脂肪組織來源干細胞的分化潛能和應用_《中國修復重建外科雜志》

作者：

史琳麗 ¹ , 楊向群 ²

第二軍醫大學（上海，200433）1臨床2隊，2解剖學教研室生物醫學工程研究所;

關鍵詞：

脂肪來源干細胞去分化脂肪細胞血管基質成分再生醫學

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目的介紹脂肪組織來源的干細胞種類、分化潛能及在再生醫學中的應用和優勢。方法廣泛查閱近年關于BMSCs、脂肪來源干細胞（adipose-derived stem cells，ADSCs）和去分化脂肪（dedifferentiated fat，DFAT）細胞的實驗研究及臨床研究文獻，并進行整理、綜合與分析。結果從脂肪基質成分可以分離得到ADSCs，ADSCs具有多向分化潛能，可分化為脂肪、骨、軟骨、內皮、肌以及神經細胞等，并已較成功地應用于再生醫學各領域。成熟脂肪細胞經過天花板培養法可以去分化為成纖維樣細胞，即DFAT細胞，獲得了多向分化潛能，也可以像ADSCs一樣分化為脂肪、骨、軟骨、內皮、肌以及神經細胞等。相比較目前常用的成體干細胞BMSCs，ADSCs、DFAT細胞來源廣泛、取材更容易；而相對于ADSCs，DFAT細胞均一性高，增殖能力強。結論脂肪組織作為人體干細胞的重要來源，可能會給臨床組織缺損的修復與再生帶來新希望。

引用本文： 史琳麗,楊向群. 脂肪組織來源干細胞的分化潛能和應用. 中國修復重建外科雜志, 2012, 26(8): 1007-1011. doi: 復制

1.	Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell based therapied. Tissue Eng, 2001, 7(2): 211-228．.
2.	Schächinger V, Erbs S, Elsässer A, et al. Improved clinical outcome after intracoronary administration of bone marrow-derived progenitor cells in acute myocardial infarction: final 1-year results of the REPAIR-AMI trial. Eur Heart J, 2006, 27(23): 2775-2783．.
3.	Chen J, Larsson L, Haugen E, et al. Effects of autoantibodies removed by immunoadsorption from patients with dilated cardiomyopathy on neonatal rat cardiomyocytes. Eur J Heart Fail, 2006, 8(5): 460-467.
4.	Suga H, Matsumoto D, Inoue K, et al. Numerical measurement of viable and nonviable adipocytes and other cellular components in aspirated fat tissue. Plast Reconstr Surg, 2008, 122(1): 103-114.
5.	Garcia-Olmo D, Herreros D, Pascual M, et al. Treatment of enterocutaneous fistula in Crohn’s Disease with adipose-derived stem cells: a comparison of protocols with and without cell expansion. Int J Colorectal Dis, 2009, 24(1): 27-30.
6.	Riordan NH, Ichim TE, Min WP, et al. Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis. J Transl Med, 2009, 7: 29.
7.	梁星, 朱輝. 脂肪來源干細胞的應用研究進展. 中國美容醫學, 2010, 19(11): 1734-1737.
8.	Wickham MQ, Erickson GR, Gimble JM, et al. Multipotent stromal cells derived from the infrapatellar fat pad of the knee. Clin Orthop Relat Res, 2003, (412): 196-212.
9.	Al-Salleeh F, Beatty MW, Reinhardt RA, et al. Human osteogenic protein-1 induces osteogenic differentiation of adipose-derived stem cells harvested from mice. Arch Oral Biol, 2008, 53(10): 928-936.
10.	Lendeckel S, Jödicke A, Christophis P, et al. Autologous steam cells (adipose) and fibrin glue used to treat wide spread traumatic calvarial defect: case report. J Craniomaxillofac Surg, 2004, 32(6): 370-373.
11.	Jin X, Sun Y, Zhang K, et al. Ectopic neocartilage formation from predifferentiated human adipose derived stem cells induced by adenoviral-mediated transfer of hTGF beta2. Biomaterials, 2007, 28(19): 2994-3003.
12.	Afizah H, Yang Z, Hui JH, et al. A comparison between the chendrngenic potential of human bone marrow stem cells(BMSCs)and adipose-derived stem cells(ADSCs) taken from same donor. Tissue Eng, 2007, 13(4): 659-666.
13.	Cao Y, Sun Z, Liao L, et al. Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo. Biochem Biophys Res Commun, 2005, 332(2): 370-379.
14.	Planat-Bénard V, Silvestre JS, Cousin B, et al. Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives. Circulation, 2004, 109(5): 656-663.
15.	Schenke-Layland K, Strem BM, Jordan MC, et al. Adipose tissue-derived cells improve cardiac function following myocardial infarction. J Surg Res, 2009, 153(2): 217-223.
16.	Planat-Bénard V, Menard C, André M, et al. Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells. Circ Res, 2004, 94(2): 223-229.
17.	Miyahara Y, Nagaya N, Kataoka M, et al. Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction. Nat Med, 2006, 12(4): 459-465.
18.	Vieira NM, Brandalise V, Zucconi E, et al. Human multipotent adipose-derived stem cells restore dystrophin expression of Duchenne skeletal-muscle cells in vivo. Biol Cell, 2008, 100(4): 231-241.
19.	王剛, 曹衛剛. 具有多向分化潛能的脂肪干細胞研究的新進展. 組織工程與重建外科雜志, 2009, 5(3): 173-176.
20.	Sugihara H, Yonemitsu N, Miyabara S, et al. Primary cultures of unilocular fat cells: characteristics of growth in vitro and changes in differentiation properties. Differentiation, 1986, 31(1): 42-49.
21.	Matsumoto T, Kano K, Kondo D, et al. Mature adipocyte- derived dedifferentiated fat cells exhibit multilineage potential. J Cell Physiol, 2008, 215(1): 210-222.
22.	Shen J, Sugawara A, Yamashita J, et al. Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues. Int J Oral Sci, 2011, 3(3): 117-124.
23.	Yagi K, Kondo D, Okazaki Y, et al. A novel preadipocyte cell line established from mouse adult mature adipocytes. Biochem Biophys Res Commun, 2004, 321(4): 967-974.
24.	Nobusue H, Endo T, Kano K, et al. Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue. Cell Tissue Res, 2008, 332(3): 435-446.
25.	Jumabay M, Zhang R, Yao Y, et al. Spontaneously beating cardiomyocytes derived from white mature adipocytes. Cardiovas Res, 2010, 85(1): 17-27.
26.	Obinata D, Matsumoto T, Ikado Y, et al. Transplantation of mature adipocyte-derived dedifferentiated fat (DFAT) cells improves urethral sphincter contractility in a rat model. Int J Urol, 2011, 18(12): 827-834.
27.	Jumabay M, Matsumoto T, Yokoyama S, et al. Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats. J Mol Cell Cardiol, 2009, 47(5): 565-575.
28.	Ohta Y, Takenaga M, Tokura Y, et al. Mature adipocyte-derived cells, dedifferentiated fat cells (DFAT), promoted functional recovery from spinal cord injury-induced motor dysfunction in rats. Cell Transplant, 2008, 17(8): 877-886.
29.	李春明, 劉毅. 體外的培養的人脂肪間充質干細胞生物學特性的研究. 中國美容醫學, 2007, 16(2): 159-162.
30.	朱希山, 施薇, 臺衛平, 等. 脂肪與骨髓來源間充質干細胞生物學特性的比較. 中國組織工程研究與臨床康復, 2011, 15(32): 5937-5940.
31.	周進, 田國萍, 王靜娥, 等. 脂肪基質干細胞和骨髓基質干細胞的體外分化能力. 中國組織工程研究與臨床康復, 2011, 15(32): 5932-5935.
32.	Al-Nbaheen M, Vishnubalaji R, Ali D, et al. Human stromal(mesenchymal) stem cells from bone marrow, adipose tissue and skin exhibit differences in molecular phenotype and differentiation potential. Stem Cell Rev, 2012. [Epub ahead of print].
33.	Lee JM, Jung J, Lee HJ, et al. Comparison of immunomodulatory effects of placenta stem cells with bone marrow and adipose mesenchymal stem cells. Int Immunopharmacol, 2012, 13(2): 219-224.
34.	朱希山, 臺衛平, 施薇, 等. 骨髓和脂肪來源間充質干細胞的免疫調節作用. 中國組織工程研究與臨床康復, 2011, 15(36): 6683-6686.
35.	Zhu Y, Liu T, Song K, et al. Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem Funct, 2008, 26(6): 664-675.
36.	Gao Q, Zhao L, Song Z, et al. Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs. Mol Biol Rep, 2012, 39(5): 5791-5804.

1. Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell based therapied. Tissue Eng, 2001, 7(2): 211-228．.
2. Schächinger V, Erbs S, Elsässer A, et al. Improved clinical outcome after intracoronary administration of bone marrow-derived progenitor cells in acute myocardial infarction: final 1-year results of the REPAIR-AMI trial. Eur Heart J, 2006, 27(23): 2775-2783．.
3. Chen J, Larsson L, Haugen E, et al. Effects of autoantibodies removed by immunoadsorption from patients with dilated cardiomyopathy on neonatal rat cardiomyocytes. Eur J Heart Fail, 2006, 8(5): 460-467.
4. Suga H, Matsumoto D, Inoue K, et al. Numerical measurement of viable and nonviable adipocytes and other cellular components in aspirated fat tissue. Plast Reconstr Surg, 2008, 122(1): 103-114.
5. Garcia-Olmo D, Herreros D, Pascual M, et al. Treatment of enterocutaneous fistula in Crohn’s Disease with adipose-derived stem cells: a comparison of protocols with and without cell expansion. Int J Colorectal Dis, 2009, 24(1): 27-30.
6. Riordan NH, Ichim TE, Min WP, et al. Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis. J Transl Med, 2009, 7: 29.
7. 梁星, 朱輝. 脂肪來源干細胞的應用研究進展. 中國美容醫學, 2010, 19(11): 1734-1737.
8. Wickham MQ, Erickson GR, Gimble JM, et al. Multipotent stromal cells derived from the infrapatellar fat pad of the knee. Clin Orthop Relat Res, 2003, (412): 196-212.
9. Al-Salleeh F, Beatty MW, Reinhardt RA, et al. Human osteogenic protein-1 induces osteogenic differentiation of adipose-derived stem cells harvested from mice. Arch Oral Biol, 2008, 53(10): 928-936.
10. Lendeckel S, Jödicke A, Christophis P, et al. Autologous steam cells (adipose) and fibrin glue used to treat wide spread traumatic calvarial defect: case report. J Craniomaxillofac Surg, 2004, 32(6): 370-373.
11. Jin X, Sun Y, Zhang K, et al. Ectopic neocartilage formation from predifferentiated human adipose derived stem cells induced by adenoviral-mediated transfer of hTGF beta2. Biomaterials, 2007, 28(19): 2994-3003.
12. Afizah H, Yang Z, Hui JH, et al. A comparison between the chendrngenic potential of human bone marrow stem cells(BMSCs)and adipose-derived stem cells(ADSCs) taken from same donor. Tissue Eng, 2007, 13(4): 659-666.
13. Cao Y, Sun Z, Liao L, et al. Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo. Biochem Biophys Res Commun, 2005, 332(2): 370-379.
14. Planat-Bénard V, Silvestre JS, Cousin B, et al. Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives. Circulation, 2004, 109(5): 656-663.
15. Schenke-Layland K, Strem BM, Jordan MC, et al. Adipose tissue-derived cells improve cardiac function following myocardial infarction. J Surg Res, 2009, 153(2): 217-223.
16. Planat-Bénard V, Menard C, André M, et al. Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells. Circ Res, 2004, 94(2): 223-229.
17. Miyahara Y, Nagaya N, Kataoka M, et al. Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction. Nat Med, 2006, 12(4): 459-465.
18. Vieira NM, Brandalise V, Zucconi E, et al. Human multipotent adipose-derived stem cells restore dystrophin expression of Duchenne skeletal-muscle cells in vivo. Biol Cell, 2008, 100(4): 231-241.
19. 王剛, 曹衛剛. 具有多向分化潛能的脂肪干細胞研究的新進展. 組織工程與重建外科雜志, 2009, 5(3): 173-176.
20. Sugihara H, Yonemitsu N, Miyabara S, et al. Primary cultures of unilocular fat cells: characteristics of growth in vitro and changes in differentiation properties. Differentiation, 1986, 31(1): 42-49.
21. Matsumoto T, Kano K, Kondo D, et al. Mature adipocyte- derived dedifferentiated fat cells exhibit multilineage potential. J Cell Physiol, 2008, 215(1): 210-222.
22. Shen J, Sugawara A, Yamashita J, et al. Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues. Int J Oral Sci, 2011, 3(3): 117-124.
23. Yagi K, Kondo D, Okazaki Y, et al. A novel preadipocyte cell line established from mouse adult mature adipocytes. Biochem Biophys Res Commun, 2004, 321(4): 967-974.
24. Nobusue H, Endo T, Kano K, et al. Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue. Cell Tissue Res, 2008, 332(3): 435-446.
25. Jumabay M, Zhang R, Yao Y, et al. Spontaneously beating cardiomyocytes derived from white mature adipocytes. Cardiovas Res, 2010, 85(1): 17-27.
26. Obinata D, Matsumoto T, Ikado Y, et al. Transplantation of mature adipocyte-derived dedifferentiated fat (DFAT) cells improves urethral sphincter contractility in a rat model. Int J Urol, 2011, 18(12): 827-834.
27. Jumabay M, Matsumoto T, Yokoyama S, et al. Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats. J Mol Cell Cardiol, 2009, 47(5): 565-575.
28. Ohta Y, Takenaga M, Tokura Y, et al. Mature adipocyte-derived cells, dedifferentiated fat cells (DFAT), promoted functional recovery from spinal cord injury-induced motor dysfunction in rats. Cell Transplant, 2008, 17(8): 877-886.
29. 李春明, 劉毅. 體外的培養的人脂肪間充質干細胞生物學特性的研究. 中國美容醫學, 2007, 16(2): 159-162.
30. 朱希山, 施薇, 臺衛平, 等. 脂肪與骨髓來源間充質干細胞生物學特性的比較. 中國組織工程研究與臨床康復, 2011, 15(32): 5937-5940.
31. 周進, 田國萍, 王靜娥, 等. 脂肪基質干細胞和骨髓基質干細胞的體外分化能力. 中國組織工程研究與臨床康復, 2011, 15(32): 5932-5935.
32. Al-Nbaheen M, Vishnubalaji R, Ali D, et al. Human stromal(mesenchymal) stem cells from bone marrow, adipose tissue and skin exhibit differences in molecular phenotype and differentiation potential. Stem Cell Rev, 2012. [Epub ahead of print].
33. Lee JM, Jung J, Lee HJ, et al. Comparison of immunomodulatory effects of placenta stem cells with bone marrow and adipose mesenchymal stem cells. Int Immunopharmacol, 2012, 13(2): 219-224.
34. 朱希山, 臺衛平, 施薇, 等. 骨髓和脂肪來源間充質干細胞的免疫調節作用. 中國組織工程研究與臨床康復, 2011, 15(36): 6683-6686.
35. Zhu Y, Liu T, Song K, et al. Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem Funct, 2008, 26(6): 664-675.
36. Gao Q, Zhao L, Song Z, et al. Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs. Mol Biol Rep, 2012, 39(5): 5791-5804.

《中國修復重建外科雜志》

脂肪組織來源干細胞的分化潛能和應用

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《中國修復重建外科雜志》

脂肪組織來源干細胞的分化潛能和應用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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