Effect of ampelopsin in mice with bleomycin-induced pulmonary fibrosis_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

HUANG Haitao ^1,2 , FANF Jing ¹ , ZENG Chunhui ¹ , YIN Xijing ¹ , HONG Zhengshan ¹ , TAN Liuping ¹ ,  YANG Ke ^1,2

1. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, P. R. China;
2. Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning, Guangxi 530020, P. R. China;

Corresponding?author：

YANG Ke, Email: kyang13481020102@126.com

Keywords：

Pulmonary fibrosis; Ampelopsin; Respiratory function; In vivo; Bleomycin

DOI：

10.7507/1671-6205.201903086

Video：

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Objective This study construct a pulmonary fibrosis model in vivo to study anti-pulmonary fibrosis effect of ampelopsis.Methods We constructed a pulmonary fibrosis model by bleomycin in BALB/c mice. The mice were divided by weight random number table into a blank control group, a model control group, a dexamethasone treatment group (intervened with dexamethasone in a dose of 2.5 mg/kg), and three ampelopsis treatment groups intervened with ampelopsis in dose of 200, 100, and 50 mg/kg, respectively. Bleomycin solution (3 mg/kg) was intratracheally injected respectively on 1^st and 14^th day, except the blank group. Twenty-eight days later, the relevant indicators were collected, including respiratory function (airway resistance, dynamic lung compliance, maximal ventilator volume), level of hydroxyproline and histopathological changes in the lungs.Results After 28 days, the model control group mice had severe respiratory resistance, dynamic lung compliance and maximal ventilator volume were decreased. The high dose ampelopsis treatment could enhance respiratory function (P<0.05). Lung coefficient was lower in the treatment groups than that in the model control group (P<0.05). The hydroxyproline of the treatment groups was less than that of the model control group (P<0.05). Histopathological examination showed that the degree of fibrosis increased in the model control group (P<0.05), but decreased in the treatment groups (P<0.05).Conclusion Ampelopsis can resist bleomycin-induced pulmonary fibrosis in mice, relieve the symptoms of respiratory failure, reduce the formation of collagen, and produce anti-pulmonary fibrosis effect.

Citation： HUANG Haitao, FANF Jing, ZENG Chunhui, YIN Xijing, HONG Zhengshan, TAN Liuping, YANG Ke. Effect of ampelopsin in mice with bleomycin-induced pulmonary fibrosis. Chinese Journal of Respiratory and Critical Care Medicine, 2020, 19(3): 270-275. doi: 10.7507/1671-6205.201903086 Copy

1.	黃海濤, 唐藝文, 楊柯. 肺纖維化信號調控通路的研究進展. 國際呼吸雜志, 2018, 38(4): 311-314.
2.	徐冰珠, 康建磊. 特發性肺纖維化治療藥物的研究進展. 現代藥物與臨床, 2015, 30(7): 897-902.
3.	Mouratis MA, Aidinis V. Modeling pulmonary fibrosis with bleomycin. Curr Opin Pulm Med, 2011, 17(5): 355-361.
4.	蘇素嬌. 顯齒蛇葡萄品質評價及其相關藥效學研究[D]. 福建中醫藥大學, 2014.
5.	中國科學院植物研究所. 中國高等植物圖鑒(第二冊)補編. 北京: 科學出版社, 1983, 349-356.
6.	謝郁峰, 陳卓瀚, 呂華沖. 響應面分析法優化藤茶中二氫楊梅素的提取工藝. 中國實驗方劑學雜志, 2012, 18(17): 46-49.
7.	張友勝, 寧正祥, 楊書珍, 等. 顯齒蛇葡萄中二氫楊梅樹皮素的抗氧化作用及其機制(英文). 藥學學報, 2003, 38(4): 241-244.
8.	閻莉, 衛智權, 鄭作文, 等. 廣西藤茶雙氫楊梅樹皮素對小鼠肝細胞凋亡的影響. 中國實驗方劑學雜志, 2010, 16(16): 102-104.
9.	Ashcroft T, Simpson JM, Timbrell V. Simple method of estimating severity of pulmonary fibrosis on a numerical scale. J Clin Pathol, 1988, 41(4): 467.
10.	Wilson MS, Wynn TA. Pulmonary fibrosis: pathogenesis, etiology and regulation. Mucosal Immunol, 2009, 2(2): 103-121.
11.	邢小燕, 李光偉. 類固醇糖尿病——糖皮質激素治療中必須警惕的不良反應. 中華風濕病學雜志, 2009, 13(12): 801-803.
12.	Tan WJ, Tan QY, Wang T, et al. Calpain 1 regulates TGF-β1-induced epithelial-mesenchymal transition in human lung epithelial cells via PI3K/Akt signaling pathway. Am J Transl Res, 2017, 9(3): 1402-1409.
13.	Moore BB, Hogaboam CM. Murine models of pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol, 2008, 294(2): L152-L160.
14.	劉虹, 李轍, 呂曉希, 等. 中和 IL-17A 活性改善肺纖維化小鼠肺功能. 航天醫學與醫學工程, 2013, 26(1): 38-42.
15.	中華醫學會呼吸病學分會肺功能專業組. 肺功能檢查指南(第二部分)——肺量計檢查. 中華結核和呼吸雜志, 2014, 37(7): 481-486.
16.	劉加良, 施廣懿. 脈沖振蕩與通氣肺功能二種檢測方法比較. 浙江預防醫學, 2002, 14(11): 6-8.
17.	魯惠順, 衛木根, 胡方. 潮氣量與肺順應性、呼氣第一秒容量及壓力—容量的相關性. 浙江臨床醫學, 2001, 3(11): 812-813.
18.	方賢磊, 李楊, 聶李亞, 等. 肺功能分析在放射性肺損傷防治藥物藥效評價中的作用. 軍事醫學, 2015, 39(7): 519-522.
19.	楊秀穎, 杜冠華. 組織羥脯氨酸含量微量測定方法及應用. 中國藥理學通報, 2004, 20(7): 836-837.
20.	謝偉娜, 丁奇, 孫靜, 等. 百部湯對博來霉素誘導小鼠肺纖維化的抑制作用. 中國藥科大學學報, 2018, 49(4): 483-489.
21.	Chen C, Wang YY, Wang YX, et al. Gentiopicroside ameliorates bleomycin-induced pulmonary fibrosis in mice via inhibiting inflammatory and fibrotic process. Biochem Biophys Res Commun, 2018, 495(4): 2396-2403.
22.	林藝凱, 馬愛平, 周偉躍, 等. mTOR 信號通路在博來霉素誘導小鼠肺纖維化中的作用機制研究. 中國呼吸與危重監護雜志, 2018, 17(2): 178-182.
23.	馬愛平, 高云周, 蘭文斌, 等. PI3K/Akt/HIF-1α 信號通路在博來霉素誘導小鼠肺纖維化中的作用機制研究. 中國呼吸與危重監護雜志, 2016, 15(1): 34-38.

1. 黃海濤, 唐藝文, 楊柯. 肺纖維化信號調控通路的研究進展. 國際呼吸雜志, 2018, 38(4): 311-314.
2. 徐冰珠, 康建磊. 特發性肺纖維化治療藥物的研究進展. 現代藥物與臨床, 2015, 30(7): 897-902.
3. Mouratis MA, Aidinis V. Modeling pulmonary fibrosis with bleomycin. Curr Opin Pulm Med, 2011, 17(5): 355-361.
4. 蘇素嬌. 顯齒蛇葡萄品質評價及其相關藥效學研究[D]. 福建中醫藥大學, 2014.
5. 中國科學院植物研究所. 中國高等植物圖鑒(第二冊)補編. 北京: 科學出版社, 1983, 349-356.
6. 謝郁峰, 陳卓瀚, 呂華沖. 響應面分析法優化藤茶中二氫楊梅素的提取工藝. 中國實驗方劑學雜志, 2012, 18(17): 46-49.
7. 張友勝, 寧正祥, 楊書珍, 等. 顯齒蛇葡萄中二氫楊梅樹皮素的抗氧化作用及其機制(英文). 藥學學報, 2003, 38(4): 241-244.
8. 閻莉, 衛智權, 鄭作文, 等. 廣西藤茶雙氫楊梅樹皮素對小鼠肝細胞凋亡的影響. 中國實驗方劑學雜志, 2010, 16(16): 102-104.
9. Ashcroft T, Simpson JM, Timbrell V. Simple method of estimating severity of pulmonary fibrosis on a numerical scale. J Clin Pathol, 1988, 41(4): 467.
10. Wilson MS, Wynn TA. Pulmonary fibrosis: pathogenesis, etiology and regulation. Mucosal Immunol, 2009, 2(2): 103-121.
11. 邢小燕, 李光偉. 類固醇糖尿病——糖皮質激素治療中必須警惕的不良反應. 中華風濕病學雜志, 2009, 13(12): 801-803.
12. Tan WJ, Tan QY, Wang T, et al. Calpain 1 regulates TGF-β1-induced epithelial-mesenchymal transition in human lung epithelial cells via PI3K/Akt signaling pathway. Am J Transl Res, 2017, 9(3): 1402-1409.
13. Moore BB, Hogaboam CM. Murine models of pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol, 2008, 294(2): L152-L160.
14. 劉虹, 李轍, 呂曉希, 等. 中和 IL-17A 活性改善肺纖維化小鼠肺功能. 航天醫學與醫學工程, 2013, 26(1): 38-42.
15. 中華醫學會呼吸病學分會肺功能專業組. 肺功能檢查指南(第二部分)——肺量計檢查. 中華結核和呼吸雜志, 2014, 37(7): 481-486.
16. 劉加良, 施廣懿. 脈沖振蕩與通氣肺功能二種檢測方法比較. 浙江預防醫學, 2002, 14(11): 6-8.
17. 魯惠順, 衛木根, 胡方. 潮氣量與肺順應性、呼氣第一秒容量及壓力—容量的相關性. 浙江臨床醫學, 2001, 3(11): 812-813.
18. 方賢磊, 李楊, 聶李亞, 等. 肺功能分析在放射性肺損傷防治藥物藥效評價中的作用. 軍事醫學, 2015, 39(7): 519-522.
19. 楊秀穎, 杜冠華. 組織羥脯氨酸含量微量測定方法及應用. 中國藥理學通報, 2004, 20(7): 836-837.
20. 謝偉娜, 丁奇, 孫靜, 等. 百部湯對博來霉素誘導小鼠肺纖維化的抑制作用. 中國藥科大學學報, 2018, 49(4): 483-489.
21. Chen C, Wang YY, Wang YX, et al. Gentiopicroside ameliorates bleomycin-induced pulmonary fibrosis in mice via inhibiting inflammatory and fibrotic process. Biochem Biophys Res Commun, 2018, 495(4): 2396-2403.
22. 林藝凱, 馬愛平, 周偉躍, 等. mTOR 信號通路在博來霉素誘導小鼠肺纖維化中的作用機制研究. 中國呼吸與危重監護雜志, 2018, 17(2): 178-182.
23. 馬愛平, 高云周, 蘭文斌, 等. PI3K/Akt/HIF-1α 信號通路在博來霉素誘導小鼠肺纖維化中的作用機制研究. 中國呼吸與危重監護雜志, 2016, 15(1): 34-38.

Chinese Journal of Respiratory and Critical Care Medicine

Effect of ampelopsin in mice with bleomycin-induced pulmonary fibrosis

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