Protective effect of ophiopogon japonicus saponin D on lipopolysaccharide induced acute lung injury in mice_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

 XU Lingwen , WANG Huabing , JIE Fengying , DENG Shuping , DONG Fang

Department of Critical Care Medicine, Wuhan Third Hospital, Wuhan, Hubei 430060, P. R. China;

Corresponding?author：

XU Lingwen, Email: 2848251357@qq.com

Keywords：

Acute lung injury; Lipopolysaccharide; Ophiopogonin D; Th17/Treg cells

DOI：

10.7507/1671-6205.202007004

Video：

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Objective To study the protective effect and mechanism of ophiopogonin D (OP-D) on lipopolysaccharide induced acute lung injury (ALI) in mice.Methods Fifty SPF C57BL/6 mice were randomly divided into five groups, ie. a control group, a sham operation group, a model group, an OP-D group (10 mg·kg^–1·d^–1), and a dexamethasone group (2 mg·kg^–1·d^–1), with 10 mice in each group. One day before the establishment of the model, the OP-D group and the dexamethasone group received the corresponding drugs by gavage. The model group, the OP-D group and the dexamethasone group received lipopolysaccharide (2 mg/kg, 30 μL) through the trachea to establish the ALI model. The sham operation group received the same volume of normal saline. The blank control group was not treated. Six hours after the operation, the mice were weighed and then killed for peripheral blood and lung tissue. The weight of lung tissue was measured to evaluate the degree of pulmonary edema; the pathological changes of lung tissue were observed by hematoxylin-eosin staining; the mRNA expressions of interleukin (IL)-6, IL-10, and IL-17 in lung tissue were detected by qPCR; the percentage of Th17 and Treg cells in peripheral blood was detected by flow cytometry.Results Compared with the model group, the degree of pulmonary edema in the OP-D group decreased significantly (P<0.05), the lung tissue injury decreased, the mRNA expressions of IL-6 and IL-17 in the lung tissue and the proportion of Th17 cells in the peripheral blood decreased significantly (P<0.05), the proportion of Treg cells in the peripheral blood and the mRNA expression of IL-10 in the lung tissue increased significantly (P<0.05).Conclusion OP-D may have therapeutic effect on LPS induced ALI in mice by regulating the balance of Th17/Treg cells.

Citation： XU Lingwen, WANG Huabing, JIE Fengying, DENG Shuping, DONG Fang. Protective effect of ophiopogon japonicus saponin D on lipopolysaccharide induced acute lung injury in mice. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(7): 503-508. doi: 10.7507/1671-6205.202007004 Copy

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4.	宋麗娟, 朱煜, 金鳴. 羥基紅花黃色素 A 抑制脂多糖誘導的急性肺損傷小鼠炎癥因子表達的研究. 中國呼吸與危重監護雜志, 2016, 15(6): 577-582.
5.	喻敏. 沙參麥冬湯加減應用于慢性支氣管炎的效果及對患者血清肺表面活性蛋白的影響. 臨床醫學研究與實踐, 2018, 3(17): 114-115.
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10.	呂濤, 李延倉, 李樹仁, 等. 依達拉奉對脂多糖引起的小鼠急性肺損傷的保護效應研究. 大連醫科大學學報, 2012, 34(4): 343-347.
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19.	袁林. Th17/Treg 在毛細支氣管炎發病機制中的作用. 國際兒科學雜志, 2017, 44(10): 697-700.
20.	Zhu J. The research of Treg cells: progress and challenge. World J Cardiovascr Dis, 2015, 5(6): 150-165.
21.	莊文珺, 謝炎, 顧劍玲, 等. 調節性 T 細胞在間接性急性肺損傷小鼠中的作用與機制. 中華結核和呼吸雜志, 2018, 41(12): 959-966.
22.	王瑩. 麥冬皂苷 D 通過激活 AMPK/NF-κB 信號通路緩解 PM2.5 誘導的肺泡上皮細胞炎癥反應[D]. 吉林大學, 2019.

1. 李旭, 陳巖, 白雪松, 等. 中藥治療急性肺損傷/急性呼吸窘迫綜合征作用機制研究進展. 遼寧中醫藥大學學報, 2017, 19(1): 166-170.
2. 伍桂雄, 朱水泉, 董樹烽, 等. COPD 急性加重期患者治療前后血清 IL-17 Th17 Treg 細胞變化及其與肺功能的相關性研究. 河北醫學, 2020, 26(1): 13-18.
3. 秦翌佳, 老啟芳, 黃冰, 等. 白細胞介素-17 對脂多糖致急性肺損傷小鼠炎癥免疫調節的影響. 中華危重病急救醫學, 2019, 31(8): 983-988.
4. 宋麗娟, 朱煜, 金鳴. 羥基紅花黃色素 A 抑制脂多糖誘導的急性肺損傷小鼠炎癥因子表達的研究. 中國呼吸與危重監護雜志, 2016, 15(6): 577-582.
5. 喻敏. 沙參麥冬湯加減應用于慢性支氣管炎的效果及對患者血清肺表面活性蛋白的影響. 臨床醫學研究與實踐, 2018, 3(17): 114-115.
6. 陳哲, 馮佳悅, 曹誠, 等. 襄麥冬多糖及皂苷體外活性研究. 食品研究與開發, 2018, 39(13): 7-12.
7. 王佳, 王寧寧, 葛運炫, 等. 麥冬皂苷 D 干預內質網應激減輕麥冬皂苷 D’所致心肌細胞損傷. 中國中藥雜志, 2019, 44(9): 1876-1881.
8. Ma C, Long H, Yang C, et al. Anti-inflammatory role of pilose antler peptide in LPS-induced lung injury. Inflammation, 2017, 40(3): 904-912.
9. Zhang YY, Meng C, Zhang XM, et al. Ophiopogonin D attenuates doxorubicin-induced autophagic cell death by relieving mitochondrial damage in vitro and in vivo. J Pharmacol Exp Ther, 2014, 352(1): 166-174.
10. 呂濤, 李延倉, 李樹仁, 等. 依達拉奉對脂多糖引起的小鼠急性肺損傷的保護效應研究. 大連醫科大學學報, 2012, 34(4): 343-347.
11. 程超. 麥冬果實藍色素的抗氧化特性研究. 中國釀造, 2009, 205(4): 62-64.
12. 王佳佳, 盧宗亮, 孔亞, 等. 麥冬皂苷 D’通過 RIP1/MLKL 誘導前列腺癌 PC3 細胞程序性壞死. 第三軍醫大學學報, 2017, 39(3): 201-207.
13. Song J, Suh YJ, Lee HJ, et al. Flecainide improve sepsis induced acute lung injury by controlling inflammatory response. Crit Care Med, 2016, 31(3): 194-201.
14. Knochelmann HM, Dwyer CJ, Bailey SR, et al. When worlds collide: Th17 and Treg cells in cancer and autoimmunity. Cell Mol Immunol, 2018, 15(5): 458-469.
15. Cheng L, Zhao Y, Qi D, et al. Wnt/β-catenin pathway promotes acute lung injury induced by LPS through driving the Th17 response in mice. Biochem Biophys Res Commun, 2018, 495(2): 1890-1895.
16. 齊欣欣, 應旭旻. 中醫藥對 Treg 細胞和 Th17 細胞分化的研究進展. 浙江中西醫結合雜志, 2016, 26(3): 298-301.
17. Hirahara K, Nakayama T. CD4⁺ T-cell subsets in inflammatory diseases: beyond the T_h1/T_h2 paradigm. Int Immunol, 2016, 28(4): 163-171.
18. 張海萍, 李芳. 調節性 T 細胞分化和功能調節機制的研究進展. 免疫學雜志, 2019, 35(12): 1100-1104.
19. 袁林. Th17/Treg 在毛細支氣管炎發病機制中的作用. 國際兒科學雜志, 2017, 44(10): 697-700.
20. Zhu J. The research of Treg cells: progress and challenge. World J Cardiovascr Dis, 2015, 5(6): 150-165.
21. 莊文珺, 謝炎, 顧劍玲, 等. 調節性 T 細胞在間接性急性肺損傷小鼠中的作用與機制. 中華結核和呼吸雜志, 2018, 41(12): 959-966.
22. 王瑩. 麥冬皂苷 D 通過激活 AMPK/NF-κB 信號通路緩解 PM2.5 誘導的肺泡上皮細胞炎癥反應[D]. 吉林大學, 2019.

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Protective effect of ophiopogon japonicus saponin D on lipopolysaccharide induced acute lung injury in mice

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