Expressions and clinical significance of SDF-1 and CXCR4 in local tissues of perianal abscess_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 HAO Zhi ¹ , ZHEN Hongbo ² , GONG Haifeng ¹

1. Department of General Surgery, Beijing First Hospital of Integrated Chinese and Western Medicine , Beijing 100026, P. R. China;
2. Department of Eye, Beijing Sixth Hospital, Beijing 100007, P. R. China;

Corresponding?author：

HAO Zhi, Email: long.2659@163.com

Keywords：

perianal abscess; stromal cell derived factor 1; chemokine receptor-4; clinicopathological feature; recurrence

DOI：

10.7507/1007-9424.201906085

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the expressions of stromal cell-derived factor-1 (SDF-1) and chemokine receptor-4 (CXCR4) in local tissues of perianal abscess and their relationships with clinicopathological features and prognosis of patients.Methods A total of 47 patients with perianal abscess (perianal abscess group) and 58 patients with mixed hemorrhoids (mixed hemorrhoids group) were selected for the study. The tissues were collected during the operation. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expressions of SDF-1 mRNA and CXCR4 mRNA in local tissues of the two groups, the positive expressions of SDF-1 protein and CXCR4 protein in local tissues were detected by immunohistochemistry, and the relationships between the expressions of SDF-1 and CXCR4 protein and the clinical characteristics, prognosis of patients were analyzed.Results The expression levels of SDF-1 mRNA and CXCR4 mRNA in the perianal abscess group were higher than those in the mixed hemorrhoids group, and the positive rates of SDF-1 protein and CXCR4 protein in the perianal abscess group were higher than those in the mixed hemorrhoids group too (P<0.05). The expressions of SDF-1 protein and CXCR4 protein in perianal abscess tissues were both not related to sex, age, location of abscess, and course of disease (P>0.05), but was related to abscess diameter, healing time, and anal fistula (P<0.05). The non-recurrence rates of SDF-1 protein-negative group and CXCR4 protein-negative group were lower than those of SDF-1 protein-positive group and CXCR4 protein-positive group respectively (P<0.05).Conclusion SDF-1 and CXCR4 molecular are up-regulated in the local tissues of perianal abscess, which are related to the size of abscess, healing time, anal fistula, and recurrence of patients.

Citation： HAO Zhi, ZHEN Hongbo, GONG Haifeng. Expressions and clinical significance of SDF-1 and CXCR4 in local tissues of perianal abscess. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2019, 26(12): 1434-1439. doi: 10.7507/1007-9424.201906085 Copy

1.	陳永樂, 王世霞. 1 330 例肛腸疾病患者發病特征分析. 臨床醫學研究與實踐, 2018, 3(20): 119-120.
2.	焦峰, 仲艷陽, 何秀芝, 等. 高位肛周膿腫切開術后不同引流方式的臨床對照研究. 實用醫學雜志, 2018, 34(13): 2275-2276, 2279.
3.	Zheng F, Zhang Z, Flamini V, et al. The axis of CXCR4/SDF-1 plays a role in colon cancer cell adhesion through regulation of the AKT and IGF1R signalling pathways. Anticancer Res, 2017, 37(8): 4361-4369.
4.	林旭紅, 王丹丹, 王慧超, 等. CXCR4 在潰瘍性結腸炎小鼠蛋白 C 系統變化中的作用. 中國病理生理雜志, 2016, 32(10): 1854-1862.
5.	劉瑞, 羅前程, 朱鴻玲, 等. IL-4 和 IL-13 在甲狀腺乳頭狀癌中的表達及其與臨床病理特征和預后的關系. 臨床與病理雜志, 2018, 38(3): 490-497.
6.	郁俊文, 劉旭元. 一期手術治療肛周膿腫 120 例. 中國肛腸病雜志, 2012, 32(12): 20.
7.	張旭標, 林濤, 曾永親. 膠質瘤中間質細胞衍生因子-1 及神經生長因子的表達及臨床意義. 河北醫藥, 2017, 39(15): 2250-2253.
8.	王建平, 杜金林, 蔡成, 等. 肛周膿腫局部組織及外周血中 IL-22 的表達及臨床意義. 浙江醫學, 2015, 37(11): 958-960, 963.
9.	曹影. 肛周膿腫檢測中外周血 C 反應蛋白、纖維蛋白原含量對其診治的臨床意義. 中國肛腸病雜志, 2019, 39(1): 13-14.
10.	陶勇, 楊關根, 裘建明, 等. 人催乳素受體蛋白在肛周膿腫患者中的表達及其與預后的關系. 中華結直腸疾病電子雜志, 2017, 6(6): 488-493.
11.	Huang X, Mao W, Zhang T, et al. Baicalin promotes apoptosis and inhibits proliferation and migration of hypoxia-induced pulmonary artery smooth muscle cells by up-regulating A2a receptor via the SDF-1/CXCR4 signaling pathway. BMC Complement Altern Med, 2018, 18(1): 330-337.
12.	Ando N, Furuichi Y, Kasai S, et al. Chemosensitivity is differentially regulated by the SDF-1/CXCR4 and SDF-1/CXCR7 axes in acute lymphoblastic leukemia with MLL gene rearrangements. Leuk Res, 2018, 75(12): 36-44.
13.	陳艷昕, 劉慶濱, 周穎, 等. Vasohibin-1、PD-1、SDF-1 在結直腸癌患者中的表達及其與臨床特征的關系. 癌癥進展, 2018, 16(5): 643-646.
14.	周琦, 張寧, 盧芳, 等. 穿山龍總皂苷對痛風性關節炎大鼠關節炎滑膜 IL-1β 及其信號轉導通路的影響. 中藥藥理與臨床, 2013, 29(6): 52-57.
15.	Margaritopoulos GA, Antoniou KM, Lasithiotaki I, et al. Expression of SDF-1/CXCR4 axis in bone marrow mesenchymal stem cells derived from rheumatoid arthritis-usual interstitial pneumonia. Clin Exp Rheumatol, 2013, 31(4): 610-611.
16.	宋爽, 李虹, 殷少軍, 等. 基質細胞衍生因子-1 在慢性阻塞性肺疾病患者誘導痰中的表達及臨床意義. 國際呼吸雜志, 2018, 38(21): 1619-1623.
17.	顧園, 龔攀. SDF-1/CXCR4 軸在潰瘍性結腸炎病理過程中的作用. 中國臨床研究, 2018, 31(6): 757-759.
18.	張磊, 孫雪竹, 孫景洲. SDF-1/CXCR4 及 VEGF 在結直腸腺癌中的表達及其意義. 中國免疫學雜志, 2015, 31(5): 674-677, 682.
19.	陳志京, 廖山嬰, 吳伍林, 等. 趨化因子 CCL20、SDF-1 在大腸癌中的表達及其與淋巴結及肝臟轉移的關系. 廣東醫學, 2018, 39(17): 2598-2601, 2605.
20.	Ramonell KM, Zhang W, Hadley A, et al. CXCR4 blockade decreases CD4⁺ T cell exhaustion and improves survival in a murine model of polymicrobial sepsis. PLoS One, 2017, 12(12): e0188882.
21.	Patry C, Stamm D, Betzen C, et al. CXCR-4 expression by circulating endothelial progenitor cells and SDF-1 serum levels are elevated in septic patients. J Inflamm (Lond), 2018, 15(1): 10-18.
22.	李偉, 邢國輝, 董佃國. 原發性肝癌 SDF-1/CXCR4 軸與血管生成和病灶轉移相關性研究. 中華腫瘤防治雜志, 2017, 24(9): 606-610.
23.	Baran M, ?a?an Appak Y, Garipcin P, et al. The role of familial mediterranean fever gene mutation in treatment of infantile colitis with resistant perianal fistula. Arch Rheumatol, 2018, 33(4): 473-477.
24.	Weixler B, Renetseder F, Facile I, et al. Phosphorylated CXCR4 expression has a positive prognostic impact in colorectal cancer. Cell Oncol (Dordr), 2017, 40(6): 609-619.
25.	Cotoia A, Mirabella L, Altamura S, et al. Circulating stem cells, HIF-1, and SDF-1 in septic abdominal surgical patients: randomized controlled study protocol. Trials, 2018, 19(1): 179-186.

1. 陳永樂, 王世霞. 1 330 例肛腸疾病患者發病特征分析. 臨床醫學研究與實踐, 2018, 3(20): 119-120.
2. 焦峰, 仲艷陽, 何秀芝, 等. 高位肛周膿腫切開術后不同引流方式的臨床對照研究. 實用醫學雜志, 2018, 34(13): 2275-2276, 2279.
3. Zheng F, Zhang Z, Flamini V, et al. The axis of CXCR4/SDF-1 plays a role in colon cancer cell adhesion through regulation of the AKT and IGF1R signalling pathways. Anticancer Res, 2017, 37(8): 4361-4369.
4. 林旭紅, 王丹丹, 王慧超, 等. CXCR4 在潰瘍性結腸炎小鼠蛋白 C 系統變化中的作用. 中國病理生理雜志, 2016, 32(10): 1854-1862.
5. 劉瑞, 羅前程, 朱鴻玲, 等. IL-4 和 IL-13 在甲狀腺乳頭狀癌中的表達及其與臨床病理特征和預后的關系. 臨床與病理雜志, 2018, 38(3): 490-497.
6. 郁俊文, 劉旭元. 一期手術治療肛周膿腫 120 例. 中國肛腸病雜志, 2012, 32(12): 20.
7. 張旭標, 林濤, 曾永親. 膠質瘤中間質細胞衍生因子-1 及神經生長因子的表達及臨床意義. 河北醫藥, 2017, 39(15): 2250-2253.
8. 王建平, 杜金林, 蔡成, 等. 肛周膿腫局部組織及外周血中 IL-22 的表達及臨床意義. 浙江醫學, 2015, 37(11): 958-960, 963.
9. 曹影. 肛周膿腫檢測中外周血 C 反應蛋白、纖維蛋白原含量對其診治的臨床意義. 中國肛腸病雜志, 2019, 39(1): 13-14.
10. 陶勇, 楊關根, 裘建明, 等. 人催乳素受體蛋白在肛周膿腫患者中的表達及其與預后的關系. 中華結直腸疾病電子雜志, 2017, 6(6): 488-493.
11. Huang X, Mao W, Zhang T, et al. Baicalin promotes apoptosis and inhibits proliferation and migration of hypoxia-induced pulmonary artery smooth muscle cells by up-regulating A2a receptor via the SDF-1/CXCR4 signaling pathway. BMC Complement Altern Med, 2018, 18(1): 330-337.
12. Ando N, Furuichi Y, Kasai S, et al. Chemosensitivity is differentially regulated by the SDF-1/CXCR4 and SDF-1/CXCR7 axes in acute lymphoblastic leukemia with MLL gene rearrangements. Leuk Res, 2018, 75(12): 36-44.
13. 陳艷昕, 劉慶濱, 周穎, 等. Vasohibin-1、PD-1、SDF-1 在結直腸癌患者中的表達及其與臨床特征的關系. 癌癥進展, 2018, 16(5): 643-646.
14. 周琦, 張寧, 盧芳, 等. 穿山龍總皂苷對痛風性關節炎大鼠關節炎滑膜 IL-1β 及其信號轉導通路的影響. 中藥藥理與臨床, 2013, 29(6): 52-57.
15. Margaritopoulos GA, Antoniou KM, Lasithiotaki I, et al. Expression of SDF-1/CXCR4 axis in bone marrow mesenchymal stem cells derived from rheumatoid arthritis-usual interstitial pneumonia. Clin Exp Rheumatol, 2013, 31(4): 610-611.
16. 宋爽, 李虹, 殷少軍, 等. 基質細胞衍生因子-1 在慢性阻塞性肺疾病患者誘導痰中的表達及臨床意義. 國際呼吸雜志, 2018, 38(21): 1619-1623.
17. 顧園, 龔攀. SDF-1/CXCR4 軸在潰瘍性結腸炎病理過程中的作用. 中國臨床研究, 2018, 31(6): 757-759.
18. 張磊, 孫雪竹, 孫景洲. SDF-1/CXCR4 及 VEGF 在結直腸腺癌中的表達及其意義. 中國免疫學雜志, 2015, 31(5): 674-677, 682.
19. 陳志京, 廖山嬰, 吳伍林, 等. 趨化因子 CCL20、SDF-1 在大腸癌中的表達及其與淋巴結及肝臟轉移的關系. 廣東醫學, 2018, 39(17): 2598-2601, 2605.
20. Ramonell KM, Zhang W, Hadley A, et al. CXCR4 blockade decreases CD4⁺ T cell exhaustion and improves survival in a murine model of polymicrobial sepsis. PLoS One, 2017, 12(12): e0188882.
21. Patry C, Stamm D, Betzen C, et al. CXCR-4 expression by circulating endothelial progenitor cells and SDF-1 serum levels are elevated in septic patients. J Inflamm (Lond), 2018, 15(1): 10-18.
22. 李偉, 邢國輝, 董佃國. 原發性肝癌 SDF-1/CXCR4 軸與血管生成和病灶轉移相關性研究. 中華腫瘤防治雜志, 2017, 24(9): 606-610.
23. Baran M, ?a?an Appak Y, Garipcin P, et al. The role of familial mediterranean fever gene mutation in treatment of infantile colitis with resistant perianal fistula. Arch Rheumatol, 2018, 33(4): 473-477.
24. Weixler B, Renetseder F, Facile I, et al. Phosphorylated CXCR4 expression has a positive prognostic impact in colorectal cancer. Cell Oncol (Dordr), 2017, 40(6): 609-619.
25. Cotoia A, Mirabella L, Altamura S, et al. Circulating stem cells, HIF-1, and SDF-1 in septic abdominal surgical patients: randomized controlled study protocol. Trials, 2018, 19(1): 179-186.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expressions and clinical significance of SDF-1 and CXCR4 in local tissues of perianal abscess

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content