Study on the biological characteristics of umbilical cord mesenchymal stem cells and their reparative effects on bleomycin-induced acute lung injury in mice_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WEN Hebao ^1,3 , YE Wenhao ¹ , ZOU Xiaoyun ¹ , MA Caiyun ¹ , LIU Chunbo ¹ , LI xiangchen ² , LIU Changqing ¹ ,  GUAN Weijun ²

1. Anhui Engineering Research Center for Neural Regeneration Technology and Medical New Materials, Bengbu Medical University, Bengbu, Anhui, 233000, P.R. China;
2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;
3. Department of Sports and Art, Bengbu Medical University, Bengbu, Anhui, 233000, P.R. China;

Corresponding?author：

GUAN Weijun, Email: wj_guan301@126.com

Keywords：

Umbilical cord mesenchymal stem cells; multi-differentiation potential; transplantation therapy; lung injury; repair effect

DOI：

10.7507/1671-6205.202407026

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the multi-directional differentiation potential and other biological characteristics of chicken umbilical cord mesenchymal stem cells (UMSC), as well as their reparative effects on bleomycin (BLM)-induced lung injury in mice. Methods An acute lung injury model in mice was established by injecting BLM into the bronchus. UMSC were then transplanted via the tail vein. The reparative effects of UMSC on lung injury were evaluated through pathological section observation, survival and differentiation of transplanted cells in mice, and detection of hydroxyproline (HYP) content, among other indicators. Results The UMSC successfully isolated in this study positively expressed specific surface markers CD29, CD44, CD90, and CD166, while the expression of CD34 and CD45 was negative. Induced UMSC could differentiate into adipocytes, osteocytes, chondrocytes, and alveolar epithelial cells. Animal experiments revealed that BLM-treated mice exhibited damaged alveolar structures, significant inflammatory cell infiltration, abnormal collagen deposition, and pulmonary fibrosis. However, after UMSC transplantation, the extent and severity of lung damage were reduced, and the HYP content in lung tissue decreased but remained higher than that of the control group. Conclusion UMSC can continuously proliferate and maintain their biological characteristics under in vitro culture conditions. They possess the ability to migrate to damaged sites and undergo directional differentiation, demonstrating a certain reparative effect on BLM-induced acute lung injury in mice.

Citation： WEN Hebao, YE Wenhao, ZOU Xiaoyun, MA Caiyun, LIU Chunbo, LI xiangchen, LIU Changqing, GUAN Weijun. Study on the biological characteristics of umbilical cord mesenchymal stem cells and their reparative effects on bleomycin-induced acute lung injury in mice. Chinese Journal of Respiratory and Critical Care Medicine, 2025, 24(4): 260-267. doi: 10.7507/1671-6205.202407026 Copy

1.	Yuan D, Bao Y, El-Hashash A. Mesenchymal stromal cell-based therapy in lung diseases; from research to clinic. Am J Stem Cells, 2024, 13(2): 37-58.
2.	朱潔云, 高敏, 葉長廣, 等. 慢性阻塞性肺疾病患者一年非計劃再入院風險預測模型的構建. 中國呼吸與危重監護雜志, 2025, 24(1): 1-8.
3.	曾黎靜, 張嘉瑞, 易群. 慢性阻塞性肺疾病急性加重期合并II型呼吸衰竭列線圖模型的構建及驗證. 中國呼吸與危重監護雜志. 2024, 23 (12): 876-881.
4.	Ma J, Li G, Wang H, et al. Comprehensive review of potential drugs with anti-pulmonary fibrosis properties. Biomed Pharmacother, 2024, 173: 116282.
5.	Bai X, Chen Q, Li F, et al. Optimized inhaled LNP formulation for enhanced treatment of idiopathic pulmonary fibrosis via mRNA-mediated antibody therapy. Nat Commun, 2024, 15(1): 6844.
6.	Wang Y, Wang L, Ma S, et al. Repair and regeneration of the alveolar epithelium in lung injury. FASEB J. 2024, 38(8): e23612.
7.	Brown C, McKee C, Bakshi S, et al. Mesenchymal stem cells: Cell therapy and regeneration potential. J Tissue Eng Regen Med, 2019, 13(9): 1738-1755.
8.	Shaikh MS, Shahzad Z, Tash EA, et al. Human Umbilical Cord Mesenchymal Stem Cells: Current Literature and Role in Periodontal Regeneration. Cells, 2022, 11(7): 1168.
9.	毛壯, 王華. 間充質干細胞治療肺損傷研究進展. 軍事醫學, 2023, 4(9): 714-720.
10.	趙培, 郭石平. 黑色素瘤缺乏因子2炎癥小體在非感染性肺部疾病中的研究進展. 中國呼吸與危重監護雜志, 2025, 24(2): 147-152.
11.	Saito A, Horie M, Micke P, et al. The Role of TGF-β Signaling in Lung Cancer Associated with Idiopathic Pulmonary Fibrosis. Int J Mol Sci, 2018, 19(11): 3611.
12.	Weis VG, Deal AC, Mekkey G, et al. Human placental-derived stem cell therapy ameliorates experimental necrotizing enterocolitis. Am J Physiol Gastrointest Liver Physiol, 2021, 320(4): G658-G674.
13.	Liu N, Cheng Y, Wang D, et al. Tissue-specific populations from amniotic fluid-derived mesenchymal stem cells manifest variant in vitro and in vivo properties. Hum Cell, 2024, 37(2): 408-419.
14.	Fong CY, Chak LL, Subramanian A, et al. A three dimensional anchorage independent in vitro system for the prolonged growth of embryoid bodies to study cancer cell behaviour and anticancer agents. Stem Cell Rev Rep, 2009, 5(4): 410-9.
15.	Ayuzawa R, Doi C, Rachakatla RS, et al. Naive human umbilical cord matrix derived stem cells significantly attenuate growth of human breast cancer cells in vitro and in vivo. Cancer Lett, 2009, 280(1): 31-7.
16.	Chen MY, Lie PC, Li ZL, et al. Endothelial differentiation of Wharton's jelly-derived mesenchymal stem cells in comparison with bone marrow-derived mesenchymal stem cells. Exp Hematol, 2009, 37(5): 629-40.
17.	Mitchell KE, Weiss ML, Mitchell BM, et al. Matrix cells from Wharton's jelly form neurons and glia. Stem Cells, 2003, 21(1): 50-60.
18.	Malkowski A, Sobolewski K, Jaworski S, et al. FGF binding by extracellular matrix components of Wharton's jelly. Acta Biochim Pol, 2007, 54(2): 357-63.
19.	Angelucci S, Marchisio M, Di Giuseppe F, et al. Proteome analysis of human Wharton's jelly cells during in vitro expansion. Proteome Sci, 2010, 26: 8: 18.
20.	Friedman R, Betancur M, Boissel L, et al. Umbilical cord mesenchymal stem cells: adjuvants for human cell transplantation. Biol. Biol Blood Marrow Transplant, 2007, 13(12): 1477-86.

1. Yuan D, Bao Y, El-Hashash A. Mesenchymal stromal cell-based therapy in lung diseases; from research to clinic. Am J Stem Cells, 2024, 13(2): 37-58.
2. 朱潔云, 高敏, 葉長廣, 等. 慢性阻塞性肺疾病患者一年非計劃再入院風險預測模型的構建. 中國呼吸與危重監護雜志, 2025, 24(1): 1-8.
3. 曾黎靜, 張嘉瑞, 易群. 慢性阻塞性肺疾病急性加重期合并II型呼吸衰竭列線圖模型的構建及驗證. 中國呼吸與危重監護雜志. 2024, 23 (12): 876-881.
4. Ma J, Li G, Wang H, et al. Comprehensive review of potential drugs with anti-pulmonary fibrosis properties. Biomed Pharmacother, 2024, 173: 116282.
5. Bai X, Chen Q, Li F, et al. Optimized inhaled LNP formulation for enhanced treatment of idiopathic pulmonary fibrosis via mRNA-mediated antibody therapy. Nat Commun, 2024, 15(1): 6844.
6. Wang Y, Wang L, Ma S, et al. Repair and regeneration of the alveolar epithelium in lung injury. FASEB J. 2024, 38(8): e23612.
7. Brown C, McKee C, Bakshi S, et al. Mesenchymal stem cells: Cell therapy and regeneration potential. J Tissue Eng Regen Med, 2019, 13(9): 1738-1755.
8. Shaikh MS, Shahzad Z, Tash EA, et al. Human Umbilical Cord Mesenchymal Stem Cells: Current Literature and Role in Periodontal Regeneration. Cells, 2022, 11(7): 1168.
9. 毛壯, 王華. 間充質干細胞治療肺損傷研究進展. 軍事醫學, 2023, 4(9): 714-720.
10. 趙培, 郭石平. 黑色素瘤缺乏因子2炎癥小體在非感染性肺部疾病中的研究進展. 中國呼吸與危重監護雜志, 2025, 24(2): 147-152.
11. Saito A, Horie M, Micke P, et al. The Role of TGF-β Signaling in Lung Cancer Associated with Idiopathic Pulmonary Fibrosis. Int J Mol Sci, 2018, 19(11): 3611.
12. Weis VG, Deal AC, Mekkey G, et al. Human placental-derived stem cell therapy ameliorates experimental necrotizing enterocolitis. Am J Physiol Gastrointest Liver Physiol, 2021, 320(4): G658-G674.
13. Liu N, Cheng Y, Wang D, et al. Tissue-specific populations from amniotic fluid-derived mesenchymal stem cells manifest variant in vitro and in vivo properties. Hum Cell, 2024, 37(2): 408-419.
14. Fong CY, Chak LL, Subramanian A, et al. A three dimensional anchorage independent in vitro system for the prolonged growth of embryoid bodies to study cancer cell behaviour and anticancer agents. Stem Cell Rev Rep, 2009, 5(4): 410-9.
15. Ayuzawa R, Doi C, Rachakatla RS, et al. Naive human umbilical cord matrix derived stem cells significantly attenuate growth of human breast cancer cells in vitro and in vivo. Cancer Lett, 2009, 280(1): 31-7.
16. Chen MY, Lie PC, Li ZL, et al. Endothelial differentiation of Wharton's jelly-derived mesenchymal stem cells in comparison with bone marrow-derived mesenchymal stem cells. Exp Hematol, 2009, 37(5): 629-40.
17. Mitchell KE, Weiss ML, Mitchell BM, et al. Matrix cells from Wharton's jelly form neurons and glia. Stem Cells, 2003, 21(1): 50-60.
18. Malkowski A, Sobolewski K, Jaworski S, et al. FGF binding by extracellular matrix components of Wharton's jelly. Acta Biochim Pol, 2007, 54(2): 357-63.
19. Angelucci S, Marchisio M, Di Giuseppe F, et al. Proteome analysis of human Wharton's jelly cells during in vitro expansion. Proteome Sci, 2010, 26: 8: 18.
20. Friedman R, Betancur M, Boissel L, et al. Umbilical cord mesenchymal stem cells: adjuvants for human cell transplantation. Biol. Biol Blood Marrow Transplant, 2007, 13(12): 1477-86.

Chinese Journal of Respiratory and Critical Care Medicine

Study on the biological characteristics of umbilical cord mesenchymal stem cells and their reparative effects on bleomycin-induced acute lung injury in mice

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