角質形成細胞的機械應力傳導通路研究進展_《中國修復重建外科雜志》

作者：

付思祺 , 范金財

中國醫學科學院整形外科醫院北三病房整形九科（北京，100144）;

關鍵詞：

角質形成細胞機械應力應力傳導應力感受

DOI：

10.7507/1002-1892.20130113

視頻：

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摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的總結近年來有關角質形成細胞機械應力的感受及傳導作用分子、信號通路研究進展，為促進傷口愈合及加快組織擴張尋找新的途徑。方法查閱近年國內外關于角質形成細胞機械應力的感受和傳導作用分子、信號通路的相關文獻，進行總結分析。結果角質形成細胞機械應力感受機制包括機械應力敏感通道、生長因子介導的機械應力感受和蛋白形變介導的機械應力感受，機械應力傳導的信號通路有細胞黏附介導的信號通路、促分裂原活化蛋白激酶信號通路和細胞骨架、細胞外基質等。結論角質形成細胞可以感受機械應力，并通過不同信號通路傳導有效刺激信息，完成形變、遷移、增殖或分化等一系列生物學行為，以調節適應新的環境。

引用本文： 付思祺,范金財. 角質形成細胞的機械應力傳導通路研究進展. 中國修復重建外科雜志, 2013, 27(4): 500-506. doi: 10.7507/1002-1892.20130113 復制

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1. Turner CH, Owan I, Takano Y. Mechanotransduction in bone: role of strain rate. Am J Physiol, 1995, 269(3 Pt 1): E438-442.
2. Orr AW, Helmke BP, Blackman BR, et al. Mechanisms of mechanotransduction. Dev Cell, 2006, 10(1): 11-20.
3. Matsubayashi Y, Ebisuya M, Honjoh S, et al. ERK activation propagates in epithelial cell sheets and regulates their migration during wound healing. Curr Biol, 2004, 14(8): 731-735.
4. Martinac B. Mechanosensitive ion channels: molecules of mechanotransduction. J Cell Sci, 2004, 117(Pt 12): 2449-2460.
5. Yano S, Komine M, Fujimoto M, et al. Activation of Akt by mechanical stretching in human epidermal keratinocytes. Exp Dermatol, 2006, 15(5): 356-361.
6. Takei T, Han O, Ikeda M, et al. Cyclic strain stimulates isoform-specific PKC activation and translocation in cultured human keratinocytes. J Cell Biochem, 1997, 67(3): 327-337.
7. Moqrich A, Hwang SW, Earley TJ, et al. Impaired thermosensation in mice lacking TRPV3, a heat and camphor sensor in the skin. Science, 2005, 307(5714): 1468-1472.
8. O’Neil RG, Heller S. The mechanosensitive nature of TRPV channels. Pflugers Arch, 2005, 451(1): 193-203.
9. Evans WH, De Vuyst E, Leybaert L. The gap junction cellular internet: connexin hemichannels enter the signalling limelight. Biochem J, 2006, 397(1): 1-14.
10. Jiang JX, Siller-Jackson AJ, Burra S. Roles of gap junctions and hemichannels in bone cell functions and in signal transmission of mechanical stress. Front Biosci, 2007, 12: 1450-1462.
11. Hennings H, Michael D, Cheng C, et al. Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell, 1980, 19(1): 245-254.
12. Wilson E, Mai Q, Sudhir K, et al. Mechanical strain induces growth of vascular smooth muscle cells via autocrine action of PDGF. J Cell Biol, 1993, 123(3): 741-747.
13. Zhang B, Peng F, Wu D, et al. Caveolin-1 phosphorylation is required for stretch-induced EGFR and Akt activation in mesangial cells. Cell Signal, 2007, 19(8): 1690-1700.
14. Knies Y, Bernd A, Kaufmann R, et al. Mechanical stretch induces clustering of beta1-integrins and facilitates adhesion. Exp Dermatol, 2006, 15(5): 347-355.
15. Vogel V. Mechanotransduction involving multimodular proteins: converting force into biochemical signals. Annu Rev Biophys Biomol Struct, 2006, 35: 459-488.
16. Kung C. A possible unifying principle for mechanosensation. Nature, 2005, 436(7051): 647-654.
17. Vogel V, Sheetz M. Local force and geometry sensing regulate cell functions. Nat Rev Mol Cell Biol, 2006, 7(4): 265-275.
18. Katz BZ, Zamir E, Bershadsky A, et al. Physical state of the extracellular matrix regulates the structure and molecular composition of cell-matrix adhesions. Mol Biol Cell, 2000, 11(3): 1047-1060.
19. Tamada M, Sheetz MP, Sawada Y. Activation of a signaling cascade by cytoskeleton stretch. Dev Cell, 2004, 7(5): 709-718.
20. Sawada Y, Tamada M, Dubin-Thaler BJ, et al. Force sensing by mechanical extension of the Src family kinase substrate p130Cas. Cell, 2006, 127(5): 1015-1026.
21. Sawada Y, Nakamura K, Doi K, et al. Rap1 is involved in cell stretching modulation of p38 but not ERK or JNK MAP kinase. J Cell Sci, 2001, 114(Pt 6): 1221-1227.
22. Lee G, Abdi K, Jiang Y, et al. Nanospring behaviour of ankyrin repeats. Nature, 2006, 440(7081): 246-249.
23. Ortiz V, Nielsen SO, Klein ML, et al. Unfolding a linker between helical repeats. J Mol Biol, 2005, 349(3): 638-647.
24. Craig D, Krammer A, Schulten K, et al. Comparison of the early stages of forced unfolding for fibronectin type III modules. Proc Natl Acad Sci U S A, 2001, 98(10): 5590-5595.
25. Gao M, Craig D, Vogel V, et al. Identifying unfolding intermediates of FN-III(10) by steered molecular dynamics. J Mol Biol, 2002, 323(5): 939-950.
26. Geiger B, Bershadsky A. Exploring the neighborhood: adhesion-coupled cell mechanosensors. Cell, 2002, 110(2): 139-142.
27. Zhong C, Chrzanowska-Wodnicka M, Brown J, et al. Rho-mediated contractility exposes a cryptic site in fibronectin and induces fibronectin matrix assembly. J Cell Biol, 1998, 141(2): 539-551.
28. Rubin CT, Lanyon LE. Dynamic strain similarity in vertebrates; an alternative to allometric limb bone scaling. J Theor Biol, 1984, 107(2): 321-327.
29. Shirinsky VP, Antonov AS, Birukov KG, et al. Mechano-chemical control of human endothelium orientation and size. J Cell Biol, 1989, 109(1): 331-339.
30. Takemasa T, Yamaguchi T, Yamamoto Y, et al. Oblique alignment of stress fibers in cells reduces the mechanical stress in cyclically deforming fields. Eur J Cell Biol, 1998, 77(2): 91-99.
31. Hofmann M, Zaper J, Bernd A, et al. Mechanical pressure-induced phosphorylation of p38 mitogen-activated protein kinase in epithelial cells via Src and protein kinase C. Biochem Biophys Res Commun, 2004, 316(3): 673-679.
32. Görmar FE, Bernd A, Bereiter-Hahn J, et al. A new model of epidermal differentiation: induction by mechanical stimulation. Arch Dermatol Res, 1990, 282(1): 22-32.
33. Swensson O, Langbein L, McMillan JR, et al. Specialized keratin expression pattern in human ridged skin as an adaptation to high physical stress. Br J Dermatol, 1998, 139(5): 767-775.
34. Felsenfeld DP, Schwartzberg PL, Venegas A, et al. Selective regulation of integrin—cytoskeleton interactions by the tyrosine kinase Src. Nat Cell Biol, 1999, 1(4): 200-206.
35. Wang JH, Thampatty BP, Lin JS, et al. Mechanoregulation of gene expression in fibroblasts. Gene, 2007, 391(1-2): 1-15.
36. Cukierman E, Pankov R, Stevens DR, et al. Taking cell-matrix adhesions to the third dimension. Science, 2001, 294(5547): 1708-1712.
37. Reichelt J. Mechanotransduction of keratinocytes in culture and in the epidermis. Eur J Cell Biol, 2007, 86(11-12): 807-816.
38. Katsumi A, Orr AW, Tzima E, et al. Integrins in mechanotransduction. J Biol Chem, 2004, 279(13): 12001-12004.
39. Turchi L, Chassot AA, Bourget I, et al. Cross-talk between RhoGTPases and stress activated kinases for matrix metalloproteinase-9 induction in response to keratinocytes injury. J Invest Dermatol, 2003, 121(6): 1291-1300.
40. Burridge K, Fath K. Focal contacts: transmembrane links between the extracellular matrix and the cytoskeleton. Bioessays, 1989, 10(4): 104-108.
41. Okuda M, Takahashi M, Suero J, et al. Shear stress stimulation of p130(cas) tyrosine phosphorylation requires calcium-dependent c-Src activation. J Biol Chem, 1999, 274(38): 26803-26809.
42. Roovers K, Assoian RK. Effects of rho kinase and actin stress fibers on sustained extracellular signal-regulated kinase activity and activation of G(1) phase cyclin-dependent kinases. Mol Cell Biol, 2003, 23(12): 4283-4294.
43. Jalali S, del Pozo MA, Chen K, et al. Integrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix (ECM) ligands. Proc Natl Acad Sci U S A, 2001, 98(3): 1042-1046.
44. Katsumi A, Naoe T, Matsushita T, et al. Integrin activation and matrix binding mediate cellular responses to mechanical stretch. J Biol Chem, 2005, 280(17): 16546-16549.
45. Shemesh T, Geiger B, Bershadsky AD, et al. Focal adhesions as mechanosensors: a physical mechanism. Proc Natl Acad Sci U S A, 2005, 102(35): 12383-12388.
46. Tzima E, Irani-Tehrani M, Kiosses WB, et al. A mechanosensory complex that mediates the endothelial cell response to fluid shear stress. Nature, 2005, 437(7057): 426-431.
47. Bershadsky AD, Balaban NQ, Geiger B. Adhesion-dependent cell mechanosensitivity. Annu Rev Cell Dev Biol, 2003, 19: 677-695.
48. Takei T, Rivas-Gotz C, Delling CA, et al. Effect of strain on human keratinocytes in vitro. J Cell Physiol, 1997, 173(1): 64-72.
49. Yano S, Komine M, Fujimoto M, et al. Mechanical stretching in vitro regulates signal transduction pathways and cellular proliferation in human epidermal keratinocytes. J Invest Dermatol, 2004, 122(3): 783-790.
50. Takei T, Kito H, Du W, et al. Induction of interleukin (IL)-1 alpha and beta gene expression in human keratinocytes exposed to repetitive strain: their role in strain-induced keratinocyte proliferation and morphological change. J Cell Biochem, 1998, 69(2): 95-103.
51. Nguyen HT, Adam RM, Bride SH, et al. Cyclic stretch activates p38 SAPK2-, ErbB2-, and AT1-dependent signaling in bladder smooth muscle cells. Am J Physiol Cell Physiol, 2000, 279(4): C1155-1167.
52. Kippenberger S, Loitsch S, Guschel M, et al. Mechanical stretch stimulates protein kinase B/Akt phosphorylation in epidermal cells via angiotensin II type 1 receptor and epidermal growth factor receptor. J Biol Chem, 2005, 280(4): 3060-3067.
53. Zou Y, Akazawa H, Qin Y, et al. Mechanical stress activates angiotensin II type 1 receptor without the involvement of angiotensin II. Nat Cell Biol, 2004, 6(6): 499-506.
54. Cabodi S, Moro L, Bergatto E, et al. Integrin regulation of epidermal growth factor (EGF) receptor and of EGF-dependent responses. Biochem Soc Trans, 2004, 32(Pt3): 438-442.
55. Daub H, Weiss FU, Wallasch C, et al. Role of transactivation of the EGF receptor in signalling by G-protein-coupled receptors. Nature, 1996, 379(6565): 557-560.
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