IMMUNOGENICITY OF ALLOGENEIC FREEZING PERIOSTEUM AND BONE MARROW_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

DENGJiuzheng ¹ ,  ZHANGYoule ² , LIXiang ³

1. Jishuitan College of Orthopedic Surgery, Medical Center of Tsinghua University, Beijing, 100091, P. R. China;
2. Department of Hand Surgery, Beijing Jishuitan Hospital;
3. Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong;

Corresponding?author：

ZHANGYoule, Email: zhangyoule2007@hotmail.com

Keywords：

Allograft; Immunogenicity; Periosteum; Bone marrow; Rabbit

DOI：

10.7507/1002-1892.20140143

Video：

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Objective To investigate the immunogenicity of freezing periosteum and bone marrow during allogeneic joint transplantation, and to explore proper pretreatment of allogeneic joint. Methods The allogeneic periosteum and bone marrow were harvested from knee joints of 5 New Zealand white rabbits (aged, 6 months; weighing, 2.6-3.0 kg). After gradient cooling, the tissue was cryopreserved for 1 month. The freezing periosteum and bone marrow were grinded to pieces after rewarming to prepare the suspension of periosteum and bone marrow. Eighteen Chinchilla rabbits (aged, 6 months; weighing, 2.1-2.8 kg) were divided into 3 groups randomly:normal saline injection group (group A, n=6), periosteum injection group (group B, n=6), and bone marrow injection group (group C, n=6). The normal saline, periosteum suspension, and bone marrow suspension were injected into the peritoneal cavity in groups A, B, and C, respectively. The concentrations of interleukin 2 (IL-2), IL-6, and tumor necrosis factor α (TNF-α) in serum and the ratio of CD4⁺ T cell/CD8⁺ T cell in venous blood were measured before injection, at 1 week and 2 weeks after injection. Results There was no significant difference in the concentration of IL-2 between before and after injection in the same group (P=0.241), and between groups (P=0.055). The concentration of IL-6 after injection was significantly lower than that before injection in the same group (P=0.040), but no significant difference was found between groups (P=0.357). The concentration of TNF-α showed no significant difference between before and after injection in the same group (P=0.925), but the concentration of TNF-α in group B was significantly higher than that in groups A and C (P<0.05). The ratio of CD4⁺T cell/CD8⁺T cell of venous blood had no significant difference between before and after operation in the same group (P=0.248), and between groups (P=0.646). Conclusion The freezing periosteum and bone marrow are lowly immunogenic. In order to decrease the immunogenicity of the joint, preserving the periosteum and removing the marrow cavity are recommended.

Citation： DENGJiuzheng, ZHANGYoule, LIXiang. IMMUNOGENICITY OF ALLOGENEIC FREEZING PERIOSTEUM AND BONE MARROW. Chinese Journal of Reparative and Reconstructive Surgery, 2014, 28(5): 649-653. doi: 10.7507/1002-1892.20140143 Copy

1.	Lexer E. Free transplantation. Ann Surg, 1914, 60(2):166-194.
2.	Hofmann GO, Kirschner MH, Wagner FD, et al. Allogeneic vascularized transplantation of human femoral diaphyses and total knee joints-first clinical experiences. Transplant Proc, 1998, 30(6):2754-2761.
3.	孫和炎, 卜海富, 王明麗, 等. 軟骨細胞培養及其凍存復蘇后的生物學特性比較. 安徽醫科大學學報, 2003, 38(2):107-109.
4.	文雪平, 張朝躍. 同種異體半關節移植治療下肢關節端骨腫瘤. 現代生物醫學進展, 2010, 10(13):2476-2478.
5.	Allieu Y, Marck G, Chammas M, et al. Total elbow joint allograft for long term posttraumatic osteoarticular loss. Follow-up results at twelve years. Rev Chir Orthop Reparatrice Appar Mot, 2004, 90(4):319-328.
6.	劉希云, 周從豪, 婁鐵塵, 等. 冷凍異體骨膜引導骨組織再生的實驗研究. 中華口腔醫學雜志, 2000, 35(5):385-387, I026.
7.	許永濤, 顧潔夫, 尚平. 深低溫保存的同種異體骨膜與胎骨聯合移植的實驗研究. 中國修復重建外科雜志, 2001, 15(3):183-187.
8.	張勇. 超深低溫條件下較長冷凍時間對異體神經移植免疫排斥的影響. 瀘州:瀘州醫學院, 2012.
9.	Minami A, An KN, Cooney WP 3rd, et al. Ligament stability of the metacarpophalangeal joint:a biomechanical study. J Hand Surg (Am), 1985, 10(2):255-260.
10.	鄭振輝, 周淑佩, 彭雙清. 實用醫學實驗動物學. 北京:北京大學醫學出版社, 2008:161-165.
11.	范學敏, 李鵬翠, 丁娟, 等. 玻璃化凍存對兔關節軟骨細胞存活率及生物學活性的影響. 中國骨科臨床與基礎研究雜志, 2012, 4(4):275-280.
12.	韓云, 劉軍, 藍妮, 等. 大鼠深低溫凍儲同種異體氣管移植的實驗研究. 中國醫科大學學報, 2011, 40(12):1079-1080, 1084.
13.	Lechler RI, Garden OA, Turka LA. The complementary roles of deletion and regulation in transplantation tolerance. Nat Rev Immunol, 2003, 3(2):147-158.
14.	朱志剛, 曹建民. 氬氦刀冷凍消融術對荷瘤兔T細胞免疫的近期影響. 介入放射學雜志, 2010, 19(7):558-559.
15.	沈勇, 孔榮, 朱六龍. 兔組織工程化骨異體植入修復骨缺損中外周血T淋巴細胞亞群的檢測. 安徽醫科大學學報, 2006, 41(4):392-395.
16.	宋紅星, 曹峻嶺, 劉淼, 等. 同種異體軟骨細胞移植修復兔膝關節軟骨缺損的免疫反應. 中國矯形外科雜志, 2002, 9(1):32-34.
17.	張羅生, 羅榮城, 鄭航, 等. RFA治療對VX2肝腫瘤兔免疫功能影響的實驗研究. 臨床腫瘤學雜志, 2002, 7(6):401-404.
18.	Zhao L, Zhao J, Wang S, et al. Evaluation of immunocompatibility of tissue-engineered periosteum. Biomed Meter, 2011, 6(1):015005.
19.	Czitrom AA, Axelrod T, Fernandes B. Antigen presenting cells and bone allotransplantation. Clin Orthop Relat Res, 1985, (197):27-31.
20.	馬以勇, 亓建洪. 冷凍保存對同種異體移植物免疫原性的影響. 泰山醫學院學報, 2007, 28(5):397-400.
21.	劉斌. 組織學與胚胎學. 北京:北京大學醫學出版社, 2003:67-68.
22.	Gotfried Y, Yaremchuk MJ, Randolph MA, et al. Histological characteristics of acute rejection in vascularized allografts of bone. J Bone Joint Surg (Am), 1987, 69(3):410-425.
23.	魏玲玲, 鄧紹平, 黃孝倫. 肝細胞移植治療急性肝功能衰竭的免疫排斥研究. 中國普外基礎與臨床雜志, 2012, 19(2):135-139.
24.	謝迎賓, 王強, 雷寧玉, 等. 兔角膜移植后房水中血管內皮生長因子及腫瘤壞死因子α表達與高壓氧的影響. 中國組織工程研究, 2012, 16(31):5763-5767.
25.	楊濤, 楊勇, 劉斌, 等. 兔異體脂肪干細胞移植的實驗研究. 現代生物醫學進展, 2013, 13(7):1244-1248.

1. Lexer E. Free transplantation. Ann Surg, 1914, 60(2):166-194.
2. Hofmann GO, Kirschner MH, Wagner FD, et al. Allogeneic vascularized transplantation of human femoral diaphyses and total knee joints-first clinical experiences. Transplant Proc, 1998, 30(6):2754-2761.
3. 孫和炎, 卜海富, 王明麗, 等. 軟骨細胞培養及其凍存復蘇后的生物學特性比較. 安徽醫科大學學報, 2003, 38(2):107-109.
4. 文雪平, 張朝躍. 同種異體半關節移植治療下肢關節端骨腫瘤. 現代生物醫學進展, 2010, 10(13):2476-2478.
5. Allieu Y, Marck G, Chammas M, et al. Total elbow joint allograft for long term posttraumatic osteoarticular loss. Follow-up results at twelve years. Rev Chir Orthop Reparatrice Appar Mot, 2004, 90(4):319-328.
6. 劉希云, 周從豪, 婁鐵塵, 等. 冷凍異體骨膜引導骨組織再生的實驗研究. 中華口腔醫學雜志, 2000, 35(5):385-387, I026.
7. 許永濤, 顧潔夫, 尚平. 深低溫保存的同種異體骨膜與胎骨聯合移植的實驗研究. 中國修復重建外科雜志, 2001, 15(3):183-187.
8. 張勇. 超深低溫條件下較長冷凍時間對異體神經移植免疫排斥的影響. 瀘州:瀘州醫學院, 2012.
9. Minami A, An KN, Cooney WP 3rd, et al. Ligament stability of the metacarpophalangeal joint:a biomechanical study. J Hand Surg (Am), 1985, 10(2):255-260.
10. 鄭振輝, 周淑佩, 彭雙清. 實用醫學實驗動物學. 北京:北京大學醫學出版社, 2008:161-165.
11. 范學敏, 李鵬翠, 丁娟, 等. 玻璃化凍存對兔關節軟骨細胞存活率及生物學活性的影響. 中國骨科臨床與基礎研究雜志, 2012, 4(4):275-280.
12. 韓云, 劉軍, 藍妮, 等. 大鼠深低溫凍儲同種異體氣管移植的實驗研究. 中國醫科大學學報, 2011, 40(12):1079-1080, 1084.
13. Lechler RI, Garden OA, Turka LA. The complementary roles of deletion and regulation in transplantation tolerance. Nat Rev Immunol, 2003, 3(2):147-158.
14. 朱志剛, 曹建民. 氬氦刀冷凍消融術對荷瘤兔T細胞免疫的近期影響. 介入放射學雜志, 2010, 19(7):558-559.
15. 沈勇, 孔榮, 朱六龍. 兔組織工程化骨異體植入修復骨缺損中外周血T淋巴細胞亞群的檢測. 安徽醫科大學學報, 2006, 41(4):392-395.
16. 宋紅星, 曹峻嶺, 劉淼, 等. 同種異體軟骨細胞移植修復兔膝關節軟骨缺損的免疫反應. 中國矯形外科雜志, 2002, 9(1):32-34.
17. 張羅生, 羅榮城, 鄭航, 等. RFA治療對VX2肝腫瘤兔免疫功能影響的實驗研究. 臨床腫瘤學雜志, 2002, 7(6):401-404.
18. Zhao L, Zhao J, Wang S, et al. Evaluation of immunocompatibility of tissue-engineered periosteum. Biomed Meter, 2011, 6(1):015005.
19. Czitrom AA, Axelrod T, Fernandes B. Antigen presenting cells and bone allotransplantation. Clin Orthop Relat Res, 1985, (197):27-31.
20. 馬以勇, 亓建洪. 冷凍保存對同種異體移植物免疫原性的影響. 泰山醫學院學報, 2007, 28(5):397-400.
21. 劉斌. 組織學與胚胎學. 北京:北京大學醫學出版社, 2003:67-68.
22. Gotfried Y, Yaremchuk MJ, Randolph MA, et al. Histological characteristics of acute rejection in vascularized allografts of bone. J Bone Joint Surg (Am), 1987, 69(3):410-425.
23. 魏玲玲, 鄧紹平, 黃孝倫. 肝細胞移植治療急性肝功能衰竭的免疫排斥研究. 中國普外基礎與臨床雜志, 2012, 19(2):135-139.
24. 謝迎賓, 王強, 雷寧玉, 等. 兔角膜移植后房水中血管內皮生長因子及腫瘤壞死因子α表達與高壓氧的影響. 中國組織工程研究, 2012, 16(31):5763-5767.
25. 楊濤, 楊勇, 劉斌, 等. 兔異體脂肪干細胞移植的實驗研究. 現代生物醫學進展, 2013, 13(7):1244-1248.

Chinese Journal of Reparative and Reconstructive Surgery

IMMUNOGENICITY OF ALLOGENEIC FREEZING PERIOSTEUM AND BONE MARROW

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