Effects of Tiotropium Bromide on Inflammatory Factors in Serum, BALF and Lung Tissue of COPD Rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

ZhangJinmei ,  SunLihua , GuWei , TanYan , FangSurong

Department of Respiratory Medicine, Nanjing Hospital Affiliated to Nanjing Medical University(Nanjing First Hospital), Nanjing, Jiangsu, 210006, China;

Corresponding?author：

SunLihua, Email: nfhsun@sina.com

Keywords：

Tiotropium bromide; Chronic obstructive pulmonary disease; Rats; Inflammatory factors

DOI：

10.7507/1671-6205.2014115

Video：

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Objective To investigate the changes of interleukin-8 (IL-8), tumor necrosis factor-α(TNF-α) and phospholipase A2 (PLA2) in serum, bronchoalveolar lavage fluid (BALF) and lung tissue of COPD rats and the effects of tiotropium.To identify the anti-inflammatory function of tiotropium. Methods Rat COPD model was established by passive smoking as well as intratracheal instillation of lipopolysaccharide(LPS).Thirty male SD rats were randomly divided into a control group, a COPD group and a tiotropium bromide treatment group (n=10 in each group).The pathologic changes of the lung tissue and airway were observed by HE staining.The total and differential cell counts in BALF were observed.The levels of IL-8, TNF-α, PLA2 in serum, BALF and lung tissue were measured by enzyme-linked immunosorbent assay. Results HE staining revealed that the rat COPD model was successfully established.The COPD group appeared obvious emphysema, while the treatment group appeared mild emphysema.The total inflammatory cells, the proportion of neutrophils and lymphocyte, and the levels of IL-8, TNF-α, PLA2 in serum, BALF and lung tissue in the COPD group were obviously higher than those in the control group (P < 0.01).The total inflammatory cells, the proportion of neutrophils and lymphocyte, and the levels of IL-8, TNF-α, PLA2 in serum, BALF and lung tissue in the treatment group were significantly lower than those in the COPD group but higher than those in the control group (P < 0.01). Conclusions Tiotropium bromide can reduce the levels of IL-8, TNF-α, PLA2 in serum, BALF and lung tissue of COPD rats by reducing the leakage of inflammatory cells, and alleviate the airway inflammation and the degree of emphysema.

Citation： ZhangJinmei, SunLihua, GuWei, TanYan, FangSurong. Effects of Tiotropium Bromide on Inflammatory Factors in Serum, BALF and Lung Tissue of COPD Rats. Chinese Journal of Respiratory and Critical Care Medicine, 2014, 13(5): 469-473. doi: 10.7507/1671-6205.2014115 Copy

1.	Hanania NA, Marciniuk DD.A unified front against COPD:clinical practice guidelines from the American College of Physicians, the American College of Chest Physicians, the American Thoracic Society, and the European Respiratory Society.Chest, 2011, 140:565-566.
2.	何牡丹, 榮令, 周新.噻托溴銨在COPD大鼠氣道炎癥中的作用.中國呼吸與危重監護雜志, 2009, 8:212-215.
3.	單淑香, 陳寶元.慢性阻塞性肺疾病患者誘導痰中白介素8、白三烯B4、髓過氧化物酶的變化以及噻托溴銨對其的影響.國際呼吸雜志, 2010, 30:1153-1156.
4.	李紅梅, 崔德健, 佟欣, 等.熏香煙加氣管注內毒素和單純熏香煙法建立大鼠COPD模型.中國病理生理雜志, 2002:808-812.
5.	管小俊.肺內輸入骨髓間充質干細胞對肺氣腫大鼠慢性炎癥的調控.中國呼吸與危重監護雜志, 2011, 10:520-526.
6.	高潔, 王旭紅, 陳茜.皺肺平喘膠囊對COPD大鼠血清IL-8和TNF-α表達的影響.陜西中醫, 2009:1551-1553.
7.	田春燕, 張繼紅, 李偉國, 等.細胞因子與COPD炎癥機制研究進展.臨床肺科雜志, 2011, 16:248-249.
8.	吳紀珍, 馬利軍, 李素云, 等.慢性阻塞性肺疾病發作期與緩解期體內膠原、細胞因子變化及臨床意義.中國實用內科雜志, 2005, 25:703-704.
9.	陳麗, 劉升明.慢性阻塞性肺疾病的氣道炎癥及治療新進展.實用醫學雜志, 2008:4170-4171.
10.	Pourfarzam S, Ghazanfari T, Yaraee R, et al.Serum levels of IL-8 and IL-6 in the long term pulmonary complications induced by sulfur mustard:Sardasht-Iran Cohort Study.Int Immunopharmacol, 2009, 9:1482-1488.
11.	王紅陽, 趙雪峰, 李球兵, 等.COPD及其吸煙患者IL-8、TNF-α、hsCRP的檢測及意義.山東醫藥, 2007, 47:42-44.
12.	Wood LG, Gibson PG, Garg ML.Biomarkers of lipid peroxidation, airway inflammation and asthma.Eur Respir J, 2003, 21:177-186.
13.	Churg A, Dai J, Tai H, et al.Tumor necrosis factor-alpha is central to acute cigarette smoke-induced inflammation and connective tissue breakdown.Am J Respir Crit Care Med, 2002, 166:849-854.
14.	Mayer RJ, Marshall LA.New insights on mammalian phospholipase A2(s), comparison of arachidonoyl-selective and-nonselective enzymes.FASEB J, 1993, 7:339-348.
15.	Kim DK, Fukuda T, Thompson BT, et al.Bronchoalveolar lavage fluid phospholipase A2 activities are increased in human adult respiratory distress syndrome.Am J Physiol, 1995, 269(1 Pt 1):L109-L118.
16.	Currie GP, Rossiter C, Miles SA, et al.Effects of tiotropium and other long acting bronchodilators in chronic obstructive pulmonary disease.Pulm Pharmacol Ther, 2006, 19:112-119.
17.	李莉芳, 李建強.噻托溴銨在慢性阻塞性肺疾病大鼠模型氣道炎癥和重塑中的保護作用.山西醫藥雜志, 2008, 37:103-104.

1. Hanania NA, Marciniuk DD.A unified front against COPD:clinical practice guidelines from the American College of Physicians, the American College of Chest Physicians, the American Thoracic Society, and the European Respiratory Society.Chest, 2011, 140:565-566.
2. 何牡丹, 榮令, 周新.噻托溴銨在COPD大鼠氣道炎癥中的作用.中國呼吸與危重監護雜志, 2009, 8:212-215.
3. 單淑香, 陳寶元.慢性阻塞性肺疾病患者誘導痰中白介素8、白三烯B4、髓過氧化物酶的變化以及噻托溴銨對其的影響.國際呼吸雜志, 2010, 30:1153-1156.
4. 李紅梅, 崔德健, 佟欣, 等.熏香煙加氣管注內毒素和單純熏香煙法建立大鼠COPD模型.中國病理生理雜志, 2002:808-812.
5. 管小俊.肺內輸入骨髓間充質干細胞對肺氣腫大鼠慢性炎癥的調控.中國呼吸與危重監護雜志, 2011, 10:520-526.
6. 高潔, 王旭紅, 陳茜.皺肺平喘膠囊對COPD大鼠血清IL-8和TNF-α表達的影響.陜西中醫, 2009:1551-1553.
7. 田春燕, 張繼紅, 李偉國, 等.細胞因子與COPD炎癥機制研究進展.臨床肺科雜志, 2011, 16:248-249.
8. 吳紀珍, 馬利軍, 李素云, 等.慢性阻塞性肺疾病發作期與緩解期體內膠原、細胞因子變化及臨床意義.中國實用內科雜志, 2005, 25:703-704.
9. 陳麗, 劉升明.慢性阻塞性肺疾病的氣道炎癥及治療新進展.實用醫學雜志, 2008:4170-4171.
10. Pourfarzam S, Ghazanfari T, Yaraee R, et al.Serum levels of IL-8 and IL-6 in the long term pulmonary complications induced by sulfur mustard:Sardasht-Iran Cohort Study.Int Immunopharmacol, 2009, 9:1482-1488.
11. 王紅陽, 趙雪峰, 李球兵, 等.COPD及其吸煙患者IL-8、TNF-α、hsCRP的檢測及意義.山東醫藥, 2007, 47:42-44.
12. Wood LG, Gibson PG, Garg ML.Biomarkers of lipid peroxidation, airway inflammation and asthma.Eur Respir J, 2003, 21:177-186.
13. Churg A, Dai J, Tai H, et al.Tumor necrosis factor-alpha is central to acute cigarette smoke-induced inflammation and connective tissue breakdown.Am J Respir Crit Care Med, 2002, 166:849-854.
14. Mayer RJ, Marshall LA.New insights on mammalian phospholipase A2(s), comparison of arachidonoyl-selective and-nonselective enzymes.FASEB J, 1993, 7:339-348.
15. Kim DK, Fukuda T, Thompson BT, et al.Bronchoalveolar lavage fluid phospholipase A2 activities are increased in human adult respiratory distress syndrome.Am J Physiol, 1995, 269(1 Pt 1):L109-L118.
16. Currie GP, Rossiter C, Miles SA, et al.Effects of tiotropium and other long acting bronchodilators in chronic obstructive pulmonary disease.Pulm Pharmacol Ther, 2006, 19:112-119.
17. 李莉芳, 李建強.噻托溴銨在慢性阻塞性肺疾病大鼠模型氣道炎癥和重塑中的保護作用.山西醫藥雜志, 2008, 37:103-104.

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Effects of Tiotropium Bromide on Inflammatory Factors in Serum, BALF and Lung Tissue of COPD Rats

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