Expression and significance of S100A12 in peripheral blood of patients with acute respiratory distress syndrome_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

GAO Jiqing ¹ , GAO Mingtong ² , LI Laichuan ² , TAN Qiang ³ , CHEN Jingxia ² , LIU Meijuan ⁴ ,  SHI Youkui ²

1. Weifang Medical University, Weifang, Shandong 261042, P. R. China;
2. Department of Emergency Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P. R. China;
3. The Second Department of Respiratory Medicine, Weifang People's Hospital, Weifang, Shandong 261000, P. R. China;
4. Department of Respiratory Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P. R. China;

Corresponding?author：

SHI Youkui, Email: syk2101603@163.com

Keywords：

Acute respiratory distress syndrome; Calcium-binding protein; Prognosis

DOI：

10.7507/1671-6205.201704034

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Objective To investigate the expression of S100A12 protein in peripheral blood of patients with acute respiratory distress syndrome (ARDS) and its clinical significance. Methods Sixty ARDS patients admitted between October 2015 and December 2016 were included in the study. They were divided into a mild group (n=20), a moderate group (n=20) and a severe group (n=20) according to the oxygenation state. Meanwhile they were divided into a survival group (n=37) and a death group (n=23) according to the 30-day prognosis. Sixty simultaneous healthy subjects were selected as a control group. The clinical data of all subjects were collected. Fasting venous blood was collected in the morning for measurement of S100A12 expression in peripheral blood by ELISA method. Results Compared with the control group, the levels of S100A12, interleukin-1 (IL-1), tumor necrosis factor alpha (TNF-α) in peripheral blood and APACHEⅡ score were significantly increased in the ARDS patients, and PaO ₂/FiO ₂ was significantly decreased (all P<0.05). The level of S100A12 in peripheral blood was positively correlated with the severity of ARDS. The level of S100A12 was much higher in the severe group than that in the moderate group and the mild group, and higher in the moderate group than that in the mild group (allP<0.05). Correlation analysis showed that S100A12 level in peripheral blood of the ARDS patients was positively correlated with IL-1, TNF-α and APACHEⅡ score (P<0.05), but negatively correlated with PaO ₂/FiO ₂ (P<0.05). Logistic regression analysis showed that IL-1, TNF-α, APACHEⅡ score and S100A12 were independent risk factors for prognosis of ARDS. PaO ₂/FiO ₂ was an independent protective factor for prognosis of ARDS. ROC curve analysis showed that the S100A12 level had a certain predictive value for ARDS and could be used as a prognostic indicator. Conclusions The level of S100A12 in peripheral blood of ARDS patients is significantly increased and is closely related to the severity of ARDS. It has a potential clinical value for early diagnosis, treatment and prognosis of ARDS.

Citation： GAO Jiqing, GAO Mingtong, LI Laichuan, TAN Qiang, CHEN Jingxia, LIU Meijuan, SHI Youkui. Expression and significance of S100A12 in peripheral blood of patients with acute respiratory distress syndrome. Chinese Journal of Respiratory and Critical Care Medicine, 2017, 16(5): 457-461. doi: 10.7507/1671-6205.201704034 Copy

1.	徐采云. 轉錄因子 SP1 參與 TNF-α 介導的肺泡上皮細胞氧化損傷中 Nox1 基因的表達調控[D].南方醫科大學, 2013.
2.	Takaoka Y, Goto S, Nakano T, et al. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) prevents lipopolysaccharide(LPS)-induced, sepsis-related severe acute lung injury in mice. Sci Rep, 2014, 4, 5204.
3.	Matthay MA, Zimmerman GA, Esmon C, et al. Future research directions in acute lung injury: summary of a National Heart, Lung, and Blood Institute working group. Am J Respir Crit Care Med, 2003, 167(7): 1027-1035.
4.	Bellingan GJ. The pulmonary physician in critical care * 6: The pathogenesis of ALI/ARDS. Thorax. 2002, 57(6), 540-546.
5.	Hotchkiss RS, Karl IE. The pathophysiology and treatment of sepsis. N Engl J Med, 2003, 348(2): 138-150.
6.	Goodman RB, Pugin J, Lee JS, et al. Cytokine-mediated inflammation in acute lung injury. Cytokine Growth Factor Rev, 2003, 14(6): 523-535.
7.	de Torre C, Ying SX, Munson PJ, et al. Proteomic analysis of inflammatory biomarkers in bronchoalveolar lavage. Proteomics, 2006, 6(13): 3949-3957.
8.	董海云, 向旭東. S100 蛋白與急性肺損傷/急性呼吸窘迫綜合征. 內科急危重癥雜志, 2010, 16(5): 269-271.0.
9.	代鳳雷, 孫強, 張斌, 等. 髖骨性關節炎滑膜組織中鈣結合蛋白(S100A12)的表達及意義. 中國骨質疏松雜志, 2017, 23(1): 27-30.0.
10.	陳榮國, 代鳳雷, 歐先鋒, 等. 血清和關節液中鈣結合蛋白(S100A12)的水平與膝關節骨關節炎嚴重程度的相關性分析. 中國骨質疏松雜志, 2016, 22(8): 985-988.0.
11.	申宇站, 路勇明. 血清與糞便 S100A12 蛋白在新生兒壞死性小腸結腸炎診斷及臨床分期中的應用研究. 中外醫學研究, 2016, 14(18): 46-47.0.
12.	林松挺. 血清 S100A12 對潰瘍性結腸炎患者的診斷和疾病評估價值. 世界華人消化雜志, 2016, 24(7): 1103-1106.0.
13.	李慕然, 劉艷迪. 鈣結合蛋白 S100A12 的臨床研究進展. 醫學綜述, 2012, 18(24): 4161-4163.0.
14.	鄧秋菊, 蔣曉靜. 慢性心力衰竭患者鈣結合蛋白 S100A12 的表達意義及機制分析. 河北醫學, 2017, 23(1): 38-410.
15.	ARDS Definition Task Force, Ranieri VM, Rubenfeld GD, Thompson BT, et al. Acute respiratory distress syndrome: the Berlin Definition. JAMA, 2012, 307(23): 2526-2533.
16.	王辰. 急性呼吸窘迫綜合征. 見: 葛均波, 徐永健, 主編. 內科學. 第 8 版. 北京: 人民衛生出版社, 2013, 133-137.
17.	陳彥. 臨床常用移植保存介質對臍帶間充質干細胞生物學特性與體內治療效果的影響及其機制的初步探討[D]. 山西醫科大學, 2012.
18.	但小蘋, 肖貞良. 細胞膜微粒在急性呼吸窘迫綜合征中作用的研究現狀. 中國呼吸與危重監護雜志, 2015, 14(4): 413-416.0.
19.	Kang JH, Hwang SM, Chung IY. S100A8, S100A9 and S100A12 activate airway epithelial cells to produce MUC5AC via extracellular signal-regulated kinase and nuclear factor-κB pathways. Immunology, 2015, 144(1): 79-90.
20.	范曉枝, 劉宏. 炎性因子在急性呼吸窘迫綜合征發病中的作用. 國外醫學(醫學地理分冊), 2015, 36(4): 314-317.0.
21.	Lorenz E, Muhlebach MS, Tessier PA, et al. Different expression ratio of S100A8/A9 and S100A12 in acute and chronic lung diseases. Respir Med, 2008, 102(4): 567-573.
22.	盧善翃, 李俊輝, 歐陽莎, 等. 重癥病毒性肺炎合并急性呼吸窘迫綜合征的預后危險因素分析. 中國呼吸與危重監護雜志, 2014, 13(6): 560-564.0.
23.	楊毅, 邱海波. 急性呼吸窘迫綜合征過去、現在、未來. 中國實用內科雜志, 2013, 33(11): 833-837.0.

1. 徐采云. 轉錄因子 SP1 參與 TNF-α 介導的肺泡上皮細胞氧化損傷中 Nox1 基因的表達調控[D].南方醫科大學, 2013.
2. Takaoka Y, Goto S, Nakano T, et al. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) prevents lipopolysaccharide(LPS)-induced, sepsis-related severe acute lung injury in mice. Sci Rep, 2014, 4, 5204.
3. Matthay MA, Zimmerman GA, Esmon C, et al. Future research directions in acute lung injury: summary of a National Heart, Lung, and Blood Institute working group. Am J Respir Crit Care Med, 2003, 167(7): 1027-1035.
4. Bellingan GJ. The pulmonary physician in critical care * 6: The pathogenesis of ALI/ARDS. Thorax. 2002, 57(6), 540-546.
5. Hotchkiss RS, Karl IE. The pathophysiology and treatment of sepsis. N Engl J Med, 2003, 348(2): 138-150.
6. Goodman RB, Pugin J, Lee JS, et al. Cytokine-mediated inflammation in acute lung injury. Cytokine Growth Factor Rev, 2003, 14(6): 523-535.
7. de Torre C, Ying SX, Munson PJ, et al. Proteomic analysis of inflammatory biomarkers in bronchoalveolar lavage. Proteomics, 2006, 6(13): 3949-3957.
8. 董海云, 向旭東. S100 蛋白與急性肺損傷/急性呼吸窘迫綜合征. 內科急危重癥雜志, 2010, 16(5): 269-271.0.
9. 代鳳雷, 孫強, 張斌, 等. 髖骨性關節炎滑膜組織中鈣結合蛋白(S100A12)的表達及意義. 中國骨質疏松雜志, 2017, 23(1): 27-30.0.
10. 陳榮國, 代鳳雷, 歐先鋒, 等. 血清和關節液中鈣結合蛋白(S100A12)的水平與膝關節骨關節炎嚴重程度的相關性分析. 中國骨質疏松雜志, 2016, 22(8): 985-988.0.
11. 申宇站, 路勇明. 血清與糞便 S100A12 蛋白在新生兒壞死性小腸結腸炎診斷及臨床分期中的應用研究. 中外醫學研究, 2016, 14(18): 46-47.0.
12. 林松挺. 血清 S100A12 對潰瘍性結腸炎患者的診斷和疾病評估價值. 世界華人消化雜志, 2016, 24(7): 1103-1106.0.
13. 李慕然, 劉艷迪. 鈣結合蛋白 S100A12 的臨床研究進展. 醫學綜述, 2012, 18(24): 4161-4163.0.
14. 鄧秋菊, 蔣曉靜. 慢性心力衰竭患者鈣結合蛋白 S100A12 的表達意義及機制分析. 河北醫學, 2017, 23(1): 38-410.
15. ARDS Definition Task Force, Ranieri VM, Rubenfeld GD, Thompson BT, et al. Acute respiratory distress syndrome: the Berlin Definition. JAMA, 2012, 307(23): 2526-2533.
16. 王辰. 急性呼吸窘迫綜合征. 見: 葛均波, 徐永健, 主編. 內科學. 第 8 版. 北京: 人民衛生出版社, 2013, 133-137.
17. 陳彥. 臨床常用移植保存介質對臍帶間充質干細胞生物學特性與體內治療效果的影響及其機制的初步探討[D]. 山西醫科大學, 2012.
18. 但小蘋, 肖貞良. 細胞膜微粒在急性呼吸窘迫綜合征中作用的研究現狀. 中國呼吸與危重監護雜志, 2015, 14(4): 413-416.0.
19. Kang JH, Hwang SM, Chung IY. S100A8, S100A9 and S100A12 activate airway epithelial cells to produce MUC5AC via extracellular signal-regulated kinase and nuclear factor-κB pathways. Immunology, 2015, 144(1): 79-90.
20. 范曉枝, 劉宏. 炎性因子在急性呼吸窘迫綜合征發病中的作用. 國外醫學(醫學地理分冊), 2015, 36(4): 314-317.0.
21. Lorenz E, Muhlebach MS, Tessier PA, et al. Different expression ratio of S100A8/A9 and S100A12 in acute and chronic lung diseases. Respir Med, 2008, 102(4): 567-573.
22. 盧善翃, 李俊輝, 歐陽莎, 等. 重癥病毒性肺炎合并急性呼吸窘迫綜合征的預后危險因素分析. 中國呼吸與危重監護雜志, 2014, 13(6): 560-564.0.
23. 楊毅, 邱海波. 急性呼吸窘迫綜合征過去、現在、未來. 中國實用內科雜志, 2013, 33(11): 833-837.0.

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Expression and significance of S100A12 in peripheral blood of patients with acute respiratory distress syndrome

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