Influence on the expression of SPARC and GLUT-4 protein in adipose tissue ofnon-obese GK rats after gastric bypass operation_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YAO Dan ¹ , ZHANG Pengbo ² , GONG Shuai ² , LIU Li ² , WANG Jike ² ,  REN Zeqiang ²

1. Department of Gastrointestinal Surgery, The Second People’s Hospital of Huai’an, Huai’an, Jiangsu 223000, P. R. China;
2. Department of Pancreatic Surgery, The Affiliated Hospital of Xuzhou Meidcal University, Xuzhou, Jiangsu 221000, P. R. China;

Corresponding?author：

REN Zeqiang, Email: rzq0805@189.cn

Keywords：

gastric bypass operation; secreted protein acidic and rich in cysteine; glucose transporter-4; insulin resistance

DOI：

10.7507/1007-9424.201908091

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Objective To investigate the expressions of glucose transporter-4 (GLUT-4) and secreted protein acidic and rich in cysteine (SPARC) in adipose tissue of Goto-Kakizaki (GK)/Wister rats after gastric bypass operation (GBP), and to explore the possible mechanism of GBP improving insulin resistance.Methods Healthy male GK rats were randomly divided into diabetic operation group (DO group, underwent GBP), diabetic sham operation group (DS group, underwent sham-operation), and diabetic control group (DC group, received no-treatment), Wister rats were set as normal control group (NC group, received no-treatment). The weight, fasting blood glucose (FPG), fasting serum insulin (Fins), and HbA1c were measured before operation and at the 1st, 2nd, 4th, and 8th week after operation. Quantitative insulin sensitivity check index (QUICKI) was measured in before operation and at the 8th week after operation, and the expressions of GLUT-4 and SPARC protein in adipose tissues were measured by Western Blot method at the 8th week after operative too.Results ① Weight: the weight of the DO group was lower than preoperative at the 2nd and 4th week after GBP (P<0.05), but increased to the normal level at the 8th week after GBP (P>0.05). The weight of the DO group was lower than those of the DS group, DC group, and NC group at the same time point of 2nd, 4th, and 8th week (P<0.05). ② FPG: the FPG level of the DO group was lower than preoperative at the 2nd, 4th, and 8th week after GBP (P<0.05), and lower than those of the DS group and the DC group from 2nd to 8th week after GBP (P<0.05). The FPG level between the DS group and the DC group had no statistical significance (P>0.05). ③ Fins: the Fins level of the DO group was higher than preoperative at the 2nd week after GBP (P<0.05), and decreased gradually at 4th and 8th week but not significantly differed from the NC group at the same time point. At the 2nd week after GBP, the Fins level of the DO group was higher than those of the DS group and the DC group (P<0.05), but there was no statistical significance between the DS group and the DC group (P>0.05). ④ HbA1c: the HbA1c level of the DO group started to decrease but there was no statistical significance between preoperative and all time after GBP (P>0.05). There was no statistical significance among the 4 groups at the 8th week after GBP (P>0.05). ⑤ QUICKI: at the 8th week, the QUICKI value of the DO group was higher than preoperative (P<0.05), and at the same time, the QUICKI value of the DO group and the NC group were higher than those of the DS group and the DC group (P<0.05), but there was no statistical significance between the DO group and the NC group (P>0.05), as well as the DS group and the DC group (P>0.05). ⑥ GLUT-4 protein and SPARC protein: the expression of GLUT-4 protein of the DO group was dramatically higher than those of the DC, DS, and NC group (P<0.05), and the expression of SPARC protein of the DO group was dramatically lower than those of the DC, DS, and NC group (P<0.05) at the 8th week. But the expressions of GLUT-4 and SPARC protein had no statistical significance among the DS, DC, and NC group at the 8th week after GBP (P>0.05).Conclusion GBP may improve and increase the sensitivity of insulin resistance by regulating the expressions of GLUT-4 and SPARC protein in adipose tissue of GK rats.

Citation： YAO Dan, ZHANG Pengbo, GONG Shuai, LIU Li, WANG Jike, REN Zeqiang. Influence on the expression of SPARC and GLUT-4 protein in adipose tissue ofnon-obese GK rats after gastric bypass operation. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2020, 27(6): 685-690. doi: 10.7507/1007-9424.201908091 Copy

1.	Pories WJ, Swanson MS, MacDonald KG, et al. Who would have thought it? An operation proves to be the most effective therapy for adult-onset diabetes mellitus. Ann Surg, 1995, 222(3): 339-350.
2.	溫雨晴, 譚迎春, 張蓬波, 等. 胃轉流術對 2 型糖尿病大鼠脂肪組織胰島素抵抗的影響. 中華實驗外科雜志, 2013, 30(11): 2324-2326.
3.	張蓬波, 陳守坤, 張秀忠, 等. 胃轉流術對 2 型糖尿病大鼠肝組織胰島素受體底物-2、葡萄糖轉運體-2 表達的影響. 中華實驗外科雜志, 2013, 30(12): 2587-2589.
4.	張秀忠, 任澤強, 張蓬波. 胃轉流術對 2 型糖尿病大鼠的降糖作用及對糖耐量和胰島素抵抗的影響. 中華實驗外科雜志, 2010, 27(12): 1892-1894.
5.	龔帥, 任澤強, 張蓬波, 等. 胃轉流術對非肥胖型 2 型糖尿病大鼠骨骼肌胰島素受體底物-1 及泛素化蛋白表達的影響. 中國普外基礎與臨床雜志, 2016, 23(8): 915-921.
6.	Shao J, Yamashita H, Qiao L, et al. Decreased Akt kinase activity and insulin resistance in C57BL/KsJ-Leprdb/db mice. J Endocrinol, 2000, 167(1): 107-115.
7.	譚柏宏, 張立海, 王躍生, 等. 胃轉流術對 2 型糖尿病大鼠棕色脂肪組織的影響. 黑龍江醫藥科學, 2018, 41(1): 39-40, 34.
8.	Yu H, Li Q, Zhang M, et al. Decreased leptin is associated with alterations in thyroid-stimulating hormone levels after Roux-en-Y gastric bypass surgery in obese euthyroid patients with type 2 diabetes. Obes Facts, 2019, 12(3): 272-280.
9.	李晗, 高宏凱, 張新國, 等. 胃轉流手術后 GK 大鼠血糖變化與攝食量及體質量的關系. 中華實驗外科雜志, 2010, 27(8): 1069-1070.
10.	張強, 陳軒, 徐亮. 胃轉流術治療 2 型糖尿病與 JNK 信號通路的關系. 中國普外基礎與臨床雜志, 2018, 25(9): 1055-1059.
11.	Xu J, Yang S, Gu X, et al. SPARC correlates with unfavorable outcome and promotes tumor growth in lung squamous cell carcinoma. Exp Mol Pathol, 2019, 110: 104276.
12.	黃海波, 趙燕, 楊樹軍. 富含半胱氨酸的酸性分泌蛋白在食管癌中的研究進展. 國際腫瘤學雜志, 2014, 41(12): 918-921.
13.	申玉翠, 張其勝, 姚憶蓉, 等. 結腸癌組織 SPARC 蛋白質的表達及其臨床意義. 實用醫學雜志, 2014, 30(20): 3277-3279.
14.	孫烈, 陳國衛, 尹杰, 等. 富含半胱氨酸的酸性分泌蛋白對胰腺癌細胞增殖、遷移和侵襲能力的影響. 中華實驗外科雜志, 2014, 31(9): 1954-1956.
15.	Jiang X, Liu F, Wang Y, et al. Secreted protein acidic and rich in cysteine promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells and acquisition of cancer stem cell phenotypes. J Gastroenterol Hepatol, 2019, 34(10): 1860-1868.
16.	Frangogiannis NG. Matricellular proteins in cardiac adaptation and disease. Physiol Rev, 2012, 92(2): 635-688.
17.	羅麗穎, 吳佳慧, 項瀟瓊, 等. 富含半胱氨酸的酸性分泌蛋白對青光眼濾過泡瘢痕化影響的研究進展. 上海交通大學學報: 醫學版, 2019, 39(6): 684-688.
18.	劉鍇, 宋海燕, 劉姝, 等. 2 型糖尿病肥胖患者 SPARC 表達差異性研究. 中華內分泌代謝雜志, 2014, 30(5): 388-392.
19.	Takahashi M, Nagaretani H, Funahashi T, et al. The expression of SPARC in adipose tissue and its increased plasma concentration in patients with coronary artery disease. Obes Res, 2001, 9(7): 388-393.
20.	Aoi W, Hirano N, Lassiter DG, et al. Secreted protein acidic and rich in cysteine (SPARC) improves glucose tolerance. FASEB J, 2019, 33(9): 10551-10562.
21.	Tartare-Deckert S, Chavey C, Monthouel MN, et al. The matri-cellular protein SPARC/osteonectin as a newly identified factor up-regulated in obesity. J Biol Chem, 2001, 276(25): 22231-22237.
22.	Nie J, Sage EH. SPARC inhibits adipogenesis by its enhancement of beta-catenin signaling. J Biol Chem, 2009, 284(2): 1279-1290.
23.	Harries LW, McCulloch LJ, Holley JE, et al. A role for SPARC in the moderation of human insulin secretion. PLoS One, 2013, 8(6): e68253.
24.	Song H, Guan Y, Zhang L, et al. SPARC interacts with AMPK and regulates GLUT4 expression. Biochem Biophys Res Commun, 2010, 396(4): 961-966.
25.	Jing Y, Jin Y, Wang Y, et al. SPARC promotes the proliferation and metastasis of oral squamous cell carcinoma by PI3K/AKT/PDGFB/PDGFRβ axis. J Cell Physiol, 2019, [Epub ahead of print].

1. Pories WJ, Swanson MS, MacDonald KG, et al. Who would have thought it? An operation proves to be the most effective therapy for adult-onset diabetes mellitus. Ann Surg, 1995, 222(3): 339-350.
2. 溫雨晴, 譚迎春, 張蓬波, 等. 胃轉流術對 2 型糖尿病大鼠脂肪組織胰島素抵抗的影響. 中華實驗外科雜志, 2013, 30(11): 2324-2326.
3. 張蓬波, 陳守坤, 張秀忠, 等. 胃轉流術對 2 型糖尿病大鼠肝組織胰島素受體底物-2、葡萄糖轉運體-2 表達的影響. 中華實驗外科雜志, 2013, 30(12): 2587-2589.
4. 張秀忠, 任澤強, 張蓬波. 胃轉流術對 2 型糖尿病大鼠的降糖作用及對糖耐量和胰島素抵抗的影響. 中華實驗外科雜志, 2010, 27(12): 1892-1894.
5. 龔帥, 任澤強, 張蓬波, 等. 胃轉流術對非肥胖型 2 型糖尿病大鼠骨骼肌胰島素受體底物-1 及泛素化蛋白表達的影響. 中國普外基礎與臨床雜志, 2016, 23(8): 915-921.
6. Shao J, Yamashita H, Qiao L, et al. Decreased Akt kinase activity and insulin resistance in C57BL/KsJ-Leprdb/db mice. J Endocrinol, 2000, 167(1): 107-115.
7. 譚柏宏, 張立海, 王躍生, 等. 胃轉流術對 2 型糖尿病大鼠棕色脂肪組織的影響. 黑龍江醫藥科學, 2018, 41(1): 39-40, 34.
8. Yu H, Li Q, Zhang M, et al. Decreased leptin is associated with alterations in thyroid-stimulating hormone levels after Roux-en-Y gastric bypass surgery in obese euthyroid patients with type 2 diabetes. Obes Facts, 2019, 12(3): 272-280.
9. 李晗, 高宏凱, 張新國, 等. 胃轉流手術后 GK 大鼠血糖變化與攝食量及體質量的關系. 中華實驗外科雜志, 2010, 27(8): 1069-1070.
10. 張強, 陳軒, 徐亮. 胃轉流術治療 2 型糖尿病與 JNK 信號通路的關系. 中國普外基礎與臨床雜志, 2018, 25(9): 1055-1059.
11. Xu J, Yang S, Gu X, et al. SPARC correlates with unfavorable outcome and promotes tumor growth in lung squamous cell carcinoma. Exp Mol Pathol, 2019, 110: 104276.
12. 黃海波, 趙燕, 楊樹軍. 富含半胱氨酸的酸性分泌蛋白在食管癌中的研究進展. 國際腫瘤學雜志, 2014, 41(12): 918-921.
13. 申玉翠, 張其勝, 姚憶蓉, 等. 結腸癌組織 SPARC 蛋白質的表達及其臨床意義. 實用醫學雜志, 2014, 30(20): 3277-3279.
14. 孫烈, 陳國衛, 尹杰, 等. 富含半胱氨酸的酸性分泌蛋白對胰腺癌細胞增殖、遷移和侵襲能力的影響. 中華實驗外科雜志, 2014, 31(9): 1954-1956.
15. Jiang X, Liu F, Wang Y, et al. Secreted protein acidic and rich in cysteine promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells and acquisition of cancer stem cell phenotypes. J Gastroenterol Hepatol, 2019, 34(10): 1860-1868.
16. Frangogiannis NG. Matricellular proteins in cardiac adaptation and disease. Physiol Rev, 2012, 92(2): 635-688.
17. 羅麗穎, 吳佳慧, 項瀟瓊, 等. 富含半胱氨酸的酸性分泌蛋白對青光眼濾過泡瘢痕化影響的研究進展. 上海交通大學學報: 醫學版, 2019, 39(6): 684-688.
18. 劉鍇, 宋海燕, 劉姝, 等. 2 型糖尿病肥胖患者 SPARC 表達差異性研究. 中華內分泌代謝雜志, 2014, 30(5): 388-392.
19. Takahashi M, Nagaretani H, Funahashi T, et al. The expression of SPARC in adipose tissue and its increased plasma concentration in patients with coronary artery disease. Obes Res, 2001, 9(7): 388-393.
20. Aoi W, Hirano N, Lassiter DG, et al. Secreted protein acidic and rich in cysteine (SPARC) improves glucose tolerance. FASEB J, 2019, 33(9): 10551-10562.
21. Tartare-Deckert S, Chavey C, Monthouel MN, et al. The matri-cellular protein SPARC/osteonectin as a newly identified factor up-regulated in obesity. J Biol Chem, 2001, 276(25): 22231-22237.
22. Nie J, Sage EH. SPARC inhibits adipogenesis by its enhancement of beta-catenin signaling. J Biol Chem, 2009, 284(2): 1279-1290.
23. Harries LW, McCulloch LJ, Holley JE, et al. A role for SPARC in the moderation of human insulin secretion. PLoS One, 2013, 8(6): e68253.
24. Song H, Guan Y, Zhang L, et al. SPARC interacts with AMPK and regulates GLUT4 expression. Biochem Biophys Res Commun, 2010, 396(4): 961-966.
25. Jing Y, Jin Y, Wang Y, et al. SPARC promotes the proliferation and metastasis of oral squamous cell carcinoma by PI3K/AKT/PDGFB/PDGFRβ axis. J Cell Physiol, 2019, [Epub ahead of print].

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Influence on the expression of SPARC and GLUT-4 protein in adipose tissue ofnon-obese GK rats after gastric bypass operation

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