Repair of segmental bone defects in rabbits’ radius with domestic porous tantalum encapsulated with pedicled fascial flap_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

WANG Hui ¹ , WANG Qian ² , ZHANG Hui ³ , SHI Wei ⁴ , LAI Zhenquan ⁵ , CUI Yishuang ⁵ , LI Qijia ⁵ ,  WANG Zhiqiang ⁴

1. Department of Hand Surgery, Tangshan Orthopaedic Hospital Affiliated to North China University of Science and Technology, Tangshan Hebei, 063000, P.R.China;
2. Department of Anatomy, Basic Medical College, North China University of Science and Technology, Tangshan Hebei, 063000, P.R.China;
3. Department of Joint Surgery, Tangshan Orthopaedic Hospital Affiliated to North China University of Science and Technology, Tangshan Hebei, 063000, P.R.China;
4. Department of Orthopaedics, Affiliated Hospital of North China University of Science and Technology, Tangshan Hebei, 063000, P.R.China;
5. Medical Experimental Research Center, North China University of Science and Technology, Tangshan Hebei, 063000, P.R.China;

Corresponding?author：

WANG Zhiqiang, Email: wzhqde@163.com

Keywords：

Porous tantalum; fascial fIap; segmental bone defect; bone repairing; Micro-CT; biomechanics

DOI：

10.7507/1002-1892.201611048

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Objective To investigate the effect of domestic porous tantalum encapsulated with pedicled fascial flap on repairing of segmental bone defect in rabbits’ radius. Methods A total of 60 New Zealand white rabbits (aged 6- 8 months and weighing 2.5-3.0 kg) were randomly divided into the experimental group and control group (30 rabbits each group). A 1.5 cm segmental bone defect in right radius was established as the animal model. The porous tantalums encapsulated with pedicled fascial flaps (30 mm×20 mm) were implanted in the created bone defect in the experimental group, and the porous tantalums were only implanted in the control group. X-ray films were observed at the day after operation and at 4, 8, and 16 weeks after operation. Specimens were taken out at 4, 8, and 16 weeks after operation for HE staining and toluidine blue staining observation. The maximum load force and bending strength were detected by three point bending biomechanical test, and the Micro-CT analysis and quantitative analysis of the new bone volume fraction (BV/TV) were performed at 16 weeks after operation to compare the bone defect repair abilityin vivo in 2 groups. Results All incisions healed by first intention without wound infection. At 4, 8, and 16 weeks after operation, the X-ray films showed that the implants were well maintained without apparent displacement. As followed with time, the combination between the implants and host bone became more and more closely, and the fracture line gradually disappeared. HE staining and toluidine blue staining showed that new bone mass and maturity gradually increased at the interface and inside materials in 2 groups, and the new bone gradually growed from the interface to internal pore. At 16 weeks after operation, the three point bending biomechanical test showed that the maximum load force and bending strength in the experimental were (96.54±7.21) N and (91.26±1.76) MPa respectively, showing significant differences when compared with the control group [(82.65±5.65) N and (78.53±1.16) MPa respectively] (t=3.715, P=0.004; t=14.801, P=0.000). And Micro-CT analysis exhibited that there were a large amount of new bone at the interface and the surface of implant materials and inside the materials. The new bone BV/TV in the experimental group (32.63%±3.56%) was significantly higher than that in control group (25.07%±4.34%) (t=3.299, P=0.008). Conclusion Domestic porous tantalum encapsulated with pedicled fascial flap can increase local blood supply, strengthen material bone conduction ability, and promote the segmental bone defect repair.

Citation： WANG Hui, WANG Qian, ZHANG Hui, SHI Wei, LAI Zhenquan, CUI Yishuang, LI Qijia, WANG Zhiqiang. Repair of segmental bone defects in rabbits’ radius with domestic porous tantalum encapsulated with pedicled fascial flap. Chinese Journal of Reparative and Reconstructive Surgery, 2017, 31(10): 1200-1207. doi: 10.7507/1002-1892.201611048 Copy

1.	Marin E, Fedrizzi L, Zagra L. Porous metallic structures for orthopaedic applications: a short review of materials and technologies. Eur Orthop Traumatol, 2010, 1(3-4): 103-109.
2.	Paganias CG, Tsakotos GA, Koutsostathis SD, et al. Osseous integration in porous tantalum implants. Indian J Orthop, 2012, 46(5): 505-513.
3.	Wang Q, Zhang H, Li Q, et al. Biocompatibility and osteogenic properties of porous tantalum. Exp Ther Med, 2015, 9(3): 780-786.
4.	Wigfield C, Robertson J, Gill S, et al. Clinical experience with porous tantalum cervical interbody implants in a prospective randomized controlled trial. Br J Neurosurg, 2003, 17(5): 418-425.
5.	Kamath AF, Lewallen DG, Hanssen AD. Porous tantalum metaphyseal cones for severe tibial bone loss in revision knee arthroplasty: a five to nine-year follow-up. J Bone Joint Surg (Am), 2015, 97(3): 216-223.
6.	Ren B, Zhai Z, Guo K, et al. The application of porous tantalum cylinder to the repair of comminuted bone defects: a study of rabbit firearm injuries. Int J Clin Exp Med, 2015, 8(4): 5055-5064.
7.	王茜, 張輝, 耿麗鑫, 等. 國產多孔鉭復合 MG63 細胞培養 Col-1、OC 和 OPN 蛋白表達及意義. 中國矯形外科雜志, 2015, 23(5): 441-449.0.
8.	李琪佳, 王茜, 甘洪全, 等. 多孔鉭材料細胞毒性、生物相容性及體內成骨性研究. 中華骨科雜志, 2014, 34(9): 954-961.0.
9.	耿麗鑫, 甘洪全, 王茜, 等. 國產多孔鉭對成骨細胞生物相容性及其相關成骨基因表達的影響. 第三軍醫大學學報, 2014, 36(11): 1163-1167.0.
10.	王茜, 張輝, 耿麗鑫, 等. MG63 細胞與國產多孔鉭材料共培養后成骨相關因子的表達研究. 中國修復重建外科雜志, 2014, 28(11): 1422-1427.0.
11.	趙御森, 楊新明, 王海波. 比較帶蒂筋膜瓣與生物膜包繞組織工程骨修復兔超臨界骨缺損的實驗研究. 安徽醫科大學學報, 2013, 48(6): 621-625.0.
12.	楊新明, 孟憲勇, 王耀一, 等. 帶蒂筋膜瓣包裹自體紅骨髓組織工程復合體修復四肢大段骨缺損臨床研究. 中國醫師進修雜志, 2011, 34(23): 1-4.0.
13.	Periasamy K, Watson WS, Mohammed A, et al. A randomised study of peri-prosthetic bone density after cemented versus trabecular fixation of a polyethylene acetabular component. J Bone Joint Surg (Br), 2011, 93(8): 1033-1044.
14.	Sinclair SK, Konz GJ, Dawson JM, et al. Host bone response to polyetheretherketone versus porous tantalum implants for cervical spinal fusion in a goat model. Spine (Phila Pa 1976), 2012, 37(10): E571-580.
15.	Bai F, Wang Z, Lu J, et al. The correlation between the internal structure and vascularization of controllable porous bioceramic materials in vivo: a quantitative study. Tissue Eng Part A, 2010, 16(12): 3791-3803.
16.	丁冉, 吳志宏, 邱貴興, 等. 選擇性激光燒結技術的多孔鈦合金支架的骨組織工程學觀察. 中華醫學雜志, 2014, 94(19): 1499-1502.0.
17.	Li X, Lin Z, Duan Y, et al. Repair of large segmental bone defects in rabbits using BMP and FGF composite xenogeneic bone. Genet Mol Res, 2015, 14(2): 6395-6400.
18.	Kroese-Deutman HC, Vehof JW, Spauwen PH, et al. Orthotopic bone formation in titanium fiber mesh loaded with platelet-rich plasma and placed in segmental defects. Int J Oral Maxillofac Surg, 2008, 37(6): 542-549.
19.	Roohani-Esfahani SI, Dunstan CR, Davies B, et al. Repairing a critical-sized bone defect with highly porous modified and unmodified baghdadite scaffolds. Acta Biomater, 2012, 8(11): 4162-4172.
20.	Kim J, McBride S, Donovan A, et al. Tyrosine-derived polycarbonate scaffolds for bone regeneration in a rabbit radius critical-size defect model. Biomed Mater, 2015, 10(3): 035001.
21.	劉勇, 裴國獻, 江汕, 等. 帶蒂筋膜瓣促組織工程骨修復兔大段骨缺損的動態研究.中華實驗外科雜志, 2007, 24(6): 752.0.
22.	楊新明, 石蔚, 杜雅坤, 等. 以帶蒂筋膜瓣包裹復合自體紅骨髓構建非細胞型組織工程化骨修復兔橈骨缺損. 中國組織工程研究與臨床, 2009, 13(46): 9050-9054.0.
23.	王海波, 楊新明, 趙御森. 帶蒂筋膜瓣的穩定性對其促超臨界骨缺損成骨作用影響的實驗研究. 安徽醫科大學學報, 2013, 48(9): 1044-1048.0.

1. Marin E, Fedrizzi L, Zagra L. Porous metallic structures for orthopaedic applications: a short review of materials and technologies. Eur Orthop Traumatol, 2010, 1(3-4): 103-109.
2. Paganias CG, Tsakotos GA, Koutsostathis SD, et al. Osseous integration in porous tantalum implants. Indian J Orthop, 2012, 46(5): 505-513.
3. Wang Q, Zhang H, Li Q, et al. Biocompatibility and osteogenic properties of porous tantalum. Exp Ther Med, 2015, 9(3): 780-786.
4. Wigfield C, Robertson J, Gill S, et al. Clinical experience with porous tantalum cervical interbody implants in a prospective randomized controlled trial. Br J Neurosurg, 2003, 17(5): 418-425.
5. Kamath AF, Lewallen DG, Hanssen AD. Porous tantalum metaphyseal cones for severe tibial bone loss in revision knee arthroplasty: a five to nine-year follow-up. J Bone Joint Surg (Am), 2015, 97(3): 216-223.
6. Ren B, Zhai Z, Guo K, et al. The application of porous tantalum cylinder to the repair of comminuted bone defects: a study of rabbit firearm injuries. Int J Clin Exp Med, 2015, 8(4): 5055-5064.
7. 王茜, 張輝, 耿麗鑫, 等. 國產多孔鉭復合 MG63 細胞培養 Col-1、OC 和 OPN 蛋白表達及意義. 中國矯形外科雜志, 2015, 23(5): 441-449.0.
8. 李琪佳, 王茜, 甘洪全, 等. 多孔鉭材料細胞毒性、生物相容性及體內成骨性研究. 中華骨科雜志, 2014, 34(9): 954-961.0.
9. 耿麗鑫, 甘洪全, 王茜, 等. 國產多孔鉭對成骨細胞生物相容性及其相關成骨基因表達的影響. 第三軍醫大學學報, 2014, 36(11): 1163-1167.0.
10. 王茜, 張輝, 耿麗鑫, 等. MG63 細胞與國產多孔鉭材料共培養后成骨相關因子的表達研究. 中國修復重建外科雜志, 2014, 28(11): 1422-1427.0.
11. 趙御森, 楊新明, 王海波. 比較帶蒂筋膜瓣與生物膜包繞組織工程骨修復兔超臨界骨缺損的實驗研究. 安徽醫科大學學報, 2013, 48(6): 621-625.0.
12. 楊新明, 孟憲勇, 王耀一, 等. 帶蒂筋膜瓣包裹自體紅骨髓組織工程復合體修復四肢大段骨缺損臨床研究. 中國醫師進修雜志, 2011, 34(23): 1-4.0.
13. Periasamy K, Watson WS, Mohammed A, et al. A randomised study of peri-prosthetic bone density after cemented versus trabecular fixation of a polyethylene acetabular component. J Bone Joint Surg (Br), 2011, 93(8): 1033-1044.
14. Sinclair SK, Konz GJ, Dawson JM, et al. Host bone response to polyetheretherketone versus porous tantalum implants for cervical spinal fusion in a goat model. Spine (Phila Pa 1976), 2012, 37(10): E571-580.
15. Bai F, Wang Z, Lu J, et al. The correlation between the internal structure and vascularization of controllable porous bioceramic materials in vivo: a quantitative study. Tissue Eng Part A, 2010, 16(12): 3791-3803.
16. 丁冉, 吳志宏, 邱貴興, 等. 選擇性激光燒結技術的多孔鈦合金支架的骨組織工程學觀察. 中華醫學雜志, 2014, 94(19): 1499-1502.0.
17. Li X, Lin Z, Duan Y, et al. Repair of large segmental bone defects in rabbits using BMP and FGF composite xenogeneic bone. Genet Mol Res, 2015, 14(2): 6395-6400.
18. Kroese-Deutman HC, Vehof JW, Spauwen PH, et al. Orthotopic bone formation in titanium fiber mesh loaded with platelet-rich plasma and placed in segmental defects. Int J Oral Maxillofac Surg, 2008, 37(6): 542-549.
19. Roohani-Esfahani SI, Dunstan CR, Davies B, et al. Repairing a critical-sized bone defect with highly porous modified and unmodified baghdadite scaffolds. Acta Biomater, 2012, 8(11): 4162-4172.
20. Kim J, McBride S, Donovan A, et al. Tyrosine-derived polycarbonate scaffolds for bone regeneration in a rabbit radius critical-size defect model. Biomed Mater, 2015, 10(3): 035001.
21. 劉勇, 裴國獻, 江汕, 等. 帶蒂筋膜瓣促組織工程骨修復兔大段骨缺損的動態研究.中華實驗外科雜志, 2007, 24(6): 752.0.
22. 楊新明, 石蔚, 杜雅坤, 等. 以帶蒂筋膜瓣包裹復合自體紅骨髓構建非細胞型組織工程化骨修復兔橈骨缺損. 中國組織工程研究與臨床, 2009, 13(46): 9050-9054.0.
23. 王海波, 楊新明, 趙御森. 帶蒂筋膜瓣的穩定性對其促超臨界骨缺損成骨作用影響的實驗研究. 安徽醫科大學學報, 2013, 48(9): 1044-1048.0.

Chinese Journal of Reparative and Reconstructive Surgery

Repair of segmental bone defects in rabbits’ radius with domestic porous tantalum encapsulated with pedicled fascial flap

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