組織工程雙相支架修復關節軟骨及軟骨下骨缺損研究進展_West China Medical Journal

Authors：

 劉明 ¹ , 余曦 ² , 黃富國 ¹ , 岑石強 ¹ ,  項舟 ¹

1. ;
2. ;

Corresponding?author：

項舟, Email: xiangzhou15@hotmail.com

Keywords：

組織工程; 干細胞; 骨軟骨缺損; 雙相支架; 修復

DOI：

10.7507/1002-0179.20140238

Video：

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Abstract Full text Figures/Tables Video References Cited by

通過回顧近年來國內外采用的組織工程雙相支架修復軟骨及軟骨下骨缺損的文獻，比較不同雙相支架修復軟骨及軟骨下骨效果差別，分析其優勢及存在的主要問題，并初步探討軟骨修復的合理方式和發展趨勢。研究結果表明，以雙相支架作為載體材料一體化修復軟骨及軟骨下骨缺損，較之傳統方式以及其他組織工程修復途徑有著不可比擬的優越性，而復合理想的種子細胞及合適生長因子后，更能促進修復效果。由此可見，雙相支架有利于修復骨軟骨缺損，而構建仿生化多相支架是未來研究的趨勢。

Citation： 劉明, 余曦, 黃富國, 岑石強, 項舟. 組織工程雙相支架修復關節軟骨及軟骨下骨缺損研究進展. West China Medical Journal, 2014, 29(4): 783-787. doi: 10.7507/1002-0179.20140238 Copy

1.	Qi YY, Chen X, Jiang YZ, et al. Local delivery of autologous platelet in collagen matrix simulated in situ articular cartilage repair[J]. Cell Transplant, 2009, 18(10):1161-1169.
2.	Chen WC, Yao CL, Wei YH, et al. Evaluating osteochondral defect repair potential of autologous rabbit bone marrow cells on type Ⅱ collagen scaffold[J]. Cytotechnology, 2011, 63(1):13-23.
3.	Fritz J, Janssen P, Gaissmaier C, et al. Articular cartilage defects in the knee——basics, therapies and results[J]. Injury, 2008, 39(Suppl 1):S50-S57.
4.	Swieszkowski W, Tuan BH, Kurzydlowski KJ, et al. Repair and regeneration of osteochondral defects in the articular joints[J]. Biomol Eng, 2007, 24(5):489-495.
5.	王秋玲, 呂玉明, 謝德明. 組織工程修復骨及軟骨缺損的現狀及展望[J]. 中國組織工程研究與臨床康復, 2009, 13(37):7358-7362.
6.	Haasper C, Zeichen J, Meister R, et al. Tissue engineering of osteochondral constructs in vitro using bioreactors[J]. Injury, 2008, 39(Suppl 1):S66-S76.
7.	Schek RM, Taboas JM, Segvich SJ, et al. Engineered osteochondral grafts using biphasic composite solid free-form fabricated scaffolds[J]. Tissue Eng, 2004, 10(9/10):1376-1385.
8.	Martin I, Miot S, Barbero A, et al. Osteochondral tissue engineering[J]. J Biomech, 2007, 40(4):750-765.
9.	Holland TA, Bodde EW, Baggett LS, et al. Osteochondral repair in the rabbit model utilizing bilayered, degradable oligo[poly (ethylene glycol) fumarate] hydrogel scaffolds[J]. J Biomed Mater Res A, 2005, 75(1):156-167.
10.	Getgood AM, Kew SJ, Brooks R, et al. Evaluation of early-stage osteochondral defect repair using a biphasic scaffold based on a collagen-glycosaminoglycan biopolymer in a caprine model[J]. Knee, 2012, 19(4):422-430.
11.	Jiang CC, Chiang H, Liao CJ, et al. Repair of porcine articular cartilage defect with a biphasic osteochondral composite[J]. J Orthop Res, 2007, 25(10):1277-1290.
12.	Liljensten E, Adolfsson E, Strid KG, et al. Resorbable and nonresorbable hydroxyapatite granules as bone graft substitutes in rabbit cortical defects[J]. Clin Implant Dent Relat Res, 2003, 5(2):95-101.
13.	Jurgens WJ, Kroeze RJ, Zandieh-Doulabi B, et al. One-step surgical procedure for the treatment of osteochondral defects with adipose-derived stem cells in a caprine knee defect:a pilot study[J]. Biores Open Access, 2013, 2(4):315-325.
14.	Gao J, Dennis JE, Solchaga LA, et al. Repair of osteochondral defect with tissue-engineered two-phase composite material of injectable Calcium phosphate and hyaluronan sponge[J]. Tissue Eng, 2002, 8(5):827-837.
15.	Ho ST, Hutmacher DW, Ekaputra AK, et al. The evaluation of a biphasic osteochondral implant coupled with an electrospun membrane in a large animal model[J]. Tissue Eng Part A, 2010, 16(4):1123-1141.
16.	Pei M, He F, Boyce BM, et al. Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs[J]. Osteoarthritis Cartilage, 2009, 17(6):714-722.
17.	張華亮, 王文良, 初殿偉, 等. 雙層殼聚糖與HAP復合支架的初步研究[J]. 中國修復重建外科雜志, 2008, 22(11):1358-1363.
18.	Shao XX, Hutmacher DW, Ho ST, et al. Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits[J]. Biomaterials, 2006, 27(7):1071-1080.
19.	Arien-Zakay H, Lazarovici P, Nagler A. Tissue regeneration potential in human umbilical cord blood[J]. Best Pract Res Clin Haematol, 2010, 23(2):291-303.
20.	Rodrigues MT, Lee SJ, Gomes ME, et al. Bilayered constructs aimed at osteochondral strategies:the influence of medium supplements in the osteogenic and chondrogenic differentiation of amniotic fluid-derived stem cells[J]. Acta Biomater, 2012, 8(7):2795-2806.
21.	Im GI, Ahn JH, Kim SY, et al. A hyaluronate-atelocollagen/beta-tricalcium phosphate-hydroxyapatite biphasic scaffold for the repair of osteochondral defects:a porcine study[J]. Tissue Eng Part A, 2010, 16(4):1189-1200.
22.	Yang HN, Park JS, Woo DG, et al. Chondrogenesis of mesenchymal stem cells and dedifferentiated chondrocytes by transfection with SOX Trio genes[J]. Biomaterials, 2011, 32(30):7695-7704.
23.	Lam J, Lu S, Meretoja VV, et al. Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels[J]. Acta Biomater, 2014, 10(3):1112-1123.
24.	Li WJ, Chiang H, Kuo TF, et al. Evaluation of articular cartilage repair using biodegradable nanofibrous scaffolds in a swine model:a pilot study[J]. J Tissue Eng Regen Med, 2009, 3(1):1-10.
25.	Sakata R, Kokubu T, Nagura I, et al. Localization of vascular endothelial growth factor during the early stages of osteochondral regeneration using a bioabsorbable synthetic polymer scaffold[J]. J Orthop Res, 2012, 30(2):252-259.
26.	Chimal-Monroy J, Díaz de León L. Differential effects of transforming growth factors beta 1, beta 2, beta 3 and beta 5 on chondrogenesis in mouse limb bud mesenchymal cells[J]. Int J Dev Biol, 1997, 41(1):91-102.
27.	Hunziker EB. Articular cartilage repair:are the intrinsic biological constraints undermining this process insuperable?[J]. Osteoarthritis Cartilage, 1999, 7(1):15-28.
28.	Minas T. The role of cartilage repair techniques, including chondrocyte transplantation, in focal chondral knee damage[J]. Instr Course Lect, 1999, 48:629-643.
29.	Jackson DW, Simon TM. Tissue engineering principles in orthopaedic surgery[J]. Clin Orthop Relat Res, 1999(367 Suppl):S31-S45.
30.	Vangsness CT, Kurzweil PR, Lieberman JR. Restoring articular cartilage in the knee[J]. Am J Orthop (Belle Mead NJ), 2004, 33(2 Supp2):29-34.
31.	Driesang IM, Hunziker EB. Delamination rates of tissue flaps used in articular cartilage repair[J]. J Orthop Res, 2000, 18(6):909-911.
32.	Guo X, Wang C, Duan C, et al. Repair of osteochondral defects with autologous chondrocytes seeded onto bioceramic scaffold in sheep[J]. Tissue Eng, 2005, 10(11/12):1830-1840.
33.	Wang X, Grogan SP, Rieser F, et al. Tissue engineering of biphasic cartilage constructs using various biodegradable scaffolds:an in vitro study[J]. Biomaterials, 2004, 25(17):3681-3688.
34.	Dormer NH, Berkland CJ, Detamore MS. Emerging techniques in stratified designs and continuous gradients for tissue engineering of interfaces[J]. Ann Biomed Eng, 2010, 38(6):2121-2141.
35.	Kon E, Delcogliano M, Filardo G, et al. Novel nano-composite multilayered biomaterial for osteochondral regeneration:a pilot clinical trial[J]. Am J Sports Med, 2011, 39(6):1180-1190.
36.	Kon E, Delcogliano M, Filardo G, et al. Novel nano-composite multi-layered biomaterial for the treatment of multifocal degenerative cartilage lesions[J]. Knee Surg Sports Traumatol Arthrosc, 2009, 17(11):1312-1315.
37.	Kon E, Delcogliano M, Filardo G, et al. A novel nano-composite multi-layered biomaterial for treatment of osteochondral lesions:technique note and an early stability pilot clinical trial[J]. Injury, 2010, 41(7):693-701.
38.	Mohan N, Dormer NH, Caldwell KL, et al. Continuous gradients of material composition and growth factors for effective regeneration of the osteochondral interface[J]. Tissue Eng Part A, 2011, 17(21/22):2845-2855.
39.	Liu C, Han Z, Czernuszka JT. Gradient collagen/nanohydroxyapatite composite scaffold:development and characterization[J]. Acta Biomater, 2009, 5(2):661-669.

1. Qi YY, Chen X, Jiang YZ, et al. Local delivery of autologous platelet in collagen matrix simulated in situ articular cartilage repair[J]. Cell Transplant, 2009, 18(10):1161-1169.
2. Chen WC, Yao CL, Wei YH, et al. Evaluating osteochondral defect repair potential of autologous rabbit bone marrow cells on type Ⅱ collagen scaffold[J]. Cytotechnology, 2011, 63(1):13-23.
3. Fritz J, Janssen P, Gaissmaier C, et al. Articular cartilage defects in the knee——basics, therapies and results[J]. Injury, 2008, 39(Suppl 1):S50-S57.
4. Swieszkowski W, Tuan BH, Kurzydlowski KJ, et al. Repair and regeneration of osteochondral defects in the articular joints[J]. Biomol Eng, 2007, 24(5):489-495.
5. 王秋玲, 呂玉明, 謝德明. 組織工程修復骨及軟骨缺損的現狀及展望[J]. 中國組織工程研究與臨床康復, 2009, 13(37):7358-7362.
6. Haasper C, Zeichen J, Meister R, et al. Tissue engineering of osteochondral constructs in vitro using bioreactors[J]. Injury, 2008, 39(Suppl 1):S66-S76.
7. Schek RM, Taboas JM, Segvich SJ, et al. Engineered osteochondral grafts using biphasic composite solid free-form fabricated scaffolds[J]. Tissue Eng, 2004, 10(9/10):1376-1385.
8. Martin I, Miot S, Barbero A, et al. Osteochondral tissue engineering[J]. J Biomech, 2007, 40(4):750-765.
9. Holland TA, Bodde EW, Baggett LS, et al. Osteochondral repair in the rabbit model utilizing bilayered, degradable oligo[poly (ethylene glycol) fumarate] hydrogel scaffolds[J]. J Biomed Mater Res A, 2005, 75(1):156-167.
10. Getgood AM, Kew SJ, Brooks R, et al. Evaluation of early-stage osteochondral defect repair using a biphasic scaffold based on a collagen-glycosaminoglycan biopolymer in a caprine model[J]. Knee, 2012, 19(4):422-430.
11. Jiang CC, Chiang H, Liao CJ, et al. Repair of porcine articular cartilage defect with a biphasic osteochondral composite[J]. J Orthop Res, 2007, 25(10):1277-1290.
12. Liljensten E, Adolfsson E, Strid KG, et al. Resorbable and nonresorbable hydroxyapatite granules as bone graft substitutes in rabbit cortical defects[J]. Clin Implant Dent Relat Res, 2003, 5(2):95-101.
13. Jurgens WJ, Kroeze RJ, Zandieh-Doulabi B, et al. One-step surgical procedure for the treatment of osteochondral defects with adipose-derived stem cells in a caprine knee defect:a pilot study[J]. Biores Open Access, 2013, 2(4):315-325.
14. Gao J, Dennis JE, Solchaga LA, et al. Repair of osteochondral defect with tissue-engineered two-phase composite material of injectable Calcium phosphate and hyaluronan sponge[J]. Tissue Eng, 2002, 8(5):827-837.
15. Ho ST, Hutmacher DW, Ekaputra AK, et al. The evaluation of a biphasic osteochondral implant coupled with an electrospun membrane in a large animal model[J]. Tissue Eng Part A, 2010, 16(4):1123-1141.
16. Pei M, He F, Boyce BM, et al. Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs[J]. Osteoarthritis Cartilage, 2009, 17(6):714-722.
17. 張華亮, 王文良, 初殿偉, 等. 雙層殼聚糖與HAP復合支架的初步研究[J]. 中國修復重建外科雜志, 2008, 22(11):1358-1363.
18. Shao XX, Hutmacher DW, Ho ST, et al. Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits[J]. Biomaterials, 2006, 27(7):1071-1080.
19. Arien-Zakay H, Lazarovici P, Nagler A. Tissue regeneration potential in human umbilical cord blood[J]. Best Pract Res Clin Haematol, 2010, 23(2):291-303.
20. Rodrigues MT, Lee SJ, Gomes ME, et al. Bilayered constructs aimed at osteochondral strategies:the influence of medium supplements in the osteogenic and chondrogenic differentiation of amniotic fluid-derived stem cells[J]. Acta Biomater, 2012, 8(7):2795-2806.
21. Im GI, Ahn JH, Kim SY, et al. A hyaluronate-atelocollagen/beta-tricalcium phosphate-hydroxyapatite biphasic scaffold for the repair of osteochondral defects:a porcine study[J]. Tissue Eng Part A, 2010, 16(4):1189-1200.
22. Yang HN, Park JS, Woo DG, et al. Chondrogenesis of mesenchymal stem cells and dedifferentiated chondrocytes by transfection with SOX Trio genes[J]. Biomaterials, 2011, 32(30):7695-7704.
23. Lam J, Lu S, Meretoja VV, et al. Generation of osteochondral tissue constructs with chondrogenically and osteogenically predifferentiated mesenchymal stem cells encapsulated in bilayered hydrogels[J]. Acta Biomater, 2014, 10(3):1112-1123.
24. Li WJ, Chiang H, Kuo TF, et al. Evaluation of articular cartilage repair using biodegradable nanofibrous scaffolds in a swine model:a pilot study[J]. J Tissue Eng Regen Med, 2009, 3(1):1-10.
25. Sakata R, Kokubu T, Nagura I, et al. Localization of vascular endothelial growth factor during the early stages of osteochondral regeneration using a bioabsorbable synthetic polymer scaffold[J]. J Orthop Res, 2012, 30(2):252-259.
26. Chimal-Monroy J, Díaz de León L. Differential effects of transforming growth factors beta 1, beta 2, beta 3 and beta 5 on chondrogenesis in mouse limb bud mesenchymal cells[J]. Int J Dev Biol, 1997, 41(1):91-102.
27. Hunziker EB. Articular cartilage repair:are the intrinsic biological constraints undermining this process insuperable?[J]. Osteoarthritis Cartilage, 1999, 7(1):15-28.
28. Minas T. The role of cartilage repair techniques, including chondrocyte transplantation, in focal chondral knee damage[J]. Instr Course Lect, 1999, 48:629-643.
29. Jackson DW, Simon TM. Tissue engineering principles in orthopaedic surgery[J]. Clin Orthop Relat Res, 1999(367 Suppl):S31-S45.
30. Vangsness CT, Kurzweil PR, Lieberman JR. Restoring articular cartilage in the knee[J]. Am J Orthop (Belle Mead NJ), 2004, 33(2 Supp2):29-34.
31. Driesang IM, Hunziker EB. Delamination rates of tissue flaps used in articular cartilage repair[J]. J Orthop Res, 2000, 18(6):909-911.
32. Guo X, Wang C, Duan C, et al. Repair of osteochondral defects with autologous chondrocytes seeded onto bioceramic scaffold in sheep[J]. Tissue Eng, 2005, 10(11/12):1830-1840.
33. Wang X, Grogan SP, Rieser F, et al. Tissue engineering of biphasic cartilage constructs using various biodegradable scaffolds:an in vitro study[J]. Biomaterials, 2004, 25(17):3681-3688.
34. Dormer NH, Berkland CJ, Detamore MS. Emerging techniques in stratified designs and continuous gradients for tissue engineering of interfaces[J]. Ann Biomed Eng, 2010, 38(6):2121-2141.
35. Kon E, Delcogliano M, Filardo G, et al. Novel nano-composite multilayered biomaterial for osteochondral regeneration:a pilot clinical trial[J]. Am J Sports Med, 2011, 39(6):1180-1190.
36. Kon E, Delcogliano M, Filardo G, et al. Novel nano-composite multi-layered biomaterial for the treatment of multifocal degenerative cartilage lesions[J]. Knee Surg Sports Traumatol Arthrosc, 2009, 17(11):1312-1315.
37. Kon E, Delcogliano M, Filardo G, et al. A novel nano-composite multi-layered biomaterial for treatment of osteochondral lesions:technique note and an early stability pilot clinical trial[J]. Injury, 2010, 41(7):693-701.
38. Mohan N, Dormer NH, Caldwell KL, et al. Continuous gradients of material composition and growth factors for effective regeneration of the osteochondral interface[J]. Tissue Eng Part A, 2011, 17(21/22):2845-2855.
39. Liu C, Han Z, Czernuszka JT. Gradient collagen/nanohydroxyapatite composite scaffold:development and characterization[J]. Acta Biomater, 2009, 5(2):661-669.

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組織工程雙相支架修復關節軟骨及軟骨下骨缺損研究進展

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