Analysis of iron acquisition and transportation system in clinical <i>Klebsiella pneumoniae</i> isolates _Chinese Journal of Evidence-Based Medicine

Authors：

ZHANG Jie , HUANG Xiangning , LIU Xin ,  YU Hua

Department of Clinical Laboratory Medicine, Sichuan Academy of Medical Sciences; Sichuan Provincial People's Hospital, Chengdu, 610072, P.R.China;

Corresponding?author：

YU Hua, Email: yvhua2002@163.com

Keywords：

Klebsiella pneumoniae; Iron system gene; Next generation sequencing; Pathogenicity

DOI：

10.7507/1672-2531.201801039

Video：

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ObjectiveTo evaluate the relationship between the genes involved in regulating iron uptake and maintaining iron homeostasis in Klebsiella pneumoniae from different sources and pathogenicity.MethodsThe genomic DNA sequences of two strains of Klebsiella pneumoniae from different sources were sequenced, stitched together, annotated and analyzed by second generation sequencing technique. The transversal comparison between different types of Klebsiella pneumoniae in NCBI database of iron carrier gene cluster iroB/C/D.ResultsIn these two Klebsiella pneumoniae clinical strains, the strain isolated from liver abscess patient carried 11 different iron acquisition and transportation system specific genes, which were not found in the non-liver abscess patient strain. Combined with the analysis of this sequence, in the NCBI database, six different strains of Klebsiella pneumoniae showed iroB/C/D triple positive.ConclusionIron acquisition and transportation system in Klebsiella pneumoniae may be an important pathogenic factor, which is closely related to hepatic abscess.

Citation： ZHANG Jie, HUANG Xiangning, LIU Xin, YU Hua. Analysis of iron acquisition and transportation system in clinical Klebsiella pneumoniae isolates . Chinese Journal of Evidence-Based Medicine, 2018, 18(3): 276-279. doi: 10.7507/1672-2531.201801039 Copy

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10.	吳華, 李東冬, 王京, 等. 肺炎克雷伯菌肝膿腫的臨床及微生物特征分析. 中華醫學雜志, 2015, 95(40): 3259-3263.
11.	汪強, 葛玉梅, 劉建芳, 等. 肝膿腫高毒力肺炎克雷伯菌耐藥性及毒力基因型流行性分析. 中華醫院感染學雜志, 2016, 26(24): 5531-5533, 5586.
12.	馬榮, 劉科芳, 聶大平, 等. 肺炎克雷伯菌中 K1、K2 血清型菌株的分布及其耐藥性分析. 中國感染與化療雜志, 2015, 15(3): 204-208.
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14.	陳楊, 劉嘉琳, 瞿洪平. 鐵載體對肺炎克雷伯菌毒力增強的機制研究. 中國感染與化療雜志, 2016, 16(6): 804-807.
15.	Ye M, Tu J, Jiang J, et al. Clinical and genomic analysis of liver abscess-causing klebsiella pneumoniae identifies new liver abscess-associated virulence genes. Front Cell Infect Microbiol, 2016, 6: 165.

1. Liu Y, Wang JY, Jiang W. An increasing prominent disease of Klebsiella pneumoniae liver abscess: etiology, diagnosis, and treatment. Gastroenterol Res Pract, 2013, 2013: 258514.
2. Lin YC, Lu MC, Tang HL, et al. Assessment of hypermucoviscosity as a virulence factor for experimental Klebsiella pneumoniae infections: comparative virulence analysis with hypermucoviscosity-negative strain. BMC Microbiol, 2011, 11: 50.
3. Nairz M, Haschka D, Demetz E, et al. Iron at the interface of immunity and infection. Front Pharmacol, 2014, 5: 152.
4. Cassat JE, Skaar EP. Iron in infection and immunity. Cell Host Microbe, 2013, 13(5): 509-519.
5. 張杰, 喻華. 四川省人民醫院肝膿腫患者血培養分離肺炎克雷伯桿菌鐵系統基因攜帶情況的分析. 中國循證醫學雜志, 2017, 17(8): 907-909.
6. Russo TA, Olson R, Macdonald U, et al. Aerobactin mediates virulence and accounts for increased siderophore production under iron-limiting conditions by hypervirulent (hypermucoviscous) Klebsiella pneumoniae. Infect Immun, 2014, 82(6): 2356-2367.
7. Holt KE, Wertheim H, Zadoks RN, et al. Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health. Proc Natl Acad Sci USA, 2015, 112(27): E3574-3581.
8. 尹大龍, 劉連新. 細菌性肝膿腫診治進展. 中國實用外科雜志, 2013, 33(9): 793-795.
9. 陳帆, 張艷亭, 喬慧捷, 等. 肺炎克雷伯菌肝膿腫的臨床特征分析. 臨床肝膽病雜志, 2016, 32(4): 764-768.
10. 吳華, 李東冬, 王京, 等. 肺炎克雷伯菌肝膿腫的臨床及微生物特征分析. 中華醫學雜志, 2015, 95(40): 3259-3263.
11. 汪強, 葛玉梅, 劉建芳, 等. 肝膿腫高毒力肺炎克雷伯菌耐藥性及毒力基因型流行性分析. 中華醫院感染學雜志, 2016, 26(24): 5531-5533, 5586.
12. 馬榮, 劉科芳, 聶大平, 等. 肺炎克雷伯菌中 K1、K2 血清型菌株的分布及其耐藥性分析. 中國感染與化療雜志, 2015, 15(3): 204-208.
13. 和晉渝. 肺炎克雷伯菌的血清分型及毒力基因分布的研究. 重慶: 重慶醫科大學, 2012.
14. 陳楊, 劉嘉琳, 瞿洪平. 鐵載體對肺炎克雷伯菌毒力增強的機制研究. 中國感染與化療雜志, 2016, 16(6): 804-807.
15. Ye M, Tu J, Jiang J, et al. Clinical and genomic analysis of liver abscess-causing klebsiella pneumoniae identifies new liver abscess-associated virulence genes. Front Cell Infect Microbiol, 2016, 6: 165.

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