Development of a high intensity focused ultrasound device for bio-samples preparation_Journal of Biomedical Engineering

Authors：

LEI Zhubing ¹ , PANG Xinpei ¹ , LI Li ² ,  MEI Qian ^1,2 ,  DONG Wenfei ^1,2

1. School of Biomedical Engineering (Suzhou), University of Science and Technology of China, Suzhou, Jiangsu 215163, P. R. China;
2. Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, P. R. China;

Corresponding?author：

MEI Qian, Email: qmei@sibet.ac.cn; DONG Wenfei, Email: wenfeidong@sibet.ac.cn

Keywords：

Sample fragmentation; High-intensity focused ultrasound; Paraffin-embedded sample

DOI：

10.7507/1001-5515.202304061

Video：

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Abstract Full text Figures/Tables Video References Cited by

A miniaturized, low-cost high-intensity focused ultrasound device is developed for the problems of cross-contamination and uneven sample fragmentation in conventional ultrasound devices. This device generates ultrasonic waves through a concave spherical self-focusing piezoelectric ceramic piece, and creates a cavitation effect in the focusing area to achieve sample fragmentation. The feasibility of the device is demonstrated by physical simulation, then a driving circuit with adjustable power is designed and manufactured to generate 0 ~ 22.4 W acoustic power, and finally paraffin-embedded sample dewaxing experiments are performed to verify the validation of the device. The experimental results show that the dewaxing efficiency and safety of the high-intensity focused ultrasound device is significantly better than those of traditional chemical methods, and this device is comparable with commercial ultrasonic instruments. In summary, the high-intensity focused ultrasound device is expected to be applied in automated nucleic acid extraction and purification equipment and has a broad application prospect in the field of sample pre-processing.

Citation： LEI Zhubing, PANG Xinpei, LI Li, MEI Qian, DONG Wenfei. Development of a high intensity focused ultrasound device for bio-samples preparation. Journal of Biomedical Engineering, 2024, 41(1): 152-159. doi: 10.7507/1001-5515.202304061 Copy

1.	鄒麗華, 閔愛萍, 王惠, 等. 產前超聲檢查對前置胎盤合并胎盤植入的診斷. 影像科學與光化學, 2022, 40(3): 515-519.
2.	黃俐杰, 何瓊, 王銳, 等. 功能超聲成像技術及其應用研究進展. 生物醫學工程學雜志, 2022, 39(5): 1015-1021.
3.	戚曉東, 劉興華, 馮立輝, 等. 多波束超聲技術治療乳腺癌臨床效果的研究. 中國生物醫學工程學報, 2020, 39(4): 504-507.
4.	常詩卉, 王佩國, 菅喜岐. 高強度聚焦超聲腦腫瘤治療焦域溫度均勻分布調控的數值仿真研究. 生物醫學工程學雜志, 2018, 35(6): 877-886.
5.	Gonzalez-Calle A, Li R, Asante I, et al. Development of moderate intensity focused ultrasound (MIFU) for ocular drug delivery. BME Frontiers, 2022, 2022: 9840678.
6.	Fant C, Granzotto A, Mestas J L, et al. DNA double-strand breaks in murine mammary tumor cells induced by combined treatment with doxorubicin and controlled stable cavitation. Ultrasound Med Biol, 2021, 47(10): 2941-2957.
7.	喬龍學, 吳太虎, 陳鋒, 等. 非接觸式超聲裂解系統的設計與優化分析. 儀器儀表學報, 2013, 34(9): 2137-2146.
8.	Holmes H R, Haywood M, Hutchison R, et al. Focused ultrasound extraction (FUSE) for the rapid extraction of DNA from tissue matrices. Methods Ecol Evol, 2020, 11(12): 1599-1608.
9.	Bigelow T A, Xu J, Stessman D J, et al. Lysis of chlamydomonas reinhardtii by high-intensity focused ultrasound as a function of exposure time. Ultrason Sonochem, 2014, 21(3): 1258-1264.
10.	Bomsztyk K, Mar D, Wang Y, et al. PIXUL-ChIP: integrated high-throughput sample preparation and analytical platform for epigenetic studies. Nucleic Acids Res, 2019, 47(12): e69.
11.	桂逢烯, 鄭昊, 李雁浩, 等. 高強度聚焦超聲間歇式治療中焦域溫度分布的仿真研究. 應用聲學, 2021, 40(3): 383-390.
12.	黃家榮, 葉曉靖. 壓電陶瓷電特性測試與分析. 電子技術應用, 2016, 42(8): 16-20.
13.	Svilainis L, Dumbrava V, Chaziachmetovas A. Investigation of the half bridge and transformer push-pull pulser topologies for ultrasonic transducer excitation. J Circuits Syst Comput, 2015, 24(5): 1550062.
14.	Christoffersen C, Wong W, Pichardo S, et al. Class-DE ultrasound transducer driver for HIFU therapy. IEEE Trans Biomed Circuits Syst, 2016, 10(2): 375-382.
15.	Lewis G K, Olbricht W L. Development of a portable therapeutic and high intensity ultrasound system for military, medical, and research use. Rev Sci Instrum, 2008, 79(11): 114302.
16.	Lewis G K, Olbricht W L. Design and characterization of a high-power ultrasound driver with ultralow-output impedance. Rev Sci Instrum, 2009, 80(11): 114704.
17.	Svilainis L, Chaziachmetovas A, Dumbrava V. Half bridge topology 500 V pulser for ultrasonic transducer excitation. Ultrasonics, 2015, 59: 79-85.
18.	Adams C, Carpenter T M, Cowell D, et al. HIFU drive system miniaturization using harmonic reduced pulsewidth modulation. IEEE Trans Ultrason Ferroelectr Freq Control, 2018, 65(12): 2407-2417.
19.	Bui N T, Nguyen T M T, Ataklti G Y, et al. Design of a high-power multilevel sinusoidal signal and high-frequency excitation module based on FPGA for HIFU systems. Electronics, 2021, 10(11): 1299.
20.	Carpenter T M, Cowell D M J, Clegg H R, et al. High-power gallium nitride HIFU transmitter with integrated real-time current and voltage measurement. IEEE Trans Biomed Circuits Syst, 2021, 15(2): 270-280.
21.	Tamano S, Jimbo H, Azuma T, et al. Improvement of high-voltage staircase drive circuit waveform for high-intensity therapeutic ultrasound. JPN J Appl Phys, 2016, 55(7S1): 07KF17.
22.	Tamano S, Yoshizawa S, Umemura S-I. Multifunctional pulse generator for high-intensity focused ultrasound system. JPN J Appl Phys, 2017, 56(7S1): 07JF21.
23.	Tang S C, Clement G T. A harmonic cancellation technique for an ultrasound transducer excited by a switched-mode power converter. IEEE Trans Ultrason Ferroelectr Freq Control, 2008, 55(2): 359-367.
24.	龐宇, 袁鵬飛, 陽妙. 基于DDS的超聲驅動電源系統設計. 現代電子技術, 2020, 43(10): 18-20,24.
25.	張敏, 張元良, 李瑞品. 基于E類功率放大器的雙頻超聲治療系統研究. 醫療衛生裝備, 2019, 40(6): 16-20.
26.	呂葉輝, 李志宏, 劉麗, 等. 人體腦組織福爾馬林固定石蠟包埋樣本中長鏈非編碼RNA的提取及定量分析方法. 上海交通大學學報(醫學版), 2018, 38(4): 400-406.
27.	翁爽, 王明超, 應萬濤, 等. 福爾馬林固定石蠟包埋組織切片蛋白質組分析方法與應用研究進展. 分析測試學報, 2020, 39(1): 75-81.
28.	Bhagwate A V, Liu Y H, Winham S J, et al. Bioinformatics and DNA-extraction strategies to reliably detect genetic variants from FFPE breast tissue samples. BMC Genomics, 2019, 20(1): 689.
29.	Janecka A, Adamczyk A, Gasinska A. Comparison of eight commercially available kits for DNA extraction from formalin-fixed paraffin-embedded tissues. Anal Biochem, 2015, 476: 8-10.
30.	Sarnecka A K, Nawrat D, Piwowar M, et al. DNA extraction from FFPE tissue samples-a comparison of three procedures. Contemp Oncol (Pozn), 2019, 23(1): 52-58.

1. 鄒麗華, 閔愛萍, 王惠, 等. 產前超聲檢查對前置胎盤合并胎盤植入的診斷. 影像科學與光化學, 2022, 40(3): 515-519.
2. 黃俐杰, 何瓊, 王銳, 等. 功能超聲成像技術及其應用研究進展. 生物醫學工程學雜志, 2022, 39(5): 1015-1021.
3. 戚曉東, 劉興華, 馮立輝, 等. 多波束超聲技術治療乳腺癌臨床效果的研究. 中國生物醫學工程學報, 2020, 39(4): 504-507.
4. 常詩卉, 王佩國, 菅喜岐. 高強度聚焦超聲腦腫瘤治療焦域溫度均勻分布調控的數值仿真研究. 生物醫學工程學雜志, 2018, 35(6): 877-886.
5. Gonzalez-Calle A, Li R, Asante I, et al. Development of moderate intensity focused ultrasound (MIFU) for ocular drug delivery. BME Frontiers, 2022, 2022: 9840678.
6. Fant C, Granzotto A, Mestas J L, et al. DNA double-strand breaks in murine mammary tumor cells induced by combined treatment with doxorubicin and controlled stable cavitation. Ultrasound Med Biol, 2021, 47(10): 2941-2957.
7. 喬龍學, 吳太虎, 陳鋒, 等. 非接觸式超聲裂解系統的設計與優化分析. 儀器儀表學報, 2013, 34(9): 2137-2146.
8. Holmes H R, Haywood M, Hutchison R, et al. Focused ultrasound extraction (FUSE) for the rapid extraction of DNA from tissue matrices. Methods Ecol Evol, 2020, 11(12): 1599-1608.
9. Bigelow T A, Xu J, Stessman D J, et al. Lysis of chlamydomonas reinhardtii by high-intensity focused ultrasound as a function of exposure time. Ultrason Sonochem, 2014, 21(3): 1258-1264.
10. Bomsztyk K, Mar D, Wang Y, et al. PIXUL-ChIP: integrated high-throughput sample preparation and analytical platform for epigenetic studies. Nucleic Acids Res, 2019, 47(12): e69.
11. 桂逢烯, 鄭昊, 李雁浩, 等. 高強度聚焦超聲間歇式治療中焦域溫度分布的仿真研究. 應用聲學, 2021, 40(3): 383-390.
12. 黃家榮, 葉曉靖. 壓電陶瓷電特性測試與分析. 電子技術應用, 2016, 42(8): 16-20.
13. Svilainis L, Dumbrava V, Chaziachmetovas A. Investigation of the half bridge and transformer push-pull pulser topologies for ultrasonic transducer excitation. J Circuits Syst Comput, 2015, 24(5): 1550062.
14. Christoffersen C, Wong W, Pichardo S, et al. Class-DE ultrasound transducer driver for HIFU therapy. IEEE Trans Biomed Circuits Syst, 2016, 10(2): 375-382.
15. Lewis G K, Olbricht W L. Development of a portable therapeutic and high intensity ultrasound system for military, medical, and research use. Rev Sci Instrum, 2008, 79(11): 114302.
16. Lewis G K, Olbricht W L. Design and characterization of a high-power ultrasound driver with ultralow-output impedance. Rev Sci Instrum, 2009, 80(11): 114704.
17. Svilainis L, Chaziachmetovas A, Dumbrava V. Half bridge topology 500 V pulser for ultrasonic transducer excitation. Ultrasonics, 2015, 59: 79-85.
18. Adams C, Carpenter T M, Cowell D, et al. HIFU drive system miniaturization using harmonic reduced pulsewidth modulation. IEEE Trans Ultrason Ferroelectr Freq Control, 2018, 65(12): 2407-2417.
19. Bui N T, Nguyen T M T, Ataklti G Y, et al. Design of a high-power multilevel sinusoidal signal and high-frequency excitation module based on FPGA for HIFU systems. Electronics, 2021, 10(11): 1299.
20. Carpenter T M, Cowell D M J, Clegg H R, et al. High-power gallium nitride HIFU transmitter with integrated real-time current and voltage measurement. IEEE Trans Biomed Circuits Syst, 2021, 15(2): 270-280.
21. Tamano S, Jimbo H, Azuma T, et al. Improvement of high-voltage staircase drive circuit waveform for high-intensity therapeutic ultrasound. JPN J Appl Phys, 2016, 55(7S1): 07KF17.
22. Tamano S, Yoshizawa S, Umemura S-I. Multifunctional pulse generator for high-intensity focused ultrasound system. JPN J Appl Phys, 2017, 56(7S1): 07JF21.
23. Tang S C, Clement G T. A harmonic cancellation technique for an ultrasound transducer excited by a switched-mode power converter. IEEE Trans Ultrason Ferroelectr Freq Control, 2008, 55(2): 359-367.
24. 龐宇, 袁鵬飛, 陽妙. 基于DDS的超聲驅動電源系統設計. 現代電子技術, 2020, 43(10): 18-20,24.
25. 張敏, 張元良, 李瑞品. 基于E類功率放大器的雙頻超聲治療系統研究. 醫療衛生裝備, 2019, 40(6): 16-20.
26. 呂葉輝, 李志宏, 劉麗, 等. 人體腦組織福爾馬林固定石蠟包埋樣本中長鏈非編碼RNA的提取及定量分析方法. 上海交通大學學報(醫學版), 2018, 38(4): 400-406.
27. 翁爽, 王明超, 應萬濤, 等. 福爾馬林固定石蠟包埋組織切片蛋白質組分析方法與應用研究進展. 分析測試學報, 2020, 39(1): 75-81.
28. Bhagwate A V, Liu Y H, Winham S J, et al. Bioinformatics and DNA-extraction strategies to reliably detect genetic variants from FFPE breast tissue samples. BMC Genomics, 2019, 20(1): 689.
29. Janecka A, Adamczyk A, Gasinska A. Comparison of eight commercially available kits for DNA extraction from formalin-fixed paraffin-embedded tissues. Anal Biochem, 2015, 476: 8-10.
30. Sarnecka A K, Nawrat D, Piwowar M, et al. DNA extraction from FFPE tissue samples-a comparison of three procedures. Contemp Oncol (Pozn), 2019, 23(1): 52-58.

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Development of a high intensity focused ultrasound device for bio-samples preparation

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