almost perfect concordance with the comparison method (CLIFT, Kappa >0.8). With cut-off values set at 95% of specificity, there was no statistical difference of sensitivity between ELISA and CLIA (58.1%, 60.5%; P>0.05).
ConclusionFour assays can be used for the clinical detection of anti-dsDNA antibodies, and the results have an almost perfect concordance. Different assays show various performances depending on the methods and cut-off values used.
Objective To investigate the clinical features, pathological and immunological manifestations, as well as treatment outcomes in patients with immunoglobulin (Ig) A pemphigus. Methods The clinical, pathological, immunological, and follow-up data of IgA pemphigus patients diagnosed in the Department of Dermatology and Venereology, West China Hospital of Sichuan University between January 1, 2014 and December 31, 2024 were retrospectively analyzed. Results Ultimately, 8 patients were included, including 6 females and 2 males, with an average age of onset of 42.4 years and an average age of medical consultation of 45.3 years. All 8 patients presented with erythema and papules, including 6 cases with yellow white pustules and 2 cases with blisters. The affected areas of the skin lesions included the trunk, limbs, head and neck, and palms and soles, with 4 cases diagnosed with subcorneal pustular dermatosis type IgA pemphigus, 1 case diagnosed with intraepidermal neutrophilic dermatosis type, and the remaining 3 cases had atypical histopathological changes. The 7 successfully followed up patients achieved disease activity control, and during the follow-up period, 5 patients experienced at least 1 recurrence. A total of 6 patients completed itching assessment before and after treatment (3 severe itching and 1 moderate itching), and after treatment, the itching in all patients decreased to mild. Conclusions IgA pemphigus patients often present with blisters and pustules on the trunk and limbs, with a higher incidence of itching. Although most patients have a good response to treatment, recurrence is not uncommon during follow-up.
